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Plasmid#174337PurposeTemplate for making Rluc mRNA reporter with no pA.DepositorInsertRenilla luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.22-VEGF-HRE::dLUC
Plasmid#128096PurposeHypoxia response element real-time luciferase reporterDepositorInsertHypoxia response element (x5) from VEGF promoter (VEGFA Human)
UseLuciferaseTagsdrives expression of destabilized luciferase (2CP…ExpressionMammalianMutationPromoterinserted VEGF hypoxia response elements (HRE) (x5)Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4_NFAT-RE-CMVmin-BC0538-luc2
Plasmid#227140PurposeBarcoded assay, Ca2+ sensor; barcode BC05038DepositorInsert6x clustered NFAT element linked to CMV minimal promoter driving barcode 05038 and a luciferase reporter gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10-Claudin-1
Plasmid#46387PurposeClaudin-1 promoter reporter assayDepositorInsertCLDN1 Promoter (CLDN1 Human)
UseLuciferaseTagsExpressionMutationPromoterCLDN1 promoterAvailable sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTS2303-Tier1-CoTC-PPGK-NLuc_TB
Plasmid#169548PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertCoTC-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMVmin-BC0491-luc2
Plasmid#227133PurposeBarcoded assay, cAMP/Ca2+ sensor; barcode BC0491DepositorInsert6x clustered CRE element linked to CMV minimal promoter driving barcode 0491 and a luciferase reporter gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
Syn-ATP
Plasmid#51819PurposeOptical reporter of presynaptic ATPDepositorInsertChimeric Synaptophysin-mCherry-Luciferase
UseTagsExpressionMammalianMutationWithin WT luciferase gene, changed Threonine 214 …PromoterCMVAvailable sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSF4 CMV 5TOP intron renilla TRICK CTE polyA
Plasmid#64544Purpose5′ TOP TRICK reporter mRNADepositorInsert12x PP7 stem-loops
UseTags24xMS2 stem loops, 5' TOP (5′ terminal oligo…ExpressionMammalianMutationPromoterTetracycline-inducible promoter and 5′ UTR of hum…Available sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
IRAK1mut-UTR
Plasmid#15096DepositorInsertIRAK1 3'UTR (IRAK1 Human)
UseLuciferaseTagsExpressionMammalianMutation4-bp substitutions in the miR-146 target sitesPromoterAvailable sinceJune 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
TRAF6mut-UTR
Plasmid#15094DepositorInsertTRAF6 3'UTR (TRAF6 Human)
UseLuciferaseTagsExpressionMammalianMutation4-bp substitutions in the miR-146 target sitesPromoterAvailable sinceJune 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMTS-hFluc-HA-GFP11
Plasmid#177726PurposeFirefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting with an HA tag and the eleventh β-strand of GFP added to the C-terminusDepositorInserthFluc
UseTagsGFP11, HA, and Mitochondrial Targeting SequenceExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPLN-hFluc-DM-eGFP-KDEL
Plasmid#177728PurposeDM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminusDepositorInserthFlucDM
UseTagsKDEL, Prolactin Signal Sequence, and eGFPExpressionBacterial and MammalianMutationR188Q, R261Q (Destabilized Mutant)PromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-1_Rluc-Y39x-Fluc
Plasmid#233615PurposeExpression of Rluc-Fluc reporter gene containing 39xYDepositorInsertRluc-Y39x-Fluc
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pC2.4
Plasmid#226557PurposeNATURA gene encoding plasmidDepositorInsertNATURA reporter gene
UseLuciferase; PiggybacTagsEGFP and FlagExpressionMammalianMutationPromoterEF1alphaAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0410-luc2
Plasmid#227121PurposeBarcoded assay, UAS sensor for split TEV assays; barcode BC0410DepositorInsert10x clustered UAS element linked to CMV minimal promoter driving barcode 0410 and a luciferase reporter gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0432-luc2
Plasmid#227124PurposeBarcoded assay, UAS sensor for split TEV assays; barcode BC0432DepositorInsert10x clustered UAS element linked to CMV minimal promoter driving barcode 0432 and a luciferase reporter gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0434-luc2
Plasmid#227125PurposeBarcoded assay, UAS sensor for split TEV assays; barcode BC0434DepositorInsert10x clustered UAS element linked to CMV minimal promoter driving barcode 0434 and a luciferase reporter gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0407-luc2
Plasmid#227119PurposeBarcoded assay, UAS sensor for split TEV assays; barcode BC0407DepositorInsert10x clustered UAS element linked to CMV minimal promoter driving barcode 0407 and a luciferase reporter gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0436-luc2
Plasmid#227126PurposeBarcoded assay, UAS sensor for split TEV assays; barcode BC0436DepositorInsert10x clustered UAS element linked to CMV minimal promoter driving barcode 0436 and a luciferase reporter gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0400-luc2
Plasmid#227117PurposeBarcoded assay, UAS sensor for split TEV assays; barcode BC0400DepositorInsert10x clustered UAS element linked to CMV minimal promoter driving barcode 0400 and a luciferase reporter gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only