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p2T-U6-sgGAA

Plasmid

#232724

Purpose

Cas9 guide RNA targeting GAA repeats for A-to-G base editing

Depositor

David Liu

Article

Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

Insert

SpCas9 gRNA targeting GAA repeats

Expression

Mammalian

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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Dom neg CstF64

Plasmid

#127250

Purpose

Expresses the Dominant Negative CstF64 in mammalian cells; DN blocks polyadenylation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

CstF64 delta 282 (CSTF2 Human)

Use

Other

Expression

Mammalian

Mutation

insert @SanD1:GTCCAGGCGCCTACCCATACGACGTCCCAGACTAC…

Promoter

pEF1a

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-Nkx2-1*/Neo

Plasmid

#31272

Depositor

Tyler Jacks, Monte Winslow

Article

Winslow et al Nature. 2011 May 5. 473(7345):101-4.

Insert

Nkx2-1* (Nkx2-1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

A shNkx2-1 insensitive Nkx2-1 cDNA was created by…

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-NRAS

Plasmid

#52728

Purpose

retroviral expression of human NRAS

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

NRAS (NRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
pMXs-hs-3xHA-NHL only-LIN-41

Plasmid

#52723

Purpose

retroviral expression of human LIN-41 / TRIM71 containing only the NHL repeats and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

NHL-only contains only an initiating methionine a…

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-3xHA-7CtoA-LIN-41

Plasmid

#52726

Purpose

retroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations of all 7 cysteines in the RING domain and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

changed cysteines 12, 15, 61, 66, 69, 91, and 94 …

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-IRSp53(trunc N1)

Plasmid

#221272

Purpose

Expression of IRSp53 N-terminal truncation 1 (257-375) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 N-terminal truncation 1 (257-375) (Baiap2 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO_HSV.NWS.Ollas_mCherry-NLS

Plasmid

#178272

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

HSV.NWS.Ollas and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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frameshift ELL2

Plasmid

#127257

Purpose

For in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

XhoI site between the 1st &2nd Met was filled…

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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II-ATCC

Plasmid

#43272

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

II-ATCC

Use

Bacterial cloning

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
pDONR221-TMEM165

Plasmid

#132272

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

TMEM165 (TMEM165 Human)

Expression

Mammalian

Available Since

Jan. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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p2T-CMV-ABE8e_dNRCH-BlastR

Plasmid

#232720

Purpose

A-to-G base editor

Depositor

David Liu

Article

Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

Insert

ABE8e-dCas9(NRCH)

Expression

Mammalian

Mutation

within Cas9 D10A H840A

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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pHLSec2-irisin-his

Plasmid

#122729

Purpose

Expresses in mammalian cells (HEK293):glycosylated and secreted irisin-his

Depositor

Harold Erickson

Article

Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

Insert

irisin (ectodomain of FNDC5) (Fndc5 Mouse, Human, Bovine, Rat)

Tags

his and signal sequence for secretion

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro CXCR4 siRNA-2

Plasmid

#12272

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

CXCR4 siRNA (CXCR4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 ORF3B

Plasmid

#141272

Purpose

Gateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF3B

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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GPR182-DuET

Plasmid

#213272

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

GPR182 (GPR182 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 JN.1.11.1

Plasmid

#223272

Purpose

Encodes SARS-CoV-2 spike JN.1.11.1 for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike JN.1.11.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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WT USP7 TRAF

Plasmid

#227226

Purpose

Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Article

Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

aa 62-205

Promoter

T7-promoter

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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PLL5.0-Anillin ShRNA-GFP-AnillinFL

Plasmid

#187272

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full length

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

EGFP

Promoter

U6, CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-DIO-eGFP

Plasmid

#227237

Purpose

Large capacity AAV that expresses eGFP in neurons expressing Cre recombinase

Depositor

Richard Huganir

Article

Hong et al Nature. 2024 Nov;635(8038):398-405. doi:

Insert

eGFP (eGFP )

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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