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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 264 results for: CRISPRA

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Showing: 101 - 120 of 264 results

  1. pLY134

    Plasmid
    #130962
    Purpose
    Engineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assembly
    Depositor
    Baojun Wang
    Publication
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Insert
    sgRNA-LEB5
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Anderson promoter J23100
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    130962-plasmid-map-sequence-id-257714

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  6. pLY70

    Plasmid
    #130948
    Purpose
    A CRISPR activation device with the necessary genes (dxcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter PrhaB), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Publication
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dxcas9 (3.7)
    tetR
    rhaS
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9 for dCas9; Sy…
    Promoter
    Pcon, PpspA-LEA3B3, PrhaB, and Ptet
    Available since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    130948-plasmid-map-sequence-id-257361

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  7. dSaCas9-VPR

    Plasmid
    #205251
    Purpose
    In vitro transcription plasmid for production of dSaCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #68495
    Depositor
    Rasmus Bak
    Publication
    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d
    Insert
    dSaCas9-VPR
    Use
    CRISPR
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    205251-plasmid-map-sequence-id-408439

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  13. pLY61

    Plasmid
    #130928
    Purpose
    A CRISPR activation device with the necessary genes (dxcas9 3.7 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Publication
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dxcas9 (3.7)
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9 for dCas9; Sy…
    Promoter
    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet
    Available since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    130928-plasmid-map-sequence-id-257360

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Showing: 101 - 120 of 264 results