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UBC-TLR4-HA hygro

Plasmid

#246905

Purpose

Expresses HA-tagged human TLR4 in mammalian cells

Depositor

William Kaelin

Article

Lampson et al Cell. 2024 Apr 25;187(9):2209-2223.e16.

Insert

TLR4 (TLR4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

UBC

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pTW411c

Plasmid

#246584

Purpose

different combination of A. thaliana and N. tabacum Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

NtRaf1, NtRaf2, NtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (CPN60B, CPN60A, CPN20 Mustard Weed)

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pYO391

Plasmid

#235751

Purpose

Protein expression of ScVPS38, untagged + ScVPS34, untagged + FL ScVPS15-ZZ + ZZ-FL ScVPS30

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS34 (VPS34 Budding Yeast)
VPS15 (VPS15 Budding Yeast)
VPS38 (VPS38 Budding Yeast)
VPS30 (VPS30 Budding Yeast)

Tags

3xTEV-ZZ and ZZ-3xTEV

Expression

Yeast

Mutation

S2A and T134A, I851R

Promoter

GALGAPDH

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-FAM136A-WT-3xFLAG-hygro

Plasmid

#244880

Purpose

Overexpression of wild-type human FAM136A

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A (FAM136A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-HP1γ-W174A-EGFP

Plasmid

#179933

Purpose

C terminal fusion of EGFP to mouse HP1γ (Cbx3) containing W174A mutation

Depositor

Andrew Newman

Article

Newman et al Nat Commun. 2025 Aug 8;16(1):7325. doi:

Insert

Cbx 3 (Cbx3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

W174A

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-HP1α-F44E-EGFP

Plasmid

#179936

Purpose

C terminal fusion of EGFP to mouse HP1α (Cbx5) containing F44E mutation

Depositor

Andrew Newman

Article

Newman et al Nat Commun. 2025 Aug 8;16(1):7325. doi:

Insert

cbx5 (Cbx5 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

F44E

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-HP1α-I165E-EGFP

Plasmid

#179937

Purpose

C terminal fusion of GFP to mouse HP1α (Cbx5) containing I165E mutation

Depositor

Andrew Newman

Article

Newman et al Nat Commun. 2025 Aug 8;16(1):7325. doi:

Insert

cbx5 (Cbx5 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

I165E

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-HP1β-eGFP

Plasmid

#179925

Purpose

C terminal fusion of EGFP to mouse HP1β (Cbx1)

Depositor

Andrew Newman

Article

Newman et al Nat Commun. 2025 Aug 8;16(1):7325. doi:

Insert

Cbx1 (Cbx1 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-HP1γ-M158V-EGFP

Plasmid

#179927

Purpose

C terminal fusion of EGFP to mouse HP1γ (Cbx3) containing M158V mutation

Depositor

Andrew Newman

Article

Newman et al Nat Commun. 2025 Aug 8;16(1):7325. doi:

Insert

cbx 3 (Cbx3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

M158V

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-HP1γ-I165E-EGFP

Plasmid

#179929

Purpose

C terminal fusion of EGFP to mouse HP1γ (Cbx3) containing I165E mutation

Depositor

Andrew Newman

Article

Newman et al Nat Commun. 2025 Aug 8;16(1):7325. doi:

Insert

Cbx 3 (Cbx3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

I165E

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-HP1γ-F167R-EGFP

Plasmid

#179930

Purpose

C terminal fusion of EGFP to mouse HP1γ (Cbx3) containing F167R mutation

Depositor

Andrew Newman

Article

Newman et al Nat Commun. 2025 Aug 8;16(1):7325. doi:

Insert

Cbx 3 (Cbx3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

F167R

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-HP1γ-E170V-EGFP

Plasmid

#179931

Purpose

C terminal fusion of EGFP to mouse HP1γ (Cbx3) containing E170V mutation

Depositor

Andrew Newman

Article

Newman et al Nat Commun. 2025 Aug 8;16(1):7325. doi:

Insert

Cbx 3 (Cbx3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

E170V

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-HP1γ-L172H-EGFP

Plasmid

#179932

Purpose

C terminal fusion of EGFP to mouse HP1γ (Cbx3) containing L172H mutation

Depositor

Andrew Newman

Article

Newman et al Nat Commun. 2025 Aug 8;16(1):7325. doi:

Insert

Cbx 3 (Cbx3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

L172H

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Ub-Myc-LgBiT

Plasmid

#241817

Purpose

Expresses Myc-tagged and LgBiT-tagged Ubiquitin for use with the Promega HiBiT system

Depositor

Ethan Lee

Article

Kassel et al Nat Commun. 2025 Jun 5;16(1):5248. doi:

Insert

Ubiquitin

Tags

LgBiT and Myc

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pRP-B-catenin-V5-LgBiT

Plasmid

#241816

Purpose

Expresses V5-tagged and LgBiT-tagged B-catenin for use with the Promega HiBiT system

Depositor

Ethan Lee

Article

Kassel et al Nat Commun. 2025 Jun 5;16(1):5248. doi:

Insert

B-catenin

Tags

LgBiT and V5

Expression

Mammalian

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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p-HITI-F9-Ckm

Plasmid

#226120

Purpose

HITI insert construct with Ckm gRNA and hF9 transgene

Depositor

Christopher Nelson

Article

Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

Inserts

F9 (F9 Human)
Ckm gRNA (Ckm Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-ITR-CMV-mCherry-1xU6-LeuIGI1

Plasmid

#217367

Purpose

Expresses E. coli leucine tRNA variant "LeuIGI1" and a wild-type mCherry reporter; can be packaged into AAV

Depositor

Abhishek Chatterjee

Article

Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2

Insert

E. coli leucine tRNA mutant, "LeuIGI1" for TAG suppression

Use

AAV

Expression

Mammalian

Mutation

G6U, C78G, U83C

Promoter

U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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CD44v-EC-His (pcDNA3)

Plasmid

#238418

Purpose

Expresses the polyhistidine-tagged extracellular region of CD44v (CD44v-EC-His)

Depositor

Amy Shiu Lee

Article

Tseng et al Life Sci Alliance. 2019 Aug 15;2(4):e201

Insert

CD44 containing extracellular region of CD44v (CD44 Human)

Tags

His

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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lentiGuide-chr20-49345720-gRNA1

Plasmid

#232623

Purpose

Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.

Depositor

Claes Wadelius

Article

Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1

Insert

S. pyogenes sgRNA cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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lentiGuide-chr20-49345720-gRNA2

Plasmid

#232624

Purpose

Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.

Depositor

Claes Wadelius

Article

Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1

Insert

S. pyogenes sgRNA cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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