We narrowed to 45,012 results for: cha
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Plasmid#165128Purposemammalian expression and localizationDepositorInsertNsp13 deltaN2 (ORF1ab codon optimized SARS-COV-2)
TagsEGFPExpressionMammalianPromoterCMVAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nsp14D-EGFP
Plasmid#165125Purposemammalian expression and localizationDepositorAvailable SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
LIC-Z-YFP
Plasmid#153544Purposeperform light induced optogenetic clustering of TCR ζ-Chain tagged with yellow fluorescent protein as described in the associated publicationDepositorInserthuman TCR ζ-Chain (CD247 Human)
TagsVenus YFPExpressionMammalianMutationwith mCherry in LIC-Z replaced by YFPPromoterCMVAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUDR217
Plasmid#113872Purposeexpression of Cas9 programming sgRNA9 and sgRNA10 targetting MPH2-3 and MAL11 respectivelyDepositorInsertsgRNA9-MPH2-3 sgRNA10-MAL11
UseCRISPRExpressionYeastAvailable SinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFrt-invCAG-Luc
Plasmid#63577PurposeShuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional Luciferase expression upon integration. The vector contains an FRT site and two mutant loxP sitesDepositorInsertStop-Lox71-Luciferase-pA-FRT-Lox66-reverseCAG
UseCre/Lox; Frt/flpe recombination mediated insertionExpressionMammalianAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HF1-PGK-Puro
Plasmid#110857PurposeLentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)DepositorInsertCas9-HF1
UseLentiviralTags3X FLAGMutationN497A, R661A, Q695A, Q926APromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FgßF3-ddFLN4-HIS-ybbr
Plasmid#101743Purposemutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forcesDepositorInsertFgßF3 peptide, ddFLN4 fingerprint (FGB Dictyostelium discoideum, Human)
TagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4, added 9F in FgßF3PromoterT7Available SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-VQRRA-PGK-Puro
Plasmid#110858PurposeLentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)DepositorInsertCas9-VQRRA
UseLentiviralTagsFLAGMutationD1135V, R1335Q, T1337R and NLS sequence at the N-…PromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mkgDUX4mqg*
Plasmid#21175DepositorInsertDUX4 (DUX4 Human)
ExpressionBacterial and MammalianMutationPoint mutation at nucleotide 6163 (numbering acco…Available SinceAug. 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mkgDUX4mal*
Plasmid#21172DepositorInsertDUX4 (DUX4 Human)
ExpressionBacterial and MammalianMutationPoint mutation at nucleotide 5114 (numbering acco…Available SinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only