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Showing: 1771 - 1800 of 6395 results

  13. pGAL1-VP1 (LEU2)

    Plasmid
    #182480
    Purpose
    Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains leucine auxotrophic marker (K. lactis LEU2).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Insert
    deltaVP1
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182480-plasmid-map-sequence-id-366129

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  14. mRuby3 only VLP

    Plasmid
    #182429
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and mRuby3 tagged with VP2C (GAL10 promoter). mRuby3 was codon-optimised for E. coli. Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-mRuby3
    deltaVP1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182429-plasmid-map-sequence-id-360111

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  17. pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

    Plasmid
    #176573
    Purpose
    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:
    Insert
    Dispersin B
    Use
    Tags
    Avitag, Hexahistidine tag, and mEGFP
    Expression
    Bacterial
    Mutation
    E184Q
    Promoter
    T7
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    176573-plasmid-map-sequence-id-348271

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  18. pET29b-mVenus-dspB(E184Q W330Y)-6xHis

    Plasmid
    #176577
    Purpose
    Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:
    Insert
    Dispersin B
    Use
    Tags
    Hexahistidine tag and mVenus
    Expression
    Bacterial
    Mutation
    E184Q W330Y
    Promoter
    T7
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    176577-plasmid-map-sequence-id-348269

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  19. pCfB6637

    Plasmid
    #106164
    Purpose
    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6681 into Yarrowia lipolytica chromosomal location IntE_3, amp resistance
    Depositor
    Irina Borodina
    Article
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Insert
    unknown
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106164-plasmid-map-sequence-id-206244

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  27. pLz37_CB_operon_CsoS2_N1-N4

    Plasmid
    #140856
    Purpose
    Halothiobacillus neapolitanus carboxysome operon with full length CsoS2. For heterologous expression of carboxysomes in E. coli.
    Depositor
    David Savage
    Article
    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-
    Insert
    CbbL, CbbS, CsoS2 (full-length), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    140856-plasmid-map-sequence-id-300524

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  30. pET28a-λN-βLac

    Plasmid
    #162039
    Purpose
    Expresion of the fusion protein of the λN domain of the λ phage and ampR gene (encoding the enzyme β-lactamase without the native signal sequence)
    Depositor
    Karen Polizzi
    Article
    Moya Ramirez et al Biosens Bioelectron. 2019 Jul 15;137:199
    Insert
    λN domain of the λ phage and a β-lactamase fusion protein
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    162039-plasmid-map-sequence-id-325526

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Showing: 1771 - 1800 of 6395 results