Addgene

We narrowed to 740 results for: SG

Showing: 1 - 20 of 740 results

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Sg-A

Plasmid

#83807

Purpose

Express sgRNA targeting SA site

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

SgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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EK0395 mCherry-SG(s70587)SG-EGFP (pEAQ)

Plasmid

#191170

Purpose

EK0285 in plant-expressable format: Sensor 1 (for EK0208); mCherry marker, EGFP output.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:s70587:EGFP (plant)

Use

Tags

Expression

Plant

Mutation

WT

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-AAVS1-sg

Plasmid

#194721

Purpose

px458 with guide RNA that target hAAVS1

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

Cas9 (AAVS1 Streptococcus pyogenes)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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SG-3'REL

Plasmid

#27257

Depositor

Thomas Gilmore

Article

Starczynowski et al Oncogene. 2003 Oct 9. 22(44):6928-36.

Insert

REL (REL Human)

Use

Tags

GAL4

Expression

Mammalian

Mutation

Contains aa 278-587 of REL

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only
pBT120a17-sg-del

Plasmid

#214347

Purpose

Expresses pIII

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

gIII-xLuxAB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX458-MAFB-sg

Plasmid

#194720

Purpose

px458 with guide RNA that target hMAFB

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

Cas9 (MAFB Streptococcus pyogenes)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-sg-1

Plasmid

#190607

Purpose

Expresses a gRNA for base editing the EGFP Kozak sequence of pWPT-/mEGFP-1T-IRES-mCherry

Depositor

Alessandro Quattrone

Article

Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

Insert

gRNA sequence

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-mGreenLantern332

Plasmid

#188483

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern fluorescent protein site 332.

Depositor

Johan Sosa, Indeever Madireddy

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern sgRNA

Use

CRISPR

Tags

PB_rtTA_BsmBI

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-CMV152

Plasmid

#188485

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 152.

Depositor

Johan Sosa, Indeever Madireddy

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-CMV162

Plasmid

#188486

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 162.

Depositor

Johan Sosa, Indeever Madireddy

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-CMV51

Plasmid

#188484

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 51.

Depositor

Johan Sosa, Indeever Madireddy

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sg-CMV152

Plasmid

#179916

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 152.

Depositor

Johan Sosa, Indeever Madireddy

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sg-CMV51

Plasmid

#179915

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 51.

Depositor

Johan Sosa, Indeever Madireddy

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sg-CMV162

Plasmid

#179917

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 162.

Depositor

Johan Sosa, Indeever Madireddy

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sg-mGreenLantern332

Plasmid

#179914

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern fluorescent protein site 332.

Depositor

Johan Sosa, Indeever Madireddy

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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EK0285 SFFV-mCherry-SG(s70587)SG-EGFP (FLP-IN)

Plasmid

#191140

Purpose

Expression of Sensor 1 (for EK0208) in mammalian cells; mCherry marker, EGFP output.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:s70587:EGFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0303 SFFV-mCherry-SG(Bdnf.s3)SG-EGFP (FLP-IN)

Plasmid

#191180

Purpose

Negative control sensor in human cells.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:Bdnf.s3:EGFP (negative control)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0301 SFFV-mCherry-SG(Bdnf.s1)SG-EGFP (FLP-IN)

Plasmid

#191144

Purpose

Expression of Sensor for murine Bdnf 3' UTR in mammalian cells; mCherry marker, EGFP output; 90 bp long

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:Bdnf.s1:EGFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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NSK024 SFFV-mCherry-SG(p53R248Q.s2)SG-EGFP (FLP-IN)

Plasmid

#191169

Purpose

Expression of a sensor for p53 mutant R248Q in mammalian cells; mCherry marker, EGFP output

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:p53(R248Q).s2:EGFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0325 SFFV-mCherry-SG(EGFP.s2)SG-Cre (FLP-IN)

Plasmid

#191160

Purpose

Expression of a sensor for EGFP CDS in mammalian cells; mCherry marker, Cre output

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:EGFP.s2:Cre

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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