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DCT.VV.Orf159

Plasmid

#12847

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf159

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
pUC19_CoV-2_ frD_Δ3E/VSVG Δ67a

Plasmid

#253172

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; VSV glycoprotein replaces orf3a and envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with VSV-G

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_ frD_Δ3E/N1 PR8 Δ67a

Plasmid

#253169

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a, envelope, orf6, orf7a; Influenza N1 (PR8) replaces orf3a and envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with Influenza N1 PR8

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_ frD_Δ3E/H1 PR8 Δ67a

Plasmid

#253168

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a, envelope, orf6, orf7a; Influenza H1 (PR8) replaces orf3a and envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with Influenza H1 PR8

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2_ΔrE/N1 PR8 Δ67 3*

Plasmid

#253150

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope (residual AA), orf6, orf7a;stop codon at AA213 in orf3a; Influenza N1 (PR8) replaces env

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with Influenza N1 PR8

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2 ΔE7 3* Δ6/H1 PR8

Plasmid

#253147

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; Influenza H1 (PR8) replaces orf6

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with Influenza H1 PR8

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2 ΔE7 3* Δ6/H1 WSN

Plasmid

#253146

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; Influenza H1 (WSN) replaces orf6

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with Influenza H1 WSN

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2_ΔrE/N1 WSN Δ67 3*

Plasmid

#253154

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of env (residual AA), orf6, orf7a; stop codon at AA213 in orf3a; Influenza N1 (WSN) replaces envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with Influenza N1 WSN

Expression

Bacterial

Available Since

May 6, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_Δ3E_RABVG Δ67a

Plasmid

#253177

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a, envelope, orf6, orf7a; rabies glycoprotein replaces orf3a and envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with RABVG

Expression

Bacterial

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD ΔrS_GP LCMV ΔE67 3*

Plasmid

#253178

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of spike (partial), envelope, orf6, orf7a;stop codon at AA213 in orf3a; LCMV glycoprotein replaces spike

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with LCMV GP

Expression

Bacterial

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_Δ3E_GP LCMV Δ67a

Plasmid

#253179

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a, envelope, orf6, orf7a; LCMV glycoprotein replaces orf3a and envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with LCMV GP

Expression

Bacterial

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pGenLenti murine CD300LF

Plasmid

#235668

Purpose

3rd Generation lentiviral vector for expression of murine CD300LF. Murine CD300LF is the receptor for murine norovirus, and expression confers susceptibility to this virus. Puromycin resistance.

Depositor

Edward Emmott

Article

Frazer J.T. et al bioRxiv 2026.03.19.712921; doi: 10.64898

Insert

Murine CD300LF (Cd300lf Mouse)

Use

Lentiviral

Promoter

CMV

Available Since

March 24, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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CVS-N2c-RVdG-MCP:oScarlet:KASH-6xMBS

Plasmid

#231011

Purpose

CVS N2c RVdG genome plasmid. Utilizes MS2 tagging to deliver the MCP:oScarlet:KASH transcript to the outer nuclear membrane. Insert high complexity barcode between SacII RE sites distal to 6xMBS site.

Depositor

Tomasz Nowakowski

Article

Shin et al bioRxiv [Preprint]. 2024 Dec 11:2024.10.

Insert

MCP-oScarlet-KASH-6xMBS-SacIIMCS

Use

Neurotropic virus

Tags

KASH and MCP

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pVS2-GhCLA

Plasmid

#234906

Purpose

This all-in-one vector is used to specifically silence the GhCLA gene via virus-induced gene silencing (VIGS).

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

VIGS fragement of GhCLA gene

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVS2-NbPDS

Plasmid

#234908

Purpose

This all-in-one vector is used to specifically silence the NbPDS gene via virus-induced gene silencing (VIGS).

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

VIGS fragement of NbPDS gene

Expression

Plant

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pVM2-GoPGF-HD1

Plasmid

#234909

Purpose

This all-in-one vector is used to specifically silence the GoPGF and HD1 gene via virus-induced gene silencing (VIGS), simultaneously.

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

VIGS fragements of GoPGF and GhHD1

Expression

Plant

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pVS2-GoPGF

Plasmid

#234907

Purpose

This all-in-one vector is used to specifically silence the GoPGF gene via virus-induced gene silencing (VIGS).

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

VIGS fragement of GoPGF gene

Expression

Plant

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pVS-GhOMT1_2

Plasmid

#234904

Purpose

This all-in-one vector is used to specifically silence the GhOMT1 gene via virus-induced gene silencing (VIGS).

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

VIGS fragement 2 of GhOMT1 gene

Expression

Plant

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pVS-GhOMT1_1

Plasmid

#234903

Purpose

This all-in-one vector is used to specifically silence the GhOMT1 gene via virus-induced gene silencing (VIGS).

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

VIGS fragement 1 of GhOMT1 gene

Expression

Plant

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCHMP3-NS3-Blue

Plasmid

#223533

Purpose

Mammalian expression of CHMP3 fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a blue fluorescent protein.

Depositor

Winfried Weissenhorn

Article

Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1

Insert

CHMP3 (CHMP3 Human)

Tags

NS3 Hepatitis C protease and mTurquoise

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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