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We narrowed to 2,015 results for: D10

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Showing: 1181 - 1200 of 2015 results

  4. pLV-EF1A-CD19.8H.8TM.BB.28.3z

    Plasmid
    #200673
    Purpose
    Modular CD8hinge-CD8transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production
    Depositor
    Michele Bernasconi
    Article
    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):
    Insert
    FMC63-CD8HD-CD8TM-41BB-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    Use
    Lentiviral
    Tags
    MycTag
    Expression
    Mammalian
    Promoter
    EF1a-short
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    200673-plasmid-map-sequence-id-391864

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  5. pD649-HAsp-SEMA4A-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156938
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    SEMA4A (SEMA4A Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156938-plasmid-map-sequence-id-389769

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  6. dCas9-GCN5 core

    Plasmid
    #179551
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179551-plasmid-map-sequence-id-354777

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  7. EFS-SpdCas9-Dnmt3A/3L-WT

    Plasmid
    #222497
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222497-plasmid-map-sequence-id-441134

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  11. pGBW-m4095420

    Plasmid
    #149523
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 nsp3
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    d1-745 d1063-1945
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149523-plasmid-map-sequence-id-297203

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  12. pCMV-T7-TadCBEd-SpG-P2A-EGFP (BKS777/AHK571)

    Plasmid
    #223124
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd-SpG with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadCBEd-SpG-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpG=D10A/D1135L/S1136W/G1218…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223124-plasmid-map-sequence-id-447705

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  13. pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187948
    Purpose
    ER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    ER50-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Mutation
    6 mutations, T371A, L384M, M421G, G521R, Y537S, N…
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187948-plasmid-map-sequence-id-373701

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  14. pCMV-T7-ABE8.20m-SpCas9-NRCH-P2A-EGFP (RMD55)

    Plasmid
    #197501
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRCH-BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.20 mutations in TadA and nSpCas9-NRCH(D10A/I…
    Promoter
    CMV and T7
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197501-plasmid-map-sequence-id-386690

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  15. pcDNA-dCas9-p300 Core (1645/1646 RR/EE)

    Plasmid
    #61359
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61359-plasmid-map-sequence-id-99792

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Showing: 1181 - 1200 of 2015 results