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pME-AKT-PH

Plasmid

#67674

Purpose

Gateway entry clone containing AKT-PH with no stop codon

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

AKT-PH

Use

Gateway multisite middle entry clone

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_CAG

Plasmid

#62420

Purpose

MXS_chaining vector with CAG

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

CAG Promoter

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_SV40pA

Plasmid

#62426

Purpose

MXS_chaining vector with SV40pA

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

SV40 poly A

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pPOTv7-puro-OsAID (p5109)

Plasmid

#239359

Purpose

pPOTv7 based plasmid template for addition of a Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using puromycin

Depositor

Mark Carrington

Publication

Alexandra et al bioRxiv 2025.06.17.660065

Insert

Rice Auxin Inducible Degradation domain

Use

Bacterial

Promoter

none

Available since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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iTol2Amp-γ-crystallin:venusGFP

Plasmid

#108462

Purpose

This plamid can be used to recombine the iTol2-Amp-γ-crystallin:RFP into a BAC.

Depositor

Nadia Mercader Huber

Publication

Sanchez-Iranzo et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

iTol2Amp crystGFP

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ157

Plasmid

#98413

Purpose

Pombe Expression Vector - nmt81 promoter with Sp.ura4 marker

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Expression

Yeast

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-OSBP

Plasmid

#67677

Purpose

Multisite gateway entry clone for adding C-terminal fusions of OSBP

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

OSBP

Use

Gateway multisite 3' entry clone

Mutation

aa 87-185 of NP_002547.1

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_bGpA

Plasmid

#62424

Purpose

MXS_chaining vector with bGpA

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

beta-Globin poly A

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMX-mTAF6

Plasmid

#44337

Depositor

Marc Timmers

Publication

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF6

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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UXS1

Plasmid

#39162

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

UXS1 (UXS1 Human)

Tags

His Tev

Expression

Bacterial

Mutation

codon-optimized

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pJBM3

Plasmid

#225290

Purpose

E. coli-Campylobacter shuttle vector; KanR

Depositor

Robert Moore

Publication

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Replication elements from pCC388 for pJBM3

Expression

Bacterial

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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NK644

Plasmid

#109096

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Publication

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PTSG_00306 geranyl-geranylation signal

Use

Choanoflagellate expression

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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MXS_P2A

Plasmid

#62433

Purpose

MXS_chaining vector with P2A

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Self-cleaving peptide

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_tTA2

Plasmid

#62429

Purpose

MXS_chaining vector with rTA2

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Tetracycline transactivator

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mCardinal

Plasmid

#179796

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCardinal

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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MXS_H2B

Plasmid

#62432

Purpose

MXS_chaining vector with H2B

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Histone 2B with leading Kozak sequence

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pFUSE-rIgG-Fc2-MFSD8(L9)-Zeo

Plasmid

#239947

Purpose

Expression of MFSD8 loop 9 tagged with rabbit IgG Fc2

Depositor

Jan Carette

Publication

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

Fc2-MFSD8(L9)

Expression

Bacterial and Mammalian

Available since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pPOTv6-blast-His:mNG:His

Plasmid

#201094

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mNeonGreen

Use

Unspecified

Promoter

read through

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pABY-c25

Plasmid

#201769

Purpose

Expresses mNeonGreen-tagged NbALFA under CYC1 promoter in yeast

Depositor

Saravanan Palani

Publication

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

NbALFA

Tags

40aa Linker, mNeonGreen

Expression

Yeast

Promoter

CYC1

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-tdTomato

Plasmid

#179794

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

tdTomato

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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