Addgene

We narrowed to 7,339 results for: Lif;

Showing: 1201 - 1220 of 7339 results

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12URA-B-ScTop2∆CTD

Plasmid

#214924

Purpose

Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

Depositor

James Berger

Article

Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

Insert

topoisomerase II

Use

Tags

6x His-TEV

Expression

Yeast

Mutation

Deleted amino acids 1178-1428

Promoter

Gal 1/10

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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eMagA-mCherry-Mito

Plasmid

#162251

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagA-mCherry-OMP25

Use

Tags

Expression

Mammalian

Mutation

Promoter

beta-actin promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pTrex-Luc-Neo

Plasmid

#48336

Purpose

Trasgenically expresses firefly luciferase in T. cruzi with neomycin selection

Depositor

Rick Tarleton

Article

Sanchez-Valdez et al Elife. 2018 Mar 26;7. pii: 34039. doi: 1

Insert

firely luciferase

Use

Trypanasoma cruzi expression

Tags

Expression

Mutation

Promoter

T. cruzi rRNA

Available since

July 20, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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siRNA-resistant mNG-ORP8

Plasmid

#195170

Purpose

mammalian expression of siRNA-resistant ORP8 tagged with mNeonGreen

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ORP8 (OSBPL8 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Wobble mutations for siRNA resistance from K174 t…

Promoter

CMV

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ER-GCaMP3

Plasmid

#64854

Purpose

Endoplasmic reticulum (ER)-targeted version of the Ca2+ indicator GCaMP3; targeted to cytosolic face of ER membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CYP450-GCaMP3

Use

Tags

N-terminal 27 amino acids from CYP450

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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N-WASP

Plasmid

#186132

Purpose

For expression of recombinant N-WASp in bacteria cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

WASL (WASL Rat)

Use

Tags

His6

Expression

Bacterial

Mutation

Amino Acid residues 183-193 fused to 273-501

Promoter

T7

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEBTet_NAD-Snifit-mito

Plasmid

#113916

Purpose

Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NAD-Snifit

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV TetO2

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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mNG-Mfn1

Plasmid

#195162

Purpose

mammalian expression of Mfn1 tagged with mNeonGreen

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

Mfn1 (MFN1 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pEBTet_NADP-Snifit-nucl

Plasmid

#113918

Purpose

Plasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV TetO2

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEBTet_NAD-Snifit-cyto

Plasmid

#113914

Purpose

Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NAD-Snifit

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV TetO2

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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SB_concave

Plasmid

#110102

Purpose

pBXPHM3 with scaffold Sybody of the concave library

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Insert

scaffold Sybody of the concave library

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

May 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-RT005-HaloGPM

Plasmid

#163385

Purpose

2nd generation lentiviral vector to express sHalotag-sGFP module in the G-baToN system

Depositor

Monte Winslow

Article

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

Halotag-GFP-PDGFRtm-2A-mCherry (HaloGPM)

Use

Lentiviral

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pFsynW SYP-GFP

Plasmid

#197283

Purpose

Encodes for viral expression of full-length wild type synaptophysin (SYP). Visualized by a C-terminal GFP.

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYP and GFP (Syp Rat)

Use

Lentiviral

Tags

1D4 epitope

Expression

Mutation

N/A

Promoter

hSyn (human synapsin I promoter)

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEBTet_NAD-Snifit-nucl

Plasmid

#113915

Purpose

Plasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NAD-Snifit

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV TetO2

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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SB_loop

Plasmid

#110103

Purpose

pBXPHM3 with scaffold Sybody of the loop library

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Insert

scaffold Sybody of the loop library

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

May 24, 2018

Availability

Academic Institutions and Nonprofits only

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AAV.hSyn.GLPLight-ctr

Plasmid

#187468

Purpose

Expresses GLPLight-ctr in mammalian cells

Depositor

Tommaso Patriarchi

Article

Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

Insert

GLPLight-ctr

Use

AAV

Tags

Flag tag

Expression

Mutation

Promoter

human Synapsin-1

Available since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mCherry-VPS4A

Plasmid

#115334

Purpose

expresses mCherry-VPS4A in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

VPS4A (VPS4A Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

silent mutation in position 4050 C to T

Promoter

CMV

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST_6xUAS:mNeonGreen-Rab5a

Plasmid

#192355

Purpose

zfRabaa tagged with mNeonGreen, expressed under the UAS promoter

Depositor

Francesca Peri

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

Rab5a (rab5aa Zebrafish)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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3xnls-G-Ca-FLITS

Plasmid

#191463

Purpose

Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

G-Ca-FLITS

Use

Tags

3xNLS

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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TagRFP-T-VAPB(1- 218)- eMagB

Plasmid

#162255

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

TagRFP-T-VAPB(1- 218)- eMagB

Use

Tags

Expression

Mammalian

Mutation

Promoter

beta-actin promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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