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pAB042b

Plasmid

#79193

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-MBPoff7

Expression

Bacterial

Promoter

Plac62; Ptet

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAB042d

Plasmid

#79194

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-p38_2_3

Expression

Bacterial

Promoter

Plac62; Ptet

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pFL-TK-AID3'UTR-MUT

Plasmid

#19987

Depositor

Nina Papavasiliou

Article

Teng et al Immunity. 2008 May . 28(5):621-9.

Insert

AID 3' UTR (Aicda Mouse)

Expression

Mammalian

Mutation

Mutation

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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Mad GSK3

Plasmid

#22995

Depositor

Edward De Robertis

Article

Eivers et al PLoS One. 2009 . 4(8):e6543.

Insert

Mad (Mad Fly)

Tags

FLAG

Expression

Mammalian

Mutation

S208A, S204A

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
Mad MM

Plasmid

#22997

Depositor

Edward De Robertis

Article

Eivers et al PLoS One. 2009 . 4(8):e6543.

Insert

Mad (Mad Fly)

Tags

FLAG

Expression

Mammalian

Mutation

S212A

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pAB043a

Plasmid

#79222

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ lambda cI-MBP; PlacIq lacI

Expression

Bacterial

Promoter

PlacZ; PlacIq

Available Since

April 15, 2017

Availability

Academic Institutions and Nonprofits only

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pAB082f

Plasmid

#79238

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-Cry1Ac-d23A

Expression

Bacterial

Promoter

Ppro1

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-GBKT7

Plasmid

#248655

Purpose

Empty vector for generating yeast 2-hybrid GAL4 DNA binding domain fusion constructs by Gateway cloning. It may also be used as an empty vector experimental control.

Depositor

Sarah Assmann, John Doonan

Article

Rossignol et al J Cell Sci. 2007 Oct 15;120(Pt 20):3678-

Type

Empty backbone

Use

Yeast 2-hybrid

Tags

GAL4 binding domain, Myc

Expression

Yeast

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST-GADT7

Plasmid

#248657

Purpose

Empty vector for generating yeast 2-hybrid GAL4 activation domain fusion constructs by Gateway cloning. It may also be used as an empty vector experimental control.

Depositor

Sarah Assmann, John Doonan

Article

Rossignol et al J Cell Sci. 2007 Oct 15;120(Pt 20):3678-

Type

Empty backbone

Use

Yeast 2-hybrid

Tags

GAL4 activation domain, HA

Expression

Yeast

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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L1p6_barley_sgRNA_scaffold

Plasmid

#231033

Purpose

Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 6

Depositor

Wilma van Esse

Article

Winkelmolen et al Plant Physiol. 2025 Aug 4;198(4):kiaf309

Insert

Scaffold for Cas9 mediated CRISPR

Use

CRISPR

Expression

Bacterial

Promoter

TaU6

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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L1p5_barley_sgRNA_scaffold

Plasmid

#231032

Purpose

Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 5

Depositor

Wilma van Esse

Article

Winkelmolen et al Plant Physiol. 2025 Aug 4;198(4):kiaf309

Insert

Scaffold for Cas9 mediated CRISPR

Use

CRISPR

Expression

Bacterial

Promoter

TaU6

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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L1p4_barley_sgRNA_scaffold

Plasmid

#231031

Purpose

Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 4

Depositor

Wilma van Esse

Article

Winkelmolen et al Plant Physiol. 2025 Aug 4;198(4):kiaf309

Insert

Scaffold for Cas9 mediated CRISPR

Use

CRISPR

Expression

Bacterial

Promoter

TaU6

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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L1p3_barley_sgRNA_scaffold

Plasmid

#231030

Purpose

Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 3

Depositor

Wilma van Esse

Article

Winkelmolen et al Plant Physiol. 2025 Aug 4;198(4):kiaf309

Insert

Scaffold for Cas9 mediated CRISPR

Use

CRISPR

Expression

Bacterial

Promoter

TaU6

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-C+F-ArchT-GFP

Plasmid

#209700

Purpose

Expresses ArchT-GFP dependent on the combined activity of CRE- and FLP-recombinases

Depositor

Peer Wulff

Article

Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

Insert

ArchT-GFP

Use

AAV

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBC135

Plasmid

#202262

Purpose

Level 2 for strain-specific barcode DNA tag with bc162 through Tn7 bacterial insertion, Kanamycin selectable marker and tagBFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc162

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC113

Plasmid

#202275

Purpose

Level 2 for strain-specific barcode DNA tag with bc140 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc140

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC115

Plasmid

#202277

Purpose

Level 2 for strain-specific barcode DNA tag with bc142 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc142

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC117

Plasmid

#202278

Purpose

Level 2 for strain-specific barcode DNA tag with bc144 through Tn7 bacterial insertion, Tetracyline selectable marker and GFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc144

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC118

Plasmid

#202279

Purpose

Level 2 for strain-specific barcode DNA tag with bc145 through Tn7 bacterial insertion, Tetracyline selectable marker and GFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc145

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC119

Plasmid

#202280

Purpose

Level 2 for strain-specific barcode DNA tag with bc146 through Tn7 bacterial insertion, Tetracyline selectable marker and GFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc146

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge