We narrowed to 721 results for: SG

1. pCIRCNpu

   Plasmid

   #74226

   Purpose

   Vector for SICLOPPS-mediated protein circularization using the Nostoc punctiforme DnaE intein.

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Npu DnaE C-intein and Npu DnaE N-intein

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lac

   Available since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-actin prom-dsRed-adra2a.1227.shRNA

   Plasmid

   #67880

   Purpose

   expresses dsRed and shRNA to knock down adra2a receptors

   Depositor

   William Wisden

   Article

   Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

   Insert

   dsRed and shRNA to knock down the mouse adra2 receptor
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chicken beta actin

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pMXs-IRES-blast 3xFlag-SPNS1(E164K)

   Plasmid

   #198569

   Purpose

   SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   changed glutamic acid E164 to lysine (E164K)

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-mitoX4-mCerulean

   Plasmid

   #185807

   Purpose

   Mitochondrial targetted (COX8) mCerulean

   Depositor

   Dipayan Chaudhuri

   Article

   Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

   Insert

   mito-mCerulean
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9-Puro HLAG-2A-sgRNA

   Plasmid

   #182551

   Purpose

   Cas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G gene

   Depositor

   Santiago Miriuka

   Article

   Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

   Insert

   hSpCas9-2A-Puro-HLAG-2A-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-4T-3-TRC8 513-664

   Plasmid

   #185352

   Purpose

   RING domain-containing GST fusion protein for ubiquitination assays.

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   TRC8 (RNF139 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 513-664 only

   Promoter

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET3a-UBE2J1 1-283

   Plasmid

   #185348

   Purpose

   In vitro transcription and translation in bacterial system

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   UBE2J1 (UBE2J1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Cytoplasmic domain only, aa 1-283

   Promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9-Puro HLAG-2B-sgRNA

   Plasmid

   #182552

   Purpose

   Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

   Depositor

   Santiago Miriuka

   Article

   Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

   Insert

   hSpCas9-2A-Puro-HLAG-2B-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_Donor/Reporter-COL4A5

   Plasmid

   #130280

   Purpose

   COL4A5 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A5 donor template

   Depositor

   Silvestro Conticello

   Article

   Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

   Insert

   mCherry-eGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Mouse GFP-PRMT7 LDIG(69-72)AAAA

    Plasmid

    #65044

    Purpose

    Expresses Catalytically inactive GFP-PRMT7 LDIG(69-72)AAAA mutant

    Depositor

    Steven Clarke

    Article

    Feng et al J Biol Chem. 2013 Dec 27;288(52):37010-2

    Insert

    PRMT7 (Prmt7 Mouse)
    

    Use

    Bacterial propagation

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    L69A D70A I71A G72A

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only