We narrowed to 8,601 results for: Set

1. pADH147

   Plasmid

   #90991

   Purpose

   NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Insert

   gRNA conserved, FRT, DS_HIS1
   

   Use

   CRISPR

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNCS BFP

   Plasmid

   #91757

   Purpose

   Bacterial expression of a fluorescent protein, Blue Fluorescent Protein (BFP)

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

   Insert

   Blue Fluorescent Protein
   

   Tags

   Hexa-Histidine, Xpress epitope for detection with…

   Expression

   Bacterial

   Mutation

   GFP (Y66H)

   Promoter

   T7

   Available Since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1 EGFP-p62 HRD

   Plasmid

   #207550

   Purpose

   Homologous recombination donor to integrate and expression cassette for eGFP-p62 in the AAVS1 locus of human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   TET inducible EGFP-P62
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS1 EGFP-LC3B HRD

   Plasmid

   #207551

   Purpose

   Homologous recombination donor to integrate and expression cassette for eGFP-LC3B in the AAVS1 locus of human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   TET inducible EGFP-LC3B
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1 EGFP-GABARAPL1 HRD

   Plasmid

   #207552

   Purpose

   Homologous recombination donor to integrate and expression cassette for EGFP-GABRAPL1 in the AAVS1 locus of human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   TET inducible EGFP-GABARAPL1
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICH47742::p35S-ePEmax3::tEURb7

   Plasmid

   #227692

   Purpose

   Golden Gate level 1, position 2, ePEmax3 expression cassette.

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Insert

   ePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   Nov. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEvolvR-enCas9-PolI3M-TBD

   Plasmid

   #113077

   Purpose

   Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   John Dueber, David Schaffer

   Article

   Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

   Insert

   enCas9-PolI3M-TBD
   

   Expression

   Bacterial

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV2_HLF-ZE

   Plasmid

   #175034

   Purpose

   HLF targeting construct introducing an ires ZsG P2A tEGFR cassette downstream of the human HLF stop codon

   Depositor

   Guy Sauvageau

   Article

   Lehnertz et al Blood. 2021 Sep 9. pii: S0006-4971(21)01

   Insert

   HLF ires ZsGreen P2A tEGFR
   

   Use

   AAV

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV2_HLF-ZP

   Plasmid

   #175035

   Purpose

   HLF targeting construct introducing an ires ZsG P2A Puro cassette downstream of the human HLF stop codon

   Depositor

   Guy Sauvageau

   Article

   Lehnertz et al Blood. 2021 Sep 9. pii: S0006-4971(21)01

   Insert

   HLF ires ZsGreen P2A Puro
   

   Use

   AAV

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Halo-DNAPKcs HRD

    Plasmid

    #207088

    Purpose

    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous DNA-PKcs locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag with internal PuroR cassette flanked by human DNAPKcs locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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