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HaloTag-dSpCas9

Plasmid

#238946

Purpose

N-terminal fusion of HaloTag to dSpCas9, mammalian expression

Depositor

David Segal

Article

Heath et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

HaloTag

Tags

dSpCas9

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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dSaCas9-NLuc

Plasmid

#238947

Purpose

C-terminal fusion of NanoLuc luciferase to dSaCas9, mammalian expression

Depositor

David Segal

Article

Heath et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

NanoLuc

Tags

dSaCas9

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBIG2ABC-eIF3Δbgi

Plasmid

#240043

Purpose

Human eIF3k, eIF3l, eIF3e, eIF3f, eIF3h, eIF3d, eIF3m, eIF3a and eIF3c expression in insect cells for purification

Depositor

Venki Ramakrishnan

Article

Diaz-Lopez et al Protein Sci. 2026 Jan;35(1):e70388. doi:

Insert

eIF3k, l, e, f, h, d, m, a, c

Tags

eIF3a: TEV_3xFLAG, eIF3c: 3C_STREP II, eIF3d: HA

Expression

Insect

Mutation

WT

Promoter

Polyhedrin

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pACEBac1-eIF3c-3C-STREP

Plasmid

#240026

Purpose

Human eIF3c for further cloning or expression in insect cells for purification

Depositor

Venki Ramakrishnan

Article

Diaz-Lopez et al Protein Sci. 2026 Jan;35(1):e70388. doi:

Insert

eIF3c (EIF3C Human)

Tags

3C_STREP II

Expression

Insect

Mutation

WT

Promoter

Polyhedrin

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pMD18T-AtU6-HV5

Plasmid

#239470

Purpose

Encodes a gRNA expression cassette driven by the AtU6 promoter.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

U6-gRNA

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMD18T-AtU3b-HV5

Plasmid

#239477

Purpose

Encodes a gRNA expression cassette driven by the AtU3b promoter.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

U3b-gRNA

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMD18T-At7SL-HV5

Plasmid

#239478

Purpose

Encodes a gRNA expression cassette driven by the At7SL promoter.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

7SL-gRNA

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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p1300-dCas9-HV5

Plasmid

#239479

Purpose

An intermediate vector is used to assemble three gRNAs via Golden Gate cloning.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

UBQ-dCAS9

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pANC98a(3xFLAG-NES-FKBP12-(G4S)4-nTEV-IRES-dTomato)

Plasmid

#248358

Purpose

Lentiviral vector that can express FKBP12 along with N terminal split TEV. Expressed off of CMV tet ON promoter with dTomato marker

Depositor

Arnab Mukherjee

Article

Chacko et al bioRxiv 2025.09.07.674790

Insert

3xFLAG-NES-FKBP12-(G4S)4x-nTEV

Use

Lentiviral

Promoter

tight TRE

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGGE-AtHAC1cd

Plasmid

#246809

Purpose

A GreenGate entry vector containing coding region of AtHAC1 catalytic domain

Depositor

Danhua Jiang

Insert

AtHAC1cd (HAC1 Mustard Weed)

Use

Greengate cloning entry vector

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pYSGDLuc

Plasmid

#236297

Purpose

Dual luciferase reporter for measuring recoding efficiencies in yeast

Depositor

Pasha Baranov

Article

Fenton et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Type

Empty backbone

Expression

Yeast

Promoter

TEF1

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pRSET-IRES-EGFP

Plasmid

#216151

Purpose

Donor of EMCV IRES for the preparation of other plasmids.

Depositor

Tom Brown

Article

Golojuch et al Nat Commun. 2025 Oct 16;16(1):9205. doi:

Inserts

EMCV IRES
EGFP

Use

Synthetic Biology; Mrna preparation

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pColE1-MP-Violacein

Plasmid

#240205

Purpose

Violacein biosynthesis pathway induced by CRISPRa

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

vioA-vioB-vioC-vioD-vioE

Expression

Bacterial

Promoter

J3-DA9-UP1-MP1

Available Since

Nov. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pSC-MRscRNA

Plasmid

#240211

Purpose

Yeast vector expressing theophylline-responsive MR-scRNA

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

Theophylline MR-scRNA targeting TetO

Expression

Yeast

Promoter

SNR52

Available Since

Nov. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pHS-dCas9

Plasmid

#240212

Purpose

Mammalian vector for transient expression of dCas9

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

dCas9

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pHS-MCP-VPR

Plasmid

#240213

Purpose

Mammalian vector for transient expression of MCP-VPR

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

MCP-VPR

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pHS-MRscRNA

Plasmid

#240214

Purpose

Mammalian vector for transient expression of theophylline-responsive MR-scRNA with mCherry transfection marker

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

Theophylline MR-scRNA targeting TET3G promoter

Expression

Mammalian

Promoter

U6

Available Since

Nov. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pColE1-MP-mRFP1

Plasmid

#240203

Purpose

Medium copy mRFP1 reporter for monitoring metabolite-responsive CRISPRa activity

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

mRFP1

Expression

Bacterial

Promoter

J3-DA9-UP1-MP1

Available Since

Nov. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pMITO-mTFP1

Plasmid

#244030

Purpose

anchoring mTFP1 to the mitochondrial membrane

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

CISD1-mTFP1

Tags

sunTagV4

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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mito_mCherry_HA

Plasmid

#244042

Purpose

anchoring mCherry-1x gp41MoonTag to the mitochondrial membrane

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

CISD1-mCherry-HA

Tags

HA

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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