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pGL0_5 [pAL5000 ORI]

Plasmid

#198922

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pAL5000 ORI

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pSFFV_mNG3K(1-10)

Plasmid

#157993

Purpose

Lentiviral vector of constitutive expression of mNeonGreen3K(1-10)

Depositor

Bo Huang

Article

Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

Insert

pSFFV_mNG3K(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

G27D, D42E, M128I, K153M, T170I

Promoter

SFFV

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTubulin-miRFP670-2

Plasmid

#197238

Purpose

Expresses the fusion protein of Tubulin-miRFP670-2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Tubulin-miRFP670-2

Expression

Mammalian

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRK-flag-GFP11

Plasmid

#78590

Purpose

Expression flag-GFP 11 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-GFP 11

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pTRKH3-slpGFP

Plasmid

#27168

Depositor

Michela Lizier

Article

Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

Insert

surface (S)-layer protein promoter region fused with modified green fluorescent protein 5

Expression

Bacterial

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pET24b-APEX2

Plasmid

#111702

Purpose

For IPTG-inducible expression of APEX2 in E. coli

Depositor

Jessica Seeliger

Article

Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

Insert

APEX2

Tags

6xHis and V5

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Ub-R-GFP

Plasmid

#11939

Depositor

Nico Dantuma

Article

Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

Insert

Ub-R-GFP

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of GFP. Arginine at…

Available Since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-MTM1

Plasmid

#51614

Purpose

Recruitable PtdIns3P/PtdIns(3,5)P2 phosphatase

Depositor

Tamas Balla

Article

Hammond et al J Cell Biol. 2014 Apr 7.

Inserts

FKBP
MTM1 (MTM1 Human, 1812)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pSFFV_mNG3A(1-10)

Plasmid

#157992

Purpose

Lentiviral vector of constitutive expression of mNeonGreen3A(1-10)

Depositor

Bo Huang

Article

Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

Insert

pSFFV_mNG3K(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

W148L, M150V, K153M, N194K, F204L

Promoter

SFFV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pmEGFP_a_tubulin_C1

Plasmid

#21039

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Tags

pmEGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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CIp10 - γmGFP (γmGFP based)

Plasmid

#163119

Purpose

Expresses in Candida albicans

Depositor

Patrick Van Dijck

Article

Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

Insert

gamma mGFP

Use

Candida albicans expression

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b-6His-AcpS

Plasmid

#145376

Purpose

Expression of E. coli AcpS

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

AcpS (acpP E. coli)

Tags

6-His

Expression

Bacterial

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-3xGFP-tDeg

Plasmid

#191371

Purpose

Overexpression of 3xGFP-tDeg in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

3xGFP-tDeg

Tags

3xGFP

Expression

Mammalian

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pEF cyto-cmCh

Plasmid

#188903

Purpose

Expresses cytosolic mCherry

Depositor

Edwin Chapman

Article

Vevea et al J Neurosci. 2023 May 10;43(19):3421-3438

Insert

cytosolic mCherry

Expression

Mammalian

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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GST-HA-GFP11-N1

Plasmid

#91953

Purpose

Expresses GST-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

GST-HA-GFP11

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3/HA-SUMO3 (Sentrin 2)

Plasmid

#17361

Purpose

Mammalian expression of human SUMO3 with HA tag

Depositor

Edward Yeh

Article

Kamitani et al J Biol Chem. 1998 Feb 6. 273(6):3117-20.

Insert

SUMO3 (SUMO3 Human)

Tags

HA

Expression

Mammalian

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pH2B-miRFP713-N1

Plasmid

#197230

Purpose

Expresses the fusion protein of H2B-miRFP713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

H2B-miRFP713

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-rat Nrxn1alpha

Plasmid

#58266

Purpose

Expression plasmid for HA-tagged rat Neurexin 1alpha

Depositor

Peter Scheiffele

Article

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn1alpha (Nrxn1 Rat)

Tags

HA

Expression

Mammalian

Mutation

see comments

Promoter

CAG

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR-LUC (w158-1)

Plasmid

#17473

Purpose

Gateway entry vector, Firefly Luciferase

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Entry vector

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pEB1-mCerulean

Plasmid

#103968

Purpose

Plasmid encoding mCerulean

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mCerulean

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

ProC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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