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ku70 insUP+pPGI+NAT+tNOS+ku70 insD (SBE217)

Plasmid

#195049

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pPGI for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pPGI

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pAct-AtINTBxb1

Plasmid

#127536

Purpose

Plasmid encodes A. thaliana codon optimized Integrase Bxb1.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase Bxb1 coding sequence codon optimized for A. thaliana expression.

Expression

Plant

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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ST-SBP-EGFP_puromycin

Plasmid

#65270

Purpose

ST reporter only ( no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated sialyltransferase (ST6GAL1 Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF5A-Rigor

Plasmid

#172202

Purpose

Expresses KIF5A G235A rigor mutant tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Farias et al Cell Rep. 2015 Nov 10;13(6):1221-1232. d

Insert

KIF5A (Kif5a Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

Changed Glycine 235 to Alanine

Promoter

CMV

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-F-API5

Plasmid

#157662

Purpose

Expresses API5 in mammalian cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Tags

FLAG tag

Expression

Mammalian

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pAct-AtINT4

Plasmid

#127530

Purpose

Plasmid encodes A. thaliana codon optimized Integrase 4.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 4 coding sequence codon optimized for A. thaliana expression.

Expression

Plant

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b-API5

Plasmid

#157655

Purpose

Expresses API5 in E.coli cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Tags

His tag

Expression

Bacterial

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-neo-(HA)3-ε

Plasmid

#198531

Purpose

Expression of HA-tagged AP-4 ε in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

Insert

AP-4 ε (AP4E1 Human)

Tags

triple HA tag

Expression

Mammalian

Mutation

silent substitutions in codons 235, 262, 905 and …

Promoter

CMV

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-Hook2

Plasmid

#198524

Purpose

Expression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

Insert

Hook2 (HOOK2 Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

His488Gln substitution

Promoter

ADH1

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409)

Plasmid

#133790

Purpose

CAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized NmeCas9

Tags

NLS(SV40)-3xFLAG

Expression

Mammalian

Mutation

n/a

Promoter

CAG

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pAct-AtINT2

Plasmid

#127529

Purpose

Plasmid encodes A. thaliana codon optimized Integrase 2.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 2 coding sequence codon optimized for A. thaliana expression.

Expression

Plant

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pAct-AtINT5

Plasmid

#127531

Purpose

Plasmid encodes A. thaliana codon optimized Integrase 5.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 5 coding sequence codon optimized for A. thaliana expression.

Expression

Plant

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pAct-AtINT7

Plasmid

#127532

Purpose

Plasmid encodes A. thaliana codon optimized Integrase 7.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 7 coding sequence codon optimized for A. thaliana expression.

Expression

Plant

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pAct-AtINT9

Plasmid

#127533

Purpose

Plasmid encodes A. thaliana codon optimized Integrase 9.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 9 coding sequence codon optimized for A. thaliana expression.

Expression

Plant

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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API5-CRISPR

Plasmid

#157664

Purpose

Expresses Cas9 and sgRNA targeting API5

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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5-HT2C_VNI

Plasmid

#252897

Purpose

Mammalian expression of RNA edited version of human 5-HT2C

Depositor

Cesare Orlandi

Article

Bonniwell et al ACS Chem Neurosci. 2025 Oct 1;16(19):389

Insert

5-HT2C (HTR2C Human)

Tags

HA tag, inserted after signal peptide

Expression

Mammalian

Mutation

VNI isoform

Available Since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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5-HT2C_VNV

Plasmid

#252885

Purpose

Mammalian expression of RNA edited version of human 5-HT2C

Depositor

Cesare Orlandi

Article

Bonniwell et al ACS Chem Neurosci. 2025 Oct 1;16(19):389

Insert

5-HT2C (HTR2C Human)

Tags

HA tag, inserted after signal peptide

Expression

Mammalian

Mutation

VNV isoform

Available Since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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5-HT2C_VGI

Plasmid

#252884

Purpose

Mammalian expression of RNA edited version of human 5-HT2C

Depositor

Cesare Orlandi

Article

Bonniwell et al ACS Chem Neurosci. 2025 Oct 1;16(19):389

Insert

5-HT2C (HTR2C Human)

Tags

HA tag, inserted after signal peptide

Expression

Mammalian

Mutation

VGI isoform

Available Since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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5-HT2C_INV

Plasmid

#252898

Purpose

Mammalian expression of RNA edited version of human 5-HT2C

Depositor

Cesare Orlandi

Article

Bonniwell et al ACS Chem Neurosci. 2025 Oct 1;16(19):389

Insert

5-HT2C (HTR2C Human)

Tags

HA tag, inserted after signal peptide

Expression

Mammalian

Mutation

INV isoform

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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5-HT2C_ISI

Plasmid

#252896

Purpose

Mammalian expression of RNA edited version of human 5-HT2C

Depositor

Cesare Orlandi

Article

Bonniwell et al ACS Chem Neurosci. 2025 Oct 1;16(19):389

Insert

5-HT2C (HTR2C Human)

Tags

HA tag, inserted after signal peptide

Expression

Mammalian

Mutation

ISI isoform

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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