We narrowed to 2,903 results for: BFP

1. lentiCRISPRv2-sgRNAs-del-CDK5RAP2-SVA-BFP

   Plasmid

   #202824

   Purpose

   Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CDK5RAP2 gene with EF-1apha for Cas9 and BFP expression

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   two sgRNAs (each with a U6 promoter) to delete SVA within CDK5RAP2 gene
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6 - two

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

   Plasmid

   #128755

   Purpose

   Expresses CYRANO gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   CYRANO sgRNA (OIP5-AS1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcg 2.0-BFP-circ-arRNA backbone

   Plasmid

   #213965

   Purpose

   pcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences o

   Depositor

   Wensheng Wei

   Article

   Yi et al Nat Biotechnol. 2022 Jun;40(6):946-955.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-AID-PcrA-UGI-T2A-mTagBFP2

   Plasmid

   #221224

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase with mTagBFP2 reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-PcrA-UGI-T2A-mTagBFP2
   

   Expression

   Mammalian

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-anti-GFP/BFP(LaG2) PAGER(FL)

   Plasmid

   #230022

   Purpose

   Expresses anti-GFP/BFP PAGER(FL) in mammalian cells

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   pAAV-MT3-LaG2-TEVcs-ALFA-hM4DxcpGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)

   Plasmid

   #219687

   Purpose

   Level 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

   Depositor

   Jennifer Nemhauser

   Insert

   T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
   

   Expression

   Plant

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. G417A_pLenti-Tet-BattP(GA)-BFP-2A-iCasp9-2A-Blast_rtTA3

   Plasmid

   #200631

   Purpose

   Negative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GA) landing pad

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

   Insert

   iCasp9
   

   Use

   Lentiviral

   Promoter

   pTRE3G

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRIP-SFFV-mtagBFP-2A STING V155M 368STOP

   Plasmid

   #102596

   Purpose

   Lentivector to express STING V155M delta 368 and mTagBFP

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   V155M delta 368

   Promoter

   SFFV

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRIP-SFFV-mtagBFP-2A STING-V155M S366A

   Plasmid

   #102593

   Purpose

   Lentivector to express STING V155M S366A and mTagBFP

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   V155M S366A

   Promoter

   SFFV

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

    Plasmid

    #171090

    Purpose

    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

    Inserts

    Sth1 dCas9
    Chloramphenicol acetyltransferase
    TagBFP
    

    Use

    CRISPR; Toxoplasma gondii expression

    Tags

    3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…

    Mutation

    D9A, H599A

    Promoter

    TUB1 (Toxoplasma gondii), transcriptionally linke…

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

    Plasmid

    #128749

    Purpose

    Expresses control gRNA

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. EK0211 CMV-TO-BFPmut-stop-t17d9b (FLP-IN)

    Plasmid

    #191139

    Purpose

    Expression of Trigger 2 in 3' UTR of an inactive BFP in mammalian cells. Used as second input in OR, AND gates

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    t17d9b
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-VTKS2-Dreadd-hM3Dq-HA-P2A-tBFP

    Plasmid

    #178707

    Purpose

    AAV vector for Cre-dependent transgene expression of Dreadd-hM3Dq-HA-P2A-tBFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Insert

    Dreadd-hM3Dq-HA-P2A-tBFP
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSCGB3A2_HL-P2A-TagBFP-PGK-NeoR-SCGB3A2_HR

    Plasmid

    #126697

    Purpose

    donor vector for targeting a 2A peptide followed by blue fluorescent reporter to the human SCGB3A2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

    Insert

    TagBFP
    

    Expression

    Mammalian

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. LV anti-FITC-LaG16-Y186F-T2A-BFP

    Plasmid

    #194931

    Purpose

    LV construct encoding Y186F mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y186F)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP

    Plasmid

    #31477

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV HA NLS Y2 T2A mTagBFP
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Mutation

    F13Y + S111Y on I-AniI = Y2 "reo" (res…

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. JDW 827 (pCS2-V5-mTagBFP2-hsKRAS4A-G12D)

    Plasmid

    #156409

    Purpose

    pCS2 CMV expression vector driving V5-tagged mTagBFP2 fused human KRAS4A-G12D

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12D (KRAS Human)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    mutant KRAS4A-G12D

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-TagBFP-P2A-Pre-SPHERE-SF-iGluSnFR

    Plasmid

    #187089

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Pre-SPHERE-SF-iGluSnFR
    

    Tags

    TagBFP-P2A

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. bactin-loxp-BFP-loxp-dsred-pBSII

    Plasmid

    #68972

    Purpose

    for transgenic animals

    Depositor

    David Traver

    Article

    Kobayashi et al Nature. 2014 Aug 21;512(7514):319-23. do

    Inserts

    lox-BFP-loxp
    dsred
    

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1-TagBFP-P2A-Pre-SPHERE-iGABASnFR

    Plasmid

    #187091

    Purpose

    GABA sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Pre-SPHERE-iGABASnFR
    

    Tags

    TagBFP-P2A

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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