Addgene

Showing: 1201 - 1220 of 1717 results

Results per page:

Sort results by:

pDGE651

Plasmid

#153229

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE652

Plasmid

#153230

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET41a iGluf

Plasmid

#119833

Purpose

Production of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluf

Use

Tags

GST

Expression

Bacterial

Mutation

E25D

Promoter

T7

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI syn iGluf

Plasmid

#106121

Purpose

Fast indicator for synaptic glutamate imaging (synapsin promoter)

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR E25D variant

Use

Tags

Myc and PDGFR

Expression

Mammalian

Mutation

Changed Glu 25 to Asp

Promoter

synapsin

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
UBQ10pro-nlsABACUS2-400n-NosT

Plasmid

#203728

Purpose

Binary vector for the transformation of the nlsABACUS2-400n biosensor for (+)-Abscisic acid into Arabidopsis

Depositor

Alexander Jones

Article

Rowe et al Nat Plants. 2023 Jun 26. doi: 10.1038/s4

Insert

nlsABACUS2-400n

Use

Synthetic Biology

Tags

Myc

Expression

Plant

Mutation

Promoter

AtUBQ10pro 1500bp

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV iGluu

Plasmid

#105832

Purpose

Fast indicator for extracellular glutamate imaging

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR S72T variant

Use

Tags

Myc and PDGFR

Expression

Mammalian

Mutation

Changed Ser 72 to Thr

Promoter

CMV

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV iGluf

Plasmid

#105833

Purpose

Fast indicator for extracellular glutamate imaging

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR E25D variant

Use

Tags

Myc and PDGFR

Expression

Mammalian

Mutation

Changed Glu 25 to Asp

Promoter

CMV

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMKO.1-puro/hFOXH1 shRNA1

Plasmid

#59303

Purpose

Knockdown of human FOXH1

Depositor

Shinya Yamanaka

Article

Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

Insert

hFOXH1 shRNA (FOXH1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pMXs-hFOXH1GR

Plasmid

#59311

Purpose

Forced expression of human FOXH1GR

Depositor

Shinya Yamanaka

Article

Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

Insert

FOXH1 (FOXH1 Human)

Use

Retroviral

Tags

cGR - hormone binding domain of glucocorticoid re…

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_N73_eMagBF_V5

Plasmid

#235642

Purpose

Encodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5. No nuclear export sequence to allow nuclear localization.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Use

Tags

eMagBF-V5

Expression

Mammalian

Mutation

residues 1-73

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_C74_eMagAF_V5_NES

Plasmid

#235636

Purpose

Encodes fusion protein of ALIBi C-terminal fragment (residues 74-321), eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence (order reversed)

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Use

Tags

eMagAF-V5-NES

Expression

Mammalian

Mutation

residues 74-321

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_N256_eMagBF_V5_NES

Plasmid

#235630

Purpose

Encodes ALIBi N-terminal fragment, residues 1-256, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Use

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-256

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_Fbl_N73_eMagBF_V5

Plasmid

#235643

Purpose

Encodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5, Fbl for targeting to nucleolus.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Use

Tags

Fbl-N73- and eMagBF-V5

Expression

Mammalian

Mutation

residues 1-73

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_NES_V5_eMagAF_C233

Plasmid

#235629

Purpose

Encodes ALIBi C-terminal fragment, residues 233-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Use

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 233-321

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_NES_V5_eMagAF_C141

Plasmid

#235621

Purpose

Encodes ALIBi C-terminal fragment, residues 141-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Use

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 141-321

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_NES_V5_eMagAF_C222

Plasmid

#235627

Purpose

Encodes ALIBi C-terminal fragment, residues 222-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Use

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 222-321

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_NES_V5_eMagAF_C196

Plasmid

#235625

Purpose

Encodes ALIBi C-terminal fragment, residues 196-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Use

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 196-321

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_N232_eMagBF_V5_NES

Plasmid

#235628

Purpose

Encodes ALIBi N-terminal fragment, residues 1-232, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Use

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-232

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_V5_eMagAF_C74_cMycNLS

Plasmid

#235640

Purpose

Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, cMyc nuclear localization sequence for targeting to nucleus

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Use

Tags

V5-eMagAF and cMycNLS

Expression

Mammalian

Mutation

residues 74-321

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_N140_eMagBF_V5_NES

Plasmid

#235620

Purpose

Encodes ALIBi N-terminal fragment, residues 1-140, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Use

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-140

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge