We narrowed to 6,098 results for: tTA

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pDest17_Gcn4 SATtoG KtoR

Plasmid

#231861

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 SATtoG KtoR

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 SATtoG KtoR

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Salro-Sox-like

Plasmid

#231531

Purpose

Expresses Salpingoeca rosetta full-length Sox-like in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Sox-like

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 ILVtoED solvvol

Plasmid

#231858

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED solvvol

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED solvvol

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

T7

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pDY2259 hNOLC1 atgRNA Paired Guide 1

Plasmid

#220989

Purpose

hNOLC1 atgRNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

NOLC1 atgRNA (NOLC1 Human)

Use

CRISPR

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDY2260 hNOLC1 atgRNA Paired Guide 2

Plasmid

#220990

Purpose

hNOLC1 atgRNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

NOLC1 atgRNA (NOLC1 Human)

Use

CRISPR

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDY2261 hACTB atgRNA Paired Guide 1

Plasmid

#220991

Purpose

hACTB atgRNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

ACTB atgRNA (ACTB Human)

Use

CRISPR

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDY2262 hACTB atgRNA Paired Guide 2

Plasmid

#220992

Purpose

hACTB atgRNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

ACTB atgRNA (ACTB Human)

Use

CRISPR

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-hL1-gRNA2-EGFP

Plasmid

#226003

Purpose

CRISPRi-KD of human LINE1

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

sgRNA targeting human L1HS

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA^EcTyr

Plasmid

#218766

Purpose

encodes EcTyrRS-VSMA* and EcTyr-tRNA-TAG

Depositor

Abhishek Chatterjee

Article

Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

Insert

EcTyrRS-VSMA*

Expression

Bacterial

Mutation

Y37V, D167G, D182S, F183M, L186A, D265R

Promoter

tacI

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-GFP-SPOP (R354H)

Plasmid

#197010

Purpose

Mammalian inducible expression vector for GFP-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

GFP

Expression

Mammalian

Mutation

R354H

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-GFP-SPOP (R221C)

Plasmid

#197009

Purpose

Mammalian inducible expression vector for GFP-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

GFP

Expression

Mammalian

Mutation

R221C

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV R354H SPOP 28-359

Plasmid

#197011

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Human)

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

R354H

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-SPOP-myc (R121Q)

Plasmid

#197007

Purpose

Mammalian inducible expression vector for myc-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

myc

Expression

Mammalian

Mutation

R121Q

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-SPOP-myc (R354H)

Plasmid

#197006

Purpose

Mammalian inducible expression vector for myc-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

myc

Expression

Mammalian

Mutation

R354H

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-SPOP-myc (R221C)

Plasmid

#197005

Purpose

Mammalian inducible expression vector for myc-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

myc

Expression

Mammalian

Mutation

R221C

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-SPOP-myc (BACK)

Plasmid

#197021

Purpose

Mammalian inducible expression vector for myc-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

myc

Expression

Mammalian

Mutation

Y353E

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSBK.011

Plasmid

#187211

Purpose

Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 3 [CTG-CAG]), and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35 (long a1/a2, barcode 3 [CTG-CAG])
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7/Lac

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSBK.012

Plasmid

#187212

Purpose

Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 4 [GTG-CAC]), and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35 (long a1/a2, barcode 4 [GTG-CAC])
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7/Lac

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSBK.015

Plasmid

#187216

Purpose

Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 7 [GAG-CTC]), and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35 (long a1/a2, barcode 7 [GAG-CTC])
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7/Lac

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSBK.016

Plasmid

#187217

Purpose

Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 8 [GCT-TGC]), and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35 (long a1/a2, barcode 8 [GCA-TGC])
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7/Lac

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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