Addgene

We narrowed to 2,920 results for: NAK

Showing: 1241 - 1260 of 2920 results

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pCS2+-dCas9-olEzh2

Plasmid

#180216

Purpose

Ezh2 fused to dCas9 for H3K27me3 epigenome editing

Depositor

Hiroyuki Takeda

Article

Fukushima et al Epigenetics Chromatin. 2019 Mar 13;12(1)

Insert

enhancer of zeste 2 polycomb repressive complex 2 subunit

Use

Tags

FLAG and dCas9

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pFlagCMV2-UbcM8C86A

Plasmid

#12441

Depositor

Dong-Er Zhang

Article

Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

Insert

UbcM8 (Ube2l6 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C86A

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pGL2-promoter-2XMORE

Plasmid

#21112

Depositor

Dean Tantin

Article

Kang et al Genes Dev. 2009 Jan 15. 23(2):208-22.

Insert

2XMORE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2009

Availability

Academic Institutions and Nonprofits only
pGSH0

Plasmid

#45500

Depositor

Nelson Lau

Article

Zeng et al Methods. 2013 May 23. pii: S1046-2023(13

Type

Empty backbone

Use

RNAi

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2013

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1deltaC(E224Q, GiVL-1)

Plasmid

#32033

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-355)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

E224Q and H123-N143 replaced by P642-Y645 of GiDi…

Promoter

T7

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pDESTpK7WG2-RPS5Ap-NLS-OleGFP

Plasmid

#193370

Purpose

nTALEN or nTALECD vector assembly, Destination vector for plants to express ORFs from entry clone with NLS under control of the RPS5A promoter and HSP terminator.

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E2_pENTR_R4-R3_HSPter-RPS5A-NLS

Plasmid

#193371

Purpose

nTALEN or nTALECD vector assembly, Entry clone for plants to express ORFs from entry clone with NLS under control of the RPS5A promoter and HSP terminator.

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1deltaC(E224Q, GiVL-2)

Plasmid

#32035

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-355)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

E224Q and N195-M215 replaced by P697-D714 of GiDi…

Promoter

T7

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pBB535

Plasmid

#27392

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 . 7():32.

Insert

dnaK, dnaJ (dnaJ, dnaK E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2011

Availability

Academic Institutions and Nonprofits only

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CMV-ShadowG

Plasmid

#104620

Purpose

Expresses Shadow G in mammalian cell

Depositor

Hideji Murakoshi

Article

Murakoshi et al Sci Rep. 2015 Oct 15;5:15334. doi: 10.10

Insert

ShadowG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 5, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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APRT

Plasmid

#64028

Purpose

Expression of S. crervisiae strain APRTase in E. coli. Contains N-terminal HistidineX6 tag.

Depositor

Vern Schramm

Article

Shi et al Biochemistry. 2001 Sep 11;40(36):10800-9

Insert

adenine phosphoribosyltransferase (APT1 Budding Yeast)

Use

Tags

Histidine x 6

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pESC-ADE (V5 and HA)

Plasmid

#222489

Purpose

Co-expression of two genes in yeast with V5 and HA tags and ADE2 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Use

Tags

HA and V5

Expression

Yeast

Mutation

Promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pESC-URA (V5 and HA)

Plasmid

#222485

Purpose

Co-expression of two genes in yeast with V5 and HA tags and URA3 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Use

Tags

HA and V5

Expression

Yeast

Mutation

Promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Gamillus/ pcDNA3

Plasmid

#124837

Purpose

mammalian expression of Gamillus, an acid-tolerant monomeric green fluorescent protein

Depositor

Takeharu Nagai

Article

Shinoda et al Cell Chem Biol. 2018 Mar 15;25(3):330-33

Insert

Gamillus

Use

Tags

No tag

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pESC-TRP (V5 and HA)

Plasmid

#222488

Purpose

Co-expression of two genes in yeast with V5 and HA tags and TRP1 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Use

Tags

HA and V5

Expression

Yeast

Mutation

Promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pESC-HIS (V5 and HA)

Plasmid

#222487

Purpose

Co-expression of two genes in yeast with V5 and HA tags and HIS3 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Use

Tags

HA and V5

Expression

Yeast

Mutation

Promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pESC-LEU (V5 and HA)

Plasmid

#222486

Purpose

Co-expression of two genes in yeast with V5 and HA tags and LEU2 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Use

Tags

HA and V5

Expression

Yeast

Mutation

Promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-3(β2)

Plasmid

#160268

Purpose

Expresses a concatemeric construct of a α4:β2 (2:3) nAChR

Depositor

Pierre-Jean Corringer

Article

Prevost et al Cell Mol Life Sci. 2020 May 29. pii: 10.

Insert

3(β2) concatemer

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-3(α4)

Plasmid

#160267

Purpose

Expresses a concatemeric construct of a α4:β2 (3:2) nAChR

Depositor

Pierre-Jean Corringer

Article

Prevost et al Cell Mol Life Sci. 2020 May 29. pii: 10.

Insert

3(α4) concatemer

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pESC-ARG (V5 and HA)

Plasmid

#222493

Purpose

Co-expression of two genes in yeast with V5 and HA tags and ARG1 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Use

Tags

HA and V5

Expression

Yeast

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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