We narrowed to 183 results for: tTA

...other viral tTA plasmids. tTA Viviana Gradinaru 104102 pCAG-tTA Mammalian expression of tTA from the CAG...Plasmid #27106 for rtTA. tTA Edward Hsiao 99118 pAAV-CAG-tTA AAV Tet-Off vector, expresses tTA from the CAG ...for Tet-Off tTA-Advanced Takeshi Imai 104109 pAAV-Syn1-tTA AAV Tet-Off vector, expressed tTA from the hSyn...Xu 26803 pEnt L1L3 EF1a-tTA-2 Gateway entry vector to express tTA from EF1α promoter, for Tet-Off. See...depending on whether they are used alongside a tTA or rtTA, respectively. You should also choose between...affinity to TetR and derivatives like rTetR, rtTA, and tTA. Dox has good tissue distribution, low toxicity...Bert Vogelstein Return to Top Transactivators (tTA or rtTA) Find a construct that expresses the transactivator...

...99118 pAAV-CAG-tTA CAG-driven, constitutive Expression of the tet-off transactivator (tTA). 2 Viviana Gradinaru...-ihSyn1-tTA Inducible Synapsin promoter (ihSyn) Expression of the tet-off transactivator (tTA) with a ... loop for amplified tTA expression. 1 Viviana Gradinaru 99121 pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible... tet-off transactivator (tTA) with a positive feedback loop for amplified tTA expression. 1 Viviana Gradinaru...

...non-standard), expressing Rep2 and PHP.eB Cap controlled by tTA-TRE amplification system Viviana Gradinaru 103006...non-standard), expressing Rep2 and PHP.S Cap controlled by tTA-TRE amplification system Viviana Gradinaru 127847...non-standard), expressing Rep2 and PHP.V1 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 175004...non-standard), expressing Rep2 and B10 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 175005...non-standard), expressing Rep2 and B22 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 185136...), expressing Rep2 and MaCPNS1 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 185137...), expressing Rep2 and MaCPNS2 Cap controlled by tTA-TRE amplification system Viviana Gradinaru All Available...

...levels of XFPs - Reduce the amount of tTA. Paradoxically, too much tTA leads to a reduced expression level...transcription. It is critical to express a minimal amount of tTA to achieve highest expression levels. Clearing large...

...March.  Addgene is now offering an AAV2 CAG-driven tTA expresser from the Gradinaru lab. This is useful ...AAV8 Optogenetics Boyden New serotypes pAAV-CAG-tTA AAV2 Molecular Tools Gradinaru New viral service ...

...pPRIME-Tet-GFP-FF3) and one with either tTA or rtTA (FUW-M2rtTA). In this kind of approach, a population...contrast, in the Tet-on system, tTA has been modified (and renamed rtTA) to bind TRE only in presence of...vector systems containing both your transgene and tTA/rtTA (pINDUCER20, pINDUCER21) have been developed, ...Doxycycline (Dox), the Tet-controlled transactivator (tTA, also expressed by your transfer vector) binds to...population of cells might express only the tTA or your transgene resulting in low inducibility and potentially...

...Gordon Fishell 99120-AAV1 pAAV-ihSyn1-tTA An AAV genome that expresses tTA from the hSyn1 promoter that contains...amplifed expression of the tTA Viviana Gradinaru 99121-AAV1 pAAV-ihSyn1-DIO-tTA An AAV genome with Cre-dependent...-dependent expression of the tTA from the hSyn1 promoter. Contains a positive feedback loop for amplifed...amplifed expression of the tTA Viviana Gradinaru 99130-AAV1 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses...Gradinaru 100798-AAV1 pAAV-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing; to be coinjected...to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA Ian Wickersham 104052-AAVPHP.V1 pAAV-CAG-DIO-EYFP...

... Name 99120  AAV1 pAAV-ihSyn1-tTA 99121  AAV1 pAAV-ihSyn1-DIO-tTA Viral vectors coming soon! These...

...created the tetracycline-controlled transactivator (tTA). They fused TetR with the transcriptional activation...domain VP16 taken from the herpes simplex virus. tTA binds to promoters containing TetO elements (often...tetracycline or one of its derivatives is added, it binds tTA, resulting in a confirmation change that prevents...

...control of an inducer, i.e. tet-off transactivator (tTA), is injected at a high dose to maximize the number...high (figure 4e) and low doses (figure 4f) of the tTA inducer virus, but the desired sparsity of labeling...

...Ian Wickersham 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used to complement deletion-mutant...

....), and tetracycline drug induction systems (ttA or rtTA) are between 1 to 2 kb in length. There are plenty...

...MATR3 Flag CMV ALS Yossi Shiloh 34927 pTRE_tau-LacZ::tTA-H100Y MAPT LacZ TRE Parkinson's, FTD Mark Mayford...HNRNPA1 His T7 ALS Tanja Mittag 169714 A1-LCD deltahexa HNRNPA1 His T7 ALS Tanja Mittag 169715 A1-LCD Aro- ...HNRNPA1 His T7 ALS Tanja Mittag 169716 A1-LCD Aro-- HNRNPA1 His T7 ALS Tanja Mittag 169717 A1-LCD Aro+ HNRNPA1...HNRNPA1 His T7 ALS Tanja Mittag 169718 A1-LCD Perfect Mix HNRNPA1 His T7 ALS Tanja Mittag 169719 A1-LCD Patchy...HNRNPA1 His T7 ALS Tanja Mittag 178659 A1-LCD-12F+12Y HNRNPA1 His T7 ALS Tanja Mittag 178660 A1-LCD+7F-7Y ...HNRNPA1 His T7 ALS Tanja Mittag 178661 A1-LCD-9F+6Y HNRNPA1 His T7 ALS Tanja Mittag 178662 A1-LCD-8F+4Y HNRNPA1...HNRNPA1 His T7 ALS Tanja Mittag 178663 A1-LCD-9F+3Y HNRNPA1 His T7 ALS Tanja Mittag 178664 A1-LCD-10R HNRNPA1...

...with tTALE to “stain” DNA. TALE proteins can bind specific DNA sequences which brings the attached fluorescent...They created plasmids for purification of tTALE-eGFP and tTALE-mCherry. In comparison to intercalating ...Tsang Figure 4: Double stranded DNA bound by tTALE-FP with the fluorescent protein fluorescing green...high salt conditions - up to 100 mM NaCl. These tTALE-FPs also have higher DNA binding affinity for AT-rich...stained evenly in 1x TE buffer. The lab also used tTALE-FP in a single molecule-fluorescence assay to monitor...molecules in real-time. Find plasmids expressing tTALE-FP Shin et al., Sci. Rep. 2019. https://doi.org/...

...with these new photoconvertible proteins. With Sattarzadeh et al.’s discovery that GFP itself is photoconvertible...GFP-labeled plant cells at 405 nm instead of 488 nm, Sattarzadeh et al. noticed that GFP had turned red. They ... cytosol via cytoplasmic strands. Image from Sattarzadeh et al., Sci Rep. 2015 CC BY 4.0 Applications ... photoconversion signal may not be observed. Sattarzadeh et al. found that only the protonated form of...appropriate for high-pH cellular compartments. Sattarzadeh et al. have shown that GFP can be rapidly and...empty backbones containing GFP variants used by Sattarzadeh et al., as well as a wide variety of other fluorescent...refresher, see our FP blog posts.     References Sattarzadeh A, Saberianfar R, Zipfel WR, Menassa R, Hanson...

...endogenous molecular target, meaning the dye will be attached to the target through binding of the molecular...encoded tag is required. These dyes can also be attached to an antibody to detect various targets, such...or glycans. Fluorophores can also be covalently attached to self-labeling proteins. Some common examples...covalently reacts with a small substrate ligand motif attached to a fluorophore or other molecule. When paired...high specificity, increased versatility in probe attachment, and compatibility with far-red and near-infrared...spectrum.03690-23  Salari, M., Bitounis, D., Bhattacharya, K., Pyrgiotakis, G., Zhang, Z., Purington, ...

...bolster and modulate our immune defenses to better attack cancer. Combing our knowledge with our bodies’ ...concept of immunoediting, by way of favorably attaining the equilibrium stage. This can be called “stalemate...created by the cancer cells to shield themselves from attack. It is a region of incessant chemical activity,...that suppress APCs. T cells that would normally attack the cancer cells are also suppressed by chemical...microenvironment TGFβ, IL-10, can also help the immune system attack tumors. Attempts have been made to use whole cell...collaborative therapies must be explored. When attacking cancer, multiple immunotherapy targets can be ...

...(Length) Common Variants Cre loxP ATAACTTCGTATAgcatacatTATACGAAGTTAT 13 bp inverted repeats + 8 bp spacer... lox511, loxN, lox66/lox71 Dre rox TAACTTTAAATAATgccaATTATTTAAAGTTA 14 bp inverted repeats + 4 bp spacer...

...cofactor that in its active form is covalently attached to the active site of key metabolic enzymes including...Southern/Northern blotting Cell surface labelling Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated...Photobiotin acetate consists of a biotin molecule attached to a photoreactive arylazide group. Under strong...methods. Scientists have commonly used this method to attach proteins or polypeptides to a solid substrate (... & Cronan, J. E. “Molecular Biology of Biotin Attachment to Proteins; Symposium: Nutrition, Biochemistry...

...is no coming back. The whole lab is emotionally attached to this disease.” He says that the award from ...Patients affected by AHC suffer from recurrent attacks of unilateral paralysis (hemiplegia) that are often...often painful and accompanied by migraines. Those attacks can be brought on by a number of stressors, including...syndrome (WS4) by Pediatrician Duangrurdee Wattanasirichaigoon carried different mutations in a gene called...