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InterCatch

Plasmid

#186906

Purpose

Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR and the other side expressing EGFP and puromycin resistance gene

Depositor

Allen Liu, Taekjip Ha

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

TfR-SpyCatcher003-sfCherry1-10

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-NFLAG TRF1

Plasmid

#16058

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

aa2-439

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pA5c-tesA-vhb50-8fadR

Plasmid

#86601

Purpose

expression of tesA and Vhb50 under the control of a LacUV5 promoter and fadR under the control of a pBAD promoter

Depositor

Yinjie Tang

Article

Liu et al Biotechnol Bioeng. 2016 Aug 6. doi: 10.1

Inserts

tesA
Vhb50
fadR

Use

Tags

Expression

Bacterial

Mutation

Promoter

LacUV5 and pBAD

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLPC-NFLAG TRF1 deltaM

Plasmid

#16064

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

Deleted myb domain (aa379-439)

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pLPC-NFLAG TRF1 deltaA

Plasmid

#16063

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

Deleted acidic domain (aa1-66)

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
CAG:: mCherry-2a-hM4Dnrxn

Plasmid

#52523

Purpose

Co-expresses hM4Dnrxn and a red fluorescent label

Depositor

Scott Sternson

Article

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Insert

mCherry-2a-hM4Dnrxn

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CAg

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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InterCatch-1xL

Plasmid

#186907

Purpose

Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through a single GGGGS linker and the other side expressing EGFP and puromycin resistance gene

Depositor

Allen Liu, Taekjip Ha

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

TfR-(GGGGS)1-SpyCatcher003-sfCherry1-10

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Hygro-NFLAG TRF1

Plasmid

#16277

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

aa2-439

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
CAG:: ChR2HA-2a-hM4D

Plasmid

#52520

Purpose

Co-expresses HA tagged channelrhodopsin and hM4D (Gi)

Depositor

Scott Sternson

Article

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Insert

ChR2-2a-hM4D

Use

Tags

2xHA on ChR2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

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InterTag-1xL

Plasmid

#186911

Purpose

Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a single GGGGS linker and the other side expressing BFP and puromycin resistance gene

Depositor

Allen Liu, Taekjip Ha

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

SpyTag003-sfCherry11-(GGGGS)1-PDGFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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SPLICS LY-MT Long P2A

Plasmid

#213613

Purpose

Detect the long-range Lysosome-Mitochondria contact

Depositor

Tito Calì

Article

Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

Insert

SPLICS LY-MT Long P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SPLICS LY-MT Short P2A

Plasmid

#213614

Purpose

Detect the short-range Lysosome-Mitochondria contact

Depositor

Tito Calì

Article

Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

Insert

SPLICS LY-MT Short P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPC-NFLAG TRF1 deltaA deltaM

Plasmid

#16065

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

Deleted acidic domain (aa1-66) and myb domain (aa…

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pWZL Hygro-NFLAG TRF1 deltaA

Plasmid

#16278

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Deleted acidic domain (aa1-66)

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pWZL Hygro-NFLAG TRF1 deltaM

Plasmid

#16281

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Deleted myb domain (aa379-439)

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pOTTC1328 - pAAV EF1a HA-hM3D(Gq)

Plasmid

#89149

Purpose

An AAV packaging plasmid that expresses HA-tagged hM3D(Gq) excitatory DREADD from the EF1a promoter.

Depositor

Brandon Harvey

Article

Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

Insert

hM3D(Gq)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC1329 - pAAV EF1a HA-hM4D(Gi)

Plasmid

#89150

Purpose

An AAV packaging plasmid that expresses HA-tagged hM4D(Gi) inhibitory DREADD from the EF1a promoter.

Depositor

Brandon Harvey

Article

Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

Insert

hM4D(Gi)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Hygro-NFLAG TRF1 deltaA deltaM

Plasmid

#16297

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Deleted acidic domain (aa1-66) and myb domain (aa…

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D

Plasmid

#52521

Purpose

Co-expresses HA tagged channelrhodopsin and hM4D (Gi) from a Cre-dependent virus

Depositor

Scott Sternson

Article

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Insert

ChR2-2a-hM4D

Use

AAV and Cre/Lox

Tags

2xHA on ChR2

Expression

Mutation

Promoter

CBA

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pcdna DSG-2 Tailless

Plasmid

#89466

Purpose

DSG-2 tailless control

Depositor

Daniel Conway

Article

Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

Insert

desmoglein-2 (DSG2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge