We narrowed to 2,422 results for: NIG

1. pLV[Exp]-Puro-EF-1α>{H2B-RFP}

   Plasmid

   #201121

   Purpose

   RFP expression in cells under EF-1α promoter; cytoplasmic and histone-bound, nuclear

   Depositor

   Ori Bar-Nur

   Article

   Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

   Insert

   H2B-RFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   No

   Promoter

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-N1-PARP1

   Plasmid

   #211578

   Purpose

   CMV driven PARP1-eGFP expressing construct with IRES-Neomycin selection

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

   Plasmid

   #131004

   Purpose

   bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of human Synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Has Service

   AAV8

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   Kv2.1-HA

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. WP_010880027.1

   Plasmid

   #194729

   Purpose

   Lumazine synthase of the hyperthermophile Aquifex aeolicus bacterium

   Depositor

   Fang Li

   Article

   Geng et al PLoS Pathog. 2021 Sep 7;17(9):e1009897.

   Insert

   nanoparticle-proteinA and His tag
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFB1.HMBP.PrS.MTF2

   Plasmid

   #125169

   Purpose

   Expresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   MTF2 (MTF2 Human)
   

   Use

   Tags

   His-MBP

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV2_CAG_oROS-HT_WPRE

   Plasmid

   #216414

   Purpose

   Encodes the genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   oROS-HT
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-PARP1–eGFP

   Plasmid

   #211552

   Purpose

   A piggyBac vector containing CMV-PARP1-eGFP-IRES-Neo cassette.

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Piggybac

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE

   Plasmid

   #130995

   Purpose

   AAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of human synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Has Service

   AAV8

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   mScarlet-Kv2.1

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-FNLS-P2A-Puro

   Plasmid

   #110841

   Purpose

   Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-FNLS
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D10A and NLS sequence at the N-terminus

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAVS1-A2UCOE-EF1a-iCasp9-P2A-Crry-T2A-mCD59-E2A-Qa1 SCT-F2A-neo-AAVS1

    Plasmid

    #205445

    Purpose

    human AAVS1-targeting plasmid for expression of iCasp9, Crry, mouse CD59, and Qa1 SCT

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    iCasp9-Crry-mCD59-Qa1 SCT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHR-TRE3G-dCas9-10X GCN4-P2A-mCherry

    Plasmid

    #122132

    Purpose

    Express a dCas9, 10X GCN4, and mCherry cassette from the TRE3G promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    dCas9-10X GCN4-P2A-mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    dCas9 D10A & H840A

    Promoter

    TRE3G

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFB1.HMBP.PrS.PHF1

    Plasmid

    #125167

    Purpose

    Expresses human PHF1 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    PHF1 (PHF1 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV2-CAG-mGreenLantern-KASH

    Plasmid

    #191094

    Purpose

    To express a bright monomeric green FP to label the eucaryotic nuclear envelope. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Murray Blackmore, Pantelis Tsoulfas

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    mGreenLantern-KASH
    

    Use

    AAV and Synthetic Biology

    Tags

    mGreenLantern fused to the KASH domain of Nesprin…

    Expression

    Mammalian

    Mutation

    Optimizzation was done using the Human codon usage

    Promoter

    CMV enhancer and CAG

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-TRE3G-FNLS-PGK-Puro

    Plasmid

    #110847

    Purpose

    Lentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA-FNLS
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D10A and NLS sequence at the N-terminus

    Promoter

    TRE3G

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pC1-dmito-oROS-HT

    Plasmid

    #216418

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to mitochondrial matrix.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    dmito-oROS-HT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOTTC385 - pAAV CMV-IE IRES EGFP

    Plasmid

    #102936

    Purpose

    An AAV vector that expresses IRES EGFP under the CMV-IE promoter

    Depositor

    Brandon Harvey

    Article

    Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-PH-TAPP1

    Plasmid

    #161985

    Purpose

    Expresses GFP-tagged PH-Tapp1 in mammalian cells (PI(3,4)P2 biosensor)

    Depositor

    Dario Alessi, David Bryant

    Article

    Posor et al Nature. 2013 Jul 11;499(7457):233-7. doi

    Insert

    PH-TAPP1 (PLEKHA1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAVS1-A2UCOE-EF1a-hCD46-P2A-HLA-G SCT-T2A-hCD47-E2A-hCD59-F2A-neo-AAVS1

    Plasmid

    #205443

    Purpose

    human AAVS1-targeting plasmid for expression of iCasp9, HLA-E SCT, human and CD55

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    hCD46-HLA-G SCT-hCD47-hCD59
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5FRT_KCNQ3

    Plasmid

    #173163

    Purpose

    To generate Flp-In CHO-KCNQ3 stable cells

    Depositor

    Al George

    Article

    Vanoye et al JCI Insight. 2022 Mar 8;7(5):e156314. do

    Insert

    KCNQ3 (KCNQ3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pC1-oROS-HT-CaaX

    Plasmid

    #216417

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the intracellular site of cell membranes.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT-CaaX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge