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pTS117 His14-Avi-45xGS-anti GFP nanobody

Plasmid

#199370

Purpose

Bacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-GFP nanobody

Use

Tags

14xHis-Avi

Expression

Bacterial

Mutation

WT

Promoter

T5-LacO

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS118 His14-Avi-28xGS-anti ALFA tag nanobody

Plasmid

#199371

Purpose

Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-ALFA nanobody

Use

Tags

14xHis-Avi

Expression

Bacterial

Mutation

WT

Promoter

T5-LacO

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 BS (-) N protein

Plasmid

#188448

Purpose

SARS CoV-2 N protein expression

Depositor

Ajit Chande

Article

Mishra et al Life Sci Alliance. 2022 Mar 16;5(7). pii

Insert

SARS-CoV-2 N protein

Use

Tags

Strep-Tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

Plasmid

#199390

Purpose

Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-ALFA nanobody

Use

Tags

14xHis-Avi-SUMOEu

Expression

Bacterial

Mutation

Promoter

T5-LacO

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pHub10-His-YFP-PIP2;1

Plasmid

#99386

Purpose

overexpression and isolation of recombinant At-PIP2;1 protein

Depositor

Ning Li

Article

Qing et al Mol Plant. 2016 Jan 4;9(1):158-74. doi:

Insert

PIP2;1 (PIP2A Mustard Weed)

Use

Tags

His and YFP

Expression

Plant

Mutation

Promoter

2xCaMV35S

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pADSL-2XStrep

Plasmid

#125684

Purpose

expresses human ADSL protein with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

ADSL (ADSL Human)

Use

Tags

2X Strep-Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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FMR1-E17B-P2A-NLuc

Plasmid

#157857

Purpose

donor plasmid for FMR1-Nluc

Depositor

Xinyu Zhao

Article

Li et al Stem Cells. 2017 Jan;35(1):158-169. doi:

Insert

FMR1-Nlu (FMR1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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UASp-deltaN cycB

Plasmid

#112967

Purpose

P element vector for transposon insertion of nondegradable Cyclin B into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Lee et al Genetics. 2001 Aug;158(4):1545-56.

Insert

cycB (CycB Fly)

Use

P insertion vector

Tags

Expression

Mutation

N-term deletion of first 46 amino acids, includes…

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-p15 Bid-KKAA-GFP

Plasmid

#191970

Purpose

Expression of the active p15 cBid KKAA mutant, chimera with GFP.

Depositor

Luca Scorrano

Insert

p15 - cBid KKAA

Use

Tags

Expression

Mammalian

Mutation

KKAA: Lys 157 and Lys 158 into Ala

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pUC-MLXγ

Plasmid

#211755

Purpose

Cloning vector for the expression of mouse MLXγ

Depositor

Russell Goodman

Article

Singh et al Cell Metab. 2024 Jan 2;36(1):144-158.e7.

Insert

MLX (Mlx Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ADSL_pLX307

Plasmid

#98311

Purpose

Lentiviral expression of ADSL

Depositor

Sefi Rosenbluh, William Hahn

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

ADSL (ADSL Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pUC-ChREBPα

Plasmid

#211754

Purpose

Cloning vector for the expression of mouse ChREBPα

Depositor

Russell Goodman

Article

Singh et al Cell Metab. 2024 Jan 2;36(1):144-158.e7.

Insert

MLXIPL (Mlxipl Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmBelle_B

Plasmid

#145912

Purpose

Insect Expression of DmBelle

Depositor

Elisa Izaurralde

Insert

DmBelle (bel Fly)

Use

Tags

Expression

Insect

Mutation

del 158-160 (GGG)

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmBelle_1-298_B

Plasmid

#145913

Purpose

Insect Expression of DmBelle_1-298

Depositor

Elisa Izaurralde

Insert

DmBelle_1-298 (bel Fly)

Use

Tags

Expression

Insect

Mutation

del 158-160 (GGG)

Promoter

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-UBC9

Plasmid

#228949

Purpose

express a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag)

Depositor

Marcin Suskiewicz

Insert

UBC9 (UBE2I Human)

Use

Tags

His tag and thrombine cleavage site : MGHHHHHHSSG…

Expression

Bacterial

Mutation

Promoter

T7-lac

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-C2gamma

Plasmid

#21215

Depositor

Tobias Meyer

Article

Codazzi et al Curr Biol. 2001 Jul 24. 11(14):1089-97.

Insert

GFP-pcDNA3-PKCgamma-c2 (Prkcg Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

The C2-domain from PKCgamma (aa170-260) plus flan…

Promoter

Available since

July 13, 2009

Availability

Academic Institutions and Nonprofits only

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pB-actin E6 TTL, E7

Plasmid

#13716

Depositor

Karl Munger

Article

Münger et al J Virol. 1989 Oct . 63(10):4417-21.

Insert

HPV 16 E6 E7 (E6, E7 Human)

Use

Tags

Expression

Mammalian

Mutation

Translation termination linker at bp 158 of E6, s…

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pMSCV_IRES_GFP_CD16a

Plasmid

#196189

Purpose

Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

Depositor

Ana Ruiz-Saenz

Article

Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

Insert

CD16a (FCGR3A Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

changed Phenylalanine 158 to Valine

Promoter

LTR

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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3XFlag pCMV5-TOPO TAZ (∂WW)

Plasmid

#24811

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of amino acids 111-158.

Promoter

Available since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pSLIK_hygro_3xflag_NRF2(RR)

Plasmid

#136535

Purpose

Lentiviral expression vector for an inducible 3xflag-tagged NRF2(a1584c_a1586t_a1589g)

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

NRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)

Use

Lentiviral; Destinatioin vector for gateway cloni…

Tags

3x FLAG

Expression

Mammalian

Mutation

This plasmid is developed by mutating 3 base pair…

Promoter

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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