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pCRRNA-G11-R0

Plasmid

#101805

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Spy 1049

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCRRNA-G0-R11

Plasmid

#101808

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Spy 1049

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRRNA-G0-R18

Plasmid

#101813

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Spy 1049

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRRNA-G14-R0

Plasmid

#101806

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Spy 1049

Available since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRRNA-G10-R0

Plasmid

#101804

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Spy 1049

Available since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-vox-EGFP

Plasmid

#79970

Purpose

mammalian GFP reporter plasmid, target site for Vika recombinase

Depositor

Frank Buchholz

Article

Karimova et al Nucleic Acids Res. 2013 Jan;41(2):e37. d

Insert

2 vox-sites flanking neo in frame with EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCW57-GFP-2A-MCS

Plasmid

#71783

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Article

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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mito-PAGFP

Plasmid

#23348

Purpose

Expresses a mitochondrial matrix-targeted photoactivable GFP that enables detection and quantification of organelle fusion in living cells.

Depositor

Richard Youle

Article

Karbowski et al J Cell Biol. 2004 Feb 16. 164(4):493-9.

Insert

PAGFP

Use

Tags

mito

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2010

Availability

Academic Institutions and Nonprofits only

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Human V5-IRF3-pcDNA3

Plasmid

#32713

Depositor

Saumen Sarkar

Article

Zhu et al J Immunol. 2010 May 15;184(10):5768-76.

Insert

IRF3 (IRF3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2012

Availability

Academic Institutions and Nonprofits only

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pCW57-MCS1-P2A-MCS2 (Hygro)

Plasmid

#80922

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Article

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC CBA-SpCas9.EF1a-BFP.sgLMNA

Plasmid

#98971

Purpose

Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNA and spCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA2

Plasmid

#26678

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 . 11():63.

Insert

Karyopherin Alpha 2 (KPNA2 Human)

Use

Tags

T7 (MASMTGGQQMG)

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA1

Plasmid

#26677

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 . 11():63.

Insert

Karyopherin Alpha 1 (KPNA1 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

KPNA1 sequence contains a reported SNP at S73N

Promoter

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pCAGGS DRR mCherry Donor EF1a BFP

Plasmid

#98896

Purpose

Homologous recombination donor for pDRR. Expresses mCherry after repait by HR.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

Neomycin-T2A-mCherry-GFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFR-mApple

Plasmid

#203773

Purpose

Number and brightness analysis of EGFR oligomerization

Depositor

Thorsten Wohland

Article

Sankaran et al Nat Commun. 2021 Mar 19;12(1):1748. doi:

Insert

EGFR (EGFR Human)

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57-MCS1-P2A-MCS2 (Blast)

Plasmid

#80921

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Article

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0

Plasmid

#174081

Purpose

Expression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.

Depositor

Ryan Mehl

Article

Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

Insert

NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0

Use

Tags

NES-Flag

Expression

Mammalian

Mutation

L270G, Y271L, N311G, C313A

Promoter

CMV

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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p0521s

Plasmid

#62862

Purpose

Episome vector for Phaeodactylum tricornutum (pCC1BAC-based)

Depositor

Hamilton Smith

Article

Karas et al Nat Commun. 2015 Apr 21;6:6925. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Episomal vector for phaeodacty…

Tags

Expression

Mutation

Promoter

Available since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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PB-fLuc=tdT

Plasmid

#120870

Purpose

Piggybac transposon plasmid for live animal optical imaging

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

pcDNA3-fLuc=tdT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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PB-Aka-P2A-GFP

Plasmid

#120867

Purpose

Piggybac transposon plasmid for live animal optical imaging

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

Akaluc

Use

Tags

Expression

Mammalian

Mutation

insert Akaluc and a P2A site

Promoter

CAG Promoter

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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