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pKLV2-U6gRNA5(NDUFA1(12))-PGKpuro2ABFP-W

Plasmid

#200495

Purpose

Lentiviral vector expressing gRNA targeting human NDUFA1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

NDUFA1(12) (NDUFA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(NDUFA1(11))-PGKpuro2ABFP-W

Plasmid

#200494

Purpose

Lentiviral vector expressing gRNA targeting human NDUFA1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

NDUFA1(11) (NDUFA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGST2-GST-TEV-OPTN S177D S473D

Plasmid

#187827

Purpose

Plasmid for the expression and purification of GST-TEV-OPTN S177D S473D. Internal Ref: SMC1588

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

OPTN (OPTN Human)

Use

Tags

GST-TEV

Expression

Bacterial

Mutation

S177D S473D

Promoter

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 _2-33/myc-His

Plasmid

#127492

Purpose

Expresses myc-tagged _MTS NDUFS2 in mammalian cells

Depositor

Dieter Wolf, Shashi Jain

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 _2-33 (NDUFS2 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 _2-33

Promoter

CMV

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 _2-78/myc-His

Plasmid

#127493

Purpose

Expresses myc-tagged _2-78 NDUFS2 in mammalian cells

Depositor

Dieter Wolf, Shashi Jain

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 _2-78 (NDUFS2 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 _2-78

Promoter

CMV

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pET-24d-Flag-SKP1

Plasmid

#213690

Purpose

Expresses human SKP1 in bacteria cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1 (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-T131D

Plasmid

#213600

Purpose

Expresses human SKP1 T131D mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-T131D (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-T131A

Plasmid

#213482

Purpose

Expresses human SKP1 T131A mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-T131A (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-T131E

Plasmid

#213601

Purpose

Expresses human SKP1 T131E mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-T131E (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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ReactionC_pBIG1a_ATG7_ATG10

Plasmid

#190860

Purpose

Plasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Inserts

ATG7 (ATG7 Human)
ATG10 (ATG10 )

Use

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mNG-eDHFR(69K6)-G-alpha-q(L254A)

Plasmid

#178851

Purpose

Mammalian expression of Gαq(L254A) fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-Gαq(L254A) (GNAQ Human)

Use

Tags

Expression

Mammalian

Mutation

Gαq: C9S/C10S/Q209L/L254A

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pETDuet-1_6xHis-TEV-mCh-NDP52

Plasmid

#187829

Purpose

Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NDP52 (CALCOCO2 Human)

Use

Tags

6xHisTag, TEV, and mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mNG-eDHFR(69K6)-G-alpha-q

Plasmid

#172106

Purpose

Mammalian expression of Gαq fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-Gαq (GNAQ Human)

Use

Tags

Expression

Mammalian

Mutation

Gαq: C9S/C10S/Q209L

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFV2-mVenus

Plasmid

#185653

Purpose

NDUFV2 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFV2 (NDUFV2 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 _34-78/myc-His

Plasmid

#127496

Purpose

Expresses myc-tagged _34-78 NDUFS2 in mammalian cells

Depositor

Dieter Wolf, Shashi Jain

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 _34-78 (NDUFS2 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 _34-78

Promoter

CMV

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc:Skp1(dC111):gs10:FRB:HA

Plasmid

#228532

Purpose

A fragment of human Skp1 protein in fusion with FRB domain through a flexible glycine-serine linker.

Depositor

Roman Jerala

Article

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

myc:Skp1(dC111):gs10:FRB:HA (SKP1 Human, Synthetic)

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-K142R

Plasmid

#213423

Purpose

Expresses human SKP1 K142R mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-K142R (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-97-101-5A

Plasmid

#213642

Purpose

Expresses human SKP1-97-101-5A mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1--97-101-5A (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-K163R

Plasmid

#213424

Purpose

Expresses human SKP1 K163R mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-K163R (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-PTPRQ(Trunc1)-COMP5AP-AviTag-9xHis

Plasmid

#157614

Purpose

Mammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PTPRQ (PTPRQ Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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