161,186 results

1. pBS L30 mRuby3-Rab18

   Plasmid

   #166431

   Purpose

   Rab18 fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.

   Depositor

   Raphael Gaudin

   Insert

   Rab18
   

   Use

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   L30 weak promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAblo

   Plasmid

   #207528

   Purpose

   Conditionally-replicating in Pseudomonas plasmid for adenine base editing based on pS44i8GH-2; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; Pm→ ABE:SpRY, PEM7→non-specific sg

   Depositor

   Pablo Ivan Nikel

   Article

   Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

   Insert

   plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS, Pm→repA, P14b→msfGFP; Pm→ ABE:SpRY, PEM7→non-specific sgRNA
   

   Use

   CRISPR; Pseudomonas vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

   Plasmid

   #179105

   Purpose

   pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXS-IRES-Blast POLA1

   Plasmid

   #160807

   Purpose

   Express POLA1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLA1 (POLA1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Codon Optimized

   Promoter

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLK65

   Plasmid

   #37257

   Depositor

   Ann Hubbard

   Article

   Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

   Insert

   vang-like 2 (VANGL2 Human)
   

   Use

   Adenoviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

6. eGFP-hCOL1A1

   Plasmid

   #140110

   Purpose

   Expresses fluorescently-tagged human Type I procollagen α1 chain with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Insert

   Type I procollagen α1 (COL1A1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   COL1A1 exons 2-3 replaced by fluorescent tag, K20…

   Promoter

   CMV

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMIR-FLAG-MLL-AF9

   Plasmid

   #71444

   Purpose

   Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2

   Depositor

   Daisuke Nakada

   Article

   Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

   Insert

   MLL-AF9 (KMT2A Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFT4.2

   Plasmid

   #208807

   Purpose

   Expresses the fluorescent protein phiLOV2.1

   Depositor

   François-Xavier Campbell-Valois

   Article

   Tchagang et al Front Microbiol. 2023 Oct 20;14:1245755.

   Insert

   phiLOV2.1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti6/V5-DEST-MTHFD2

   Plasmid

   #31213

   Depositor

   Lynda Chin

   Article

   Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

   Insert

   MTHFD2 (MTHFD2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiCRISPR-mCherry

    Plasmid

    #75161

    Purpose

    Derived from LentiCRISPR v1 but expresses mCherry instead of puromycin resistance. 3rd generation.

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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