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Showing: 1221 - 1240 of 4184 results

  3. HA tagged mid ELL2 M133, 138, 186ILeu

    Plasmid
    #127276
    Purpose
    Expresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains middle HA tag that doesn't disrupt function
    Depositor
    Christine Milcarek
    Article
    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi
    Insert
    ELL2 (ELL2 Human)
    Expression
    Mammalian
    Mutation
    M133, 138, 186 to Ileu, HA tag at XbaI after Met …
    Promoter
    pEF1a
    Available Since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127276-plasmid-map-sequence-id-249998

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  4. pET-30a(+)-Syk-tSH2-FX

    Plasmid
    #111272
    Purpose
    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20-amino-acid linker (GGS)3GS(GGS)3, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20aa linker (Syk Mouse)
    Expression
    Bacterial
    Mutation
    interdomain A (Phe 119 to His 162) substituted by…
    Promoter
    T7 promoter
    Available Since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111272-plasmid-map-sequence-id-215700

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  17. TFORF1082

    Plasmid
    #142724
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FUBP1 (FUBP1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    142724-plasmid-map-sequence-id-462889

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  19. pLKO_HSV.Ollas.HA_NGFR

    Plasmid
    #158272
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HSV.Ollas.HA
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158272-plasmid-map-sequence-id-328146

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Showing: 1221 - 1240 of 4184 results