We narrowed to 1,428 results for: aav vector plasmid

1. pSR646

   Plasmid

   #65215

   Purpose

   Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cells

   Depositor

   Robert Kotin

   Article

   Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

   Inserts

   modified AAV-2 rep78
   modified AAV-6 cap
   

   Use

   AAV

   Expression

   Insect

   Promoter

   P10 and polyhedrin

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSR660

   Plasmid

   #65216

   Purpose

   Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells

   Depositor

   Robert Kotin

   Article

   Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

   Inserts

   modified AAV-2 rep78
   modified AAV-8 cap
   

   Use

   AAV

   Expression

   Insect

   Promoter

   P10 and polyhedrin

   Available Since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSR651

   Plasmid

   #65213

   Purpose

   Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells

   Depositor

   Robert Kotin

   Article

   Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

   Inserts

   modified AAV-2 rep78
   modified AAV-1 cap
   

   Use

   AAV

   Expression

   Insect

   Promoter

   p10 and polyhedrin

   Available Since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAnc80L65AAP

   Plasmid

   #92307

   Purpose

   Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAP

   Depositor

   Luk Vandenberghe

   Article

   Landegger et al Nat Biotechnol. 2017 Mar;35(3):280-284.

   Insert

   Ancestral AAV Capsid Anc80L65AAP
   

   Use

   AAV and Synthetic Biology

   Expression

   Mammalian

   Promoter

   Rep2

   Available Since

   Aug. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. hSyn-DIO-somBiPOLES-eGFP

   Plasmid

   #192584

   Purpose

   AAV-vector for bidirectional optogenetic manipulation of Cre-positive neurons

   Depositor

   Simon Wiegert

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Promoter

   human synapsin

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. hSyn-Aion-mKate2

   Plasmid

   #192574

   Purpose

   AAV-vector for optogenetic long-term silencing of neurons

   Depositor

   Simon Wiegert

   Insert

   Aion
   

   Use

   AAV

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

   Promoter

   human synapsin

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

   Plasmid

   #107055

   Purpose

   Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)

   Depositor

   Alex Hewitt

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ef1a-DIO-Aion-citrine

   Plasmid

   #192571

   Purpose

   AAV-vector for optogenetic long-term silencing of Cre-positive neurons

   Depositor

   Simon Wiegert

   Insert

   Aion
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

   Promoter

   Ef1-alpha

   Available Since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. mDlx-Aion-citrine

   Plasmid

   #192572

   Purpose

   AAV-vector for optogenetic long-term silencing of GABAergic neurons

   Depositor

   Simon Wiegert

   Insert

   Aion
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

   Promoter

   minimal Dlx

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. CaMKII-somBiPOLES-ChroME2s-mCerulean

    Plasmid

    #192582

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Promoter

    CaMKIIa(0.4)

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CaMKII-somBiPOLES-ChroME2f-mCerulean

    Plasmid

    #192581

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Promoter

    CaMKIIa(0.4)

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRepCap6

    Plasmid

    #110770

    Purpose

    pRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.

    Depositor

    David Russell

    Article

    Rutledge et al J Virol. 1998 Jan;72(1):309-19.

    Insert

    AAV6 cap, AAV6 rep genes
    

    Use

    AAV

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAC1-V16-Luc

    Plasmid

    #122034

    Purpose

    AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and Luciferase expression

    Depositor

    Liliane Tenenbaum

    Article

    Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

    Insert

    Luciferase
    

    Use

    AAV

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGG-9.47_VP2_AS #2562

    Plasmid

    #74238

    Purpose

    Modified plasmid with a different backbone that yields higher titers of AAV-AS vectors. Encodes VP2-AS chimeric protein with 19 alanines fused to N-terminus of AAV9.47 VP2.

    Depositor

    Miguel Sena-Esteves

    Article

    Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

    Inserts

    AAV2 Rep
    AAV9.47 VP2-AS
    

    Use

    AAV

    Available Since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAR-9.47_VP2_AS

    Plasmid

    #74241

    Purpose

    Original plasmid described in Choudhury et al, 2015 Mol. Ther. for production of AAV-AS vectors. Encodes VP2_AS chimeric protein with 19 alanines fused to the N-terminus of AAV9.47 VP2.

    Depositor

    Miguel Sena-Esteves

    Article

    Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

    Inserts

    AAV2 Rep
    AAV9.47-VP2_AS
    

    Use

    AAV

    Tags

    19 alanines fused to VP2 of AAV9.47

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. hSyn-Aion-ts-mScarlet-ER

    Plasmid

    #192577

    Purpose

    AAV-vector for optogenetic long-term silencing of neurons

    Depositor

    Simon Wiegert

    Insert

    Aion
    

    Use

    AAV

    Tags

    ts-mScarlet-ER

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    human synapsin

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CaMKII-Aion-ts-citrine-ER

    Plasmid

    #192576

    Purpose

    AAV-vector for optogenetic long-term silencing of excitatory neurons

    Depositor

    Simon Wiegert

    Insert

    Aion
    

    Use

    AAV

    Tags

    ts-Citrine-ER

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    CaMKIIa(0.4)

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX601-miniCMV-SaCas9-U6-LacZvsSaCas9 sgRNA

    Plasmid

    #107049

    Purpose

    All-in-one vectors expressing both SaCas9 and LacZ sgRNA

    Depositor

    Alex Hewitt

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX601-miniCMV-SaCas9-U6-YFPvsSaCas9 sgRNA

    Plasmid

    #107050

    Purpose

    All-in-one vectors expressing both SaCas9 and YFP sgRNA

    Depositor

    Alex Hewitt

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAnc80L65

    Plasmid

    #68837

    Purpose

    Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details

    Depositor

    Luk Vandenberghe

    Article

    Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

    Insert

    Ancestral AAV Capsid Anc80L65
    

    Use

    AAV and Synthetic Biology

    Expression

    Mammalian

    Promoter

    Rep2

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge