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pLXI_TRC403 SMARCB1 var 2

Plasmid

#111185

Purpose

Inducible vector (V5 tagged) with SMARCB1 variant 2

Depositor

William Hahn

Article

Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

Insert

BAF47 (SMARCB1 Human)

Use

Lentiviral

Tags

V5

Promoter

TRE

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-PDGFRb-HA

Plasmid

#204350

Purpose

Expresses wild-type PDGFRb gene. Used for DNA delivery using Adeno-associated Virus.

Depositor

Hirofumi Nakatomi

Article

Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

Insert

Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)

Use

AAV

Tags

HA

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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NYESO alpha into TRAC HDRT Source (pTR 223)

Plasmid

#112023

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alpha

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO alpha into TRAC HDRT

Use

CRISPR and Synthetic Biology

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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NYESO beta into TRBC1 HDRT Source (pTR 277)

Plasmid

#112024

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR-beta with 1G4 NYESO TCR-beta

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO beta into TRBC1 HDRT

Use

CRISPR and Synthetic Biology

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5-IMPROVED

Plasmid

#154088

Purpose

Improved version to express LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells.

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Ligand Dependent Nuclear Receptor Corepressor, HOXA9, HOXA5 (LCOR, HOXA9, HOXA5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLXI_TRC401 SMARCB1 var 2

Plasmid

#111182

Purpose

Inducible vector (untagged) with SMARCB1 variant 2

Depositor

William Hahn

Article

Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

Insert

BAF47 (SMARCB1 Human)

Use

Lentiviral

Promoter

TRE

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV2-sgTrp53-sgAtrx-EFS-Cre

Plasmid

#189977

Purpose

Expresses Cre cDNA and sgRNAs targeting murine Trp53 and Atrx

Depositor

William Kaelin

Article

Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

Insert

sgTrp53, sgAtrx, Cre recombinase

Use

AAV, Cre/Lox, and Mouse Targeting

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-RUNX1-P2A-ERG-IMPROVED

Plasmid

#154089

Purpose

Improved version to express RUNX1, P2A, ERG in mammalian cells.

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1, ETS-Related Gene (RUNX1, ERG Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pSLIK-NFLAG-hEWSR1G511A-CMyc

Plasmid

#50734

Purpose

Produces lentivirus expressing human EWSR1G511A mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G

Depositor

Zissimos Mourelatos

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 G511A (EWSR1 Human)

Use

Lentiviral

Tags

FLAG and Myc

Mutation

Changed Gly 511 to Ala

Promoter

TRE

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCV-MerCreMer-hygro

Plasmid

#188982

Purpose

Retroviral expression of tamoxifen-inducible Cre recombinase

Depositor

William Kaelin

Article

Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

Insert

Tamoxifen-inducible Cre recombinase

Use

Cre/Lox and Retroviral

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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