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Plasmid#231635PurposeMammalian expression of a flag-tagged full-length version of the mouse chaperone protein proSAAS.DepositorInsertPcsk1N (Pcsk1n Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#3
Plasmid#172985PurposeCRISPRi for TAOK2DepositorInsertsgTAOK2i (TAOK2 Human)
UseCRISPR and LentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21-puro_TAOK2-D151A
Plasmid#172980Purposedoxycycline-inducible expression of kinase-dead TAOK2DepositorInsertkinase-dead TAOK2 (TAOK2-D151A) (TAOK2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationD151APromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#1
Plasmid#172983PurposeCRISPRi for TAOK2DepositorInsertsgTAOK2i (TAOK2 Human)
UseCRISPR and LentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C1 SADBs shRNA Resistant
Plasmid#67155PurposepEGFP containing N-terminally EGFP Tagged mouse SADBs resistant to an shRNADepositorInsertBrsk1 (Brsk1 Mouse)
UseTagsEGFPExpressionMammalianMutationnucleotide 294 C-T, nucleotide 297 C-GPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTER SADB shRNA Human
Plasmid#67151PurposeDoxycycline-regulated mammalian expression vector for expressing shRNA against human SADBDepositorInsertBRSK1 (BRSK1 Human)
UseRNAiTagsExpressionMammalianMutationPromoterH1 TetAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-Gnaq
Plasmid#170298PurposeEncoding mouse GnaqDepositorInsertA recombinant mouse Gnaq (Gnaq Mouse)
UseTagsN/AExpressionMammalianMutationAsilent mutation was introduced into the sgRNA-ta…PromoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
TAOK2 gRNA (BRDN0001149190)
Plasmid#77251Purpose3rd generation lentiviral gRNA plasmid targeting human TAOK2DepositorInsertTAOK2 (TAOK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TAOK2 gRNA (BRDN0001162271)
Plasmid#77252Purpose3rd generation lentiviral gRNA plasmid targeting human TAOK2DepositorInsertTAOK2 (TAOK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TAOK2 gRNA (BRDN0001148565)
Plasmid#77249Purpose3rd generation lentiviral gRNA plasmid targeting human TAOK2DepositorInsertTAOK2 (TAOK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TAOK2 gRNA (BRDN0001162486)
Plasmid#77250Purpose3rd generation lentiviral gRNA plasmid targeting human TAOK2DepositorInsertTAOK2 (TAOK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-q-YFP
Plasmid#55782PurposeThis G protein alpha-q construct contains internal insertions of YFP and the EE epitope.DepositorInsertalpha-q EE YFP (Gnaq Mouse)
UseTagsThe EE epitope was introduced into the alpha-q se…ExpressionMammalianMutationBamHI and SacI sites in alpha-q were removed with…PromoterCMVAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PAPSS1
Plasmid#20557DepositorInsertPAPSS1 (PAPSS1 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-q-GFP
Plasmid#66080PurposeThis G protein alpha-q construct contains internal insertions of GFP and the EE epitopeDepositorInsertG protein alpha-q-EE-YFP (Gnaq Mouse)
UseTagsGFP was inserted internally into the alpha-q sequ…ExpressionMammalianMutationBamHI and SacI sites in alpha-q were removed with…PromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-q-CFP
Plasmid#66081PurposeThis G protein alpha-q construct contains internal insertions of CFP and the EE epitopeDepositorInsertG protein alpha-q-EE-CFP (Gnaq Mouse)
UseTagsCFP was inserted internally into the alpha-q sequ…ExpressionMammalianMutationBamHI and SacI sites in alpha-q were removed with…PromoterCMVAvailable sinceJuly 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-PAPSS1
Plasmid#23868DepositorInsertPAPSS1 (PAPSS1 Human)
UseGateway donor vectorTagsExpressionMutation2MMPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
R777-E289 Hs.RPS6KA1
Plasmid#70573PurposeGateway ORF clone of human RPS6KA1 [NM_002953.3] with stop codon (for native or N-terminal fusions)DepositorInsertRPS6KA1 (RPS6KA1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E290 Hs.RPS6KA1-nostop
Plasmid#70574PurposeGateway ORF clone of human RPS6KA1 [NM_002953.3] without stop codon (for C-terminal fusions)DepositorInsertRPS6KA1 (RPS6KA1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
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SRPK1 (SRPK1A-c100)
Plasmid#98246PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSRPK1A (SRPK1 Human)
UseTagsHis-6-TEVExpressionBacterialMutationPromoterT7Available sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only