We narrowed to 5,441 results for: SPI

1. pGBW-m4134904

   Plasmid

   #151930

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 spike prefusion-stabilized

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 spike prefusion-stabilized (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   d1-15 d1209-1273 R682A R685G K986P V987P

   Promoter

   CMV

   Available since

   May 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pGBW-m4258326

   Plasmid

   #154793

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 surface glycoprotein (Spike protein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;V5

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available since

   July 30, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pαH-S-GSAS-2P-OMICRON

   Plasmid

   #180593

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

   Insert

   Spike (S-GSAS-2P-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pαH-S-GSAS-OMICRON

   Plasmid

   #180423

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

   Insert

   Spike (S-GSAS-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEM03

   Plasmid

   #135417

   Purpose

   Expression of Hygromycin resistance from Spizellomyces Hsp70 promoter

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   SpunHsp70pr
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Hsp70pr

   Available since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEM09

   Plasmid

   #135479

   Purpose

   Spizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFP

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   SpunHsp70pr
   

   Use

   Tags

   GFP and hph

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Spun Hsp70pr

   Available since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1_puro_CAG_HexaPro

   Plasmid

   #164077

   Purpose

   Targeted integration of SARS-CoV-2 spike HexaPro variant into the AAVS1 genomic safe harbor locus

   Depositor

   Yannick Doyon

   Article

   Rivest et al protocols.io 2021

   Insert

   SARS-CoV-2 S HexaPro (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   CRISPR

   Tags

   2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …

   Expression

   Mammalian

   Mutation

   Ectodomain only (AAs 1-1208); 682-685 (furin site…

   Promoter

   CAG

   Available since

   Jan. 4, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pFUW-tetO-Pu.1

   Plasmid

   #139839

   Purpose

   doxycycline-inducible expression of mouse Pu.1 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   Pu.1 (Spi1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-mCMV

   Available since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF1656

   Plasmid

   #141861

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SPIB (SPIB Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF1655

    Plasmid

    #142817

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SPIB (SPIB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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