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pZS2katGp-RBS31-PhiC31-proD-oxyR

Plasmid

#78213

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Publication

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pBAC Bxbi TetR

Plasmid

#78226

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Publication

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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Bxbi GFP MCP Reporter

Plasmid

#78225

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Publication

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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Bxbi+TP901 GFP Bandpass BAC Reporter

Plasmid

#78229

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Publication

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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PhiC31 GFP BAC Reporter

Plasmid

#78214

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Publication

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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mP14H1

Plasmid

#84369

Purpose

antigen for mouse PRDM14 purification ( aa 1-231)

Depositor

Danny Reinberg

Publication

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14

Tags

6xHis

Expression

Bacterial

Mutation

only aa 1-231 present

Promoter

T7

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAct:MCP-VP64

Plasmid

#78904

Purpose

Expresses MCP:VP64 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Publication

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

MS2:VP64

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available since

March 25, 2017

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-AGMpygTRIM5-FOS

Plasmid

#79064

Purpose

Express African green monkey pygerythrus TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5alpha(African green monkey pygerythrus)-FOS

Tags

OneSTrEP-FLAG

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(-) + SLNCR1 _462-699

Plasmid

#86819

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

Depositor

Carl Novina

Publication

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Expression

Mammalian

Promoter

CMV

Available since

March 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(-) + SLNCR1 _568-637

Plasmid

#86820

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

Depositor

Carl Novina

Publication

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Expression

Mammalian

Promoter

CMV

Available since

March 1, 2017

Availability

Academic Institutions and Nonprofits only

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AP1297‐1

Plasmid

#87247

Purpose

gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)

Depositor

Geraldine Seydoux

Publication

Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

Insert

gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)

Tags

gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::…

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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hP14H8y

Plasmid

#84368

Purpose

antigen for human PRDM14 purification ( aa 1-243)

Depositor

Danny Reinberg

Publication

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14 (PRDM14 Human)

Tags

6xHis

Expression

Bacterial

Mutation

contains PRDM14 AA1-243

Promoter

T7

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-OSF-TRIM5-21R

Plasmid

#79063

Purpose

Gateway entry vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5-21R

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

T7

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIMCyp (owl monkey)(K283D, Q287D)

Plasmid

#79036

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5Cyp (owl monkey) (K283D, Q287D)

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Changed Lys 283 to Asp and Gln 287 to Asp

Promoter

polyhedrin

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(chimpanzee)

Plasmid

#79032

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(chimpanzee)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

polyhedrin

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAav-MDM2-PQS2-3xHA

Plasmid

#84886

Purpose

rAAV-based template for genome engineering of the MDM2 protein C-terminus containing PQS2 and 3xHA tags and a selection cassette

Depositor

Sven Eyckerman

Publication

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Inserts

MDM2 genomic sequence HR1 (MDM2 Human)
MDM2 genomic insert HR2 (MDM2 Human)

Use

AAV

Tags

PQS2 3xHA

Promoter

no

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Six4

Plasmid

#84323

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Six4. Not for gene expression.

Depositor

Peter Mombaerts

Publication

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Six4 (Six4 Mouse)

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Prrx1

Plasmid

#84322

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Prrx1. Not for gene expression.

Depositor

Peter Mombaerts

Publication

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Prrx1 (Prrx1 Mouse)

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Pou2f3

Plasmid

#84321

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pou2f3. Not for gene expression.

Depositor

Peter Mombaerts

Publication

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Pou2f3 (Pou2f3 Mouse)

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Pbx1

Plasmid

#84317

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pbx1. Not for gene expression.

Depositor

Peter Mombaerts

Publication

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Pbx1 (Pbx1 Mouse)

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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