Addgene

Showing: 1221 - 1240 of 9114 results

Results per page:

Sort results by:

pABY-T1

Plasmid

#201764

Purpose

For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

ALFA tag

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pABY-T3

Plasmid

#201766

Purpose

For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

ALFA tag

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pABY-T4

Plasmid

#201767

Purpose

For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

ALFA tag

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuet1_CylLL_CylM

Plasmid

#208759

Purpose

Expresses modified cytolysin L (mCylLL) in E. coli

Depositor

Wilfred van der Donk

Article

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CylLL
CylLM

Use

Tags

Hisx6 Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuet1_CylLS_CylM

Plasmid

#208760

Purpose

Expresses modified cytolysin S (mCylLS) in E. coli

Depositor

Wilfred van der Donk

Article

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CylLS
CylLM

Use

Tags

Hisx6 Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Src-EGFP

Plasmid

#110496

Purpose

Fluorescent Src

Depositor

Bernardo Mainou, Terence Dermody

Article

Mainou et al J Virol. 2011 Apr;85(7):3203-13. doi: 10

Insert

c-Src (Src Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCVpuro-tdTomato

Plasmid

#97079

Purpose

Retroviral plasmid containing tdTomato for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

tdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
APRT

Plasmid

#64028

Purpose

Expression of S. crervisiae strain APRTase in E. coli. Contains N-terminal HistidineX6 tag.

Depositor

Vern Schramm

Article

Shi et al Biochemistry. 2001 Sep 11;40(36):10800-9

Insert

adenine phosphoribosyltransferase (APT1 Budding Yeast)

Use

Tags

Histidine x 6

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pLEXY (pDN137)

Plasmid

#72656

Purpose

golden gate entry vector for mCherry-LEXY tagging of proteins (no constitutive NLS)

Depositor

Roland Eils, Barbara Di Ventura

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Type

Empty backbone

Use

Golden gate entry vector

Tags

mCherry-LEXY

Expression

Mammalian

Mutation

Promoter

CMV-F

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXCA3H

Plasmid

#47072

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), Avi-tag, and…

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pJBM4

Plasmid

#225291

Purpose

E. coli-Campylobacter shuttle vector; TetR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Tet(O) and its putative promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEZT-BM

Plasmid

#74099

Purpose

Multi-purpose rapid titer bacmam expression vector

Depositor

Ryan Hibbs

Article

Morales-Perez et al Structure. 2016 Mar 24. pii: S0969-2126(

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Bacmam, baculovirus

Tags

EGFP following p10 promoter

Expression

Mammalian

Mutation

Promoter

CMV, p10

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_chaining vector

Plasmid

#62394

Purpose

Empty Backbone

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSO2002

Plasmid

#25842

Depositor

Aziz Sancar

Article

Ozgur et al Biochemistry. 2003 Mar 18. 42(10):2926-3

Insert

human Cryptochrome 2 (CRY2 Human)

Use

Tags

flag, his, and myc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pYEXNH3

Plasmid

#49024

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAN3938

Plasmid

#74697

Purpose

Circuit plasmid: 0xF6

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

Circuit 0xF6

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAN4036

Plasmid

#74698

Purpose

Output plasmid: PPhlF-PAmtR-YFP

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PPhlF-PBetI-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR4-V5 (w71-3)

Plasmid

#17425

Purpose

Gateway entry vector for N-term V5 tag, frame A

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-CMV-dsRed-UBC-GFP-W

Plasmid

#24526

Depositor

Darrell Kotton

Article

Wilson et al Am J Respir Cell Mol Biol. 2008 Aug . 39

Type

Empty backbone

Use

Lentiviral

Tags

DsRed and GFP

Expression

Mutation

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-hsGFP

Plasmid

#64912

Purpose

Encodes a Fluorescent Reporter for Hydrogen Sulfide in Mammalian Cells

Depositor

Huiwang Ai

Article

Chen et al Biochemistry. 2014 Sep 23;53(37):5966-74

Insert

hsGFP

Use

Tags

6xHis

Expression

Mammalian

Mutation

Chromophore tyrosine of the protein mutated to TA…

Promoter

CMV

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge