We narrowed to 5,960 results for: plasmid dna
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Plasmid#163078PurposeExpresses FlipGFP-based activity reporter of SARS-CoV-2 main protease Mpro and also mCherryDepositorInsertFlipGFP-based Mpro activity reporter and mCherry
ExpressionMammalianAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST
Plasmid#127312PurposeExpress 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codonDepositorInsertnanoLuciferase
Tags3xFLAG and PESTExpressionMammalianPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-PlcR(K30/32L)
Plasmid#181831PurposePhospholipid binding-deficient Lyn-PlcR negative-control plasmid.DepositorInsertLyn-PlcR (K30L,K32L)
TagsECFP, EYFP, and N-terminal targeting sequence fro…ExpressionMammalianMutationLysines 30 and 32 in PH domain mutated to leucine.PromoterCMVAvailable SinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-dPlcR(K30/32L)
Plasmid#181833PurposePhospholipid binding-deficient Lyn-dPlcR negative-control plasmid.DepositorInsertLyn-dPlcR (K30L,K32L)
TagsN-terminal targeting sequence from Lyn kinase, dd…ExpressionMammalianMutationLysines 30 and 32 in PH domain mutated to leucine.PromoterCMVAvailable SinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-Intron-CterFlag-MePCE
Plasmid#113549PurposeMammalian expression plasmid that can be used with the Flp-In T-REx system.DepositorAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/GUG-nLuc-3XFLAG-PEST
Plasmid#127314PurposeExpress 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from GUG start codonDepositorInsertnanoLuciferase
Tags3xFLAG and PESTExpressionMammalianPromoterCMVAvailable SinceJune 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-CIBN-mCherry-VAMP2
Plasmid#178573PurposeMammalian expression plasmid of CIBN-VAMP2 of Opto-vTrapDepositorInsertCIBN-mCherry-VAMP2
ExpressionMammalianPromoterCMVAvailable SinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-GRK2RH-Nluc L118A
Plasmid#158606PurposePlasmid expressing GRK2(RH)-Nluc L118A in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain L118A - linker (GGTGGS) - Nluc
ExpressionMammalianPromoterCMVAvailable SinceJune 29, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.2 mir-1-1 reporter hsa-let-7a-1
Plasmid#46670DepositorInserthsa-let-7a-1 (MIRLET7A1 Human)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianPromoterCMVAvailable SinceJuly 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-stop-nLuc-3XFLAG
Plasmid#127308PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codonDepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/CUG-stop-nLuc-3XFLAG
Plasmid#127309PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon, but translation terminated immediately by stop codonDepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/CUG-nLuc-3XFLAG-PEST
Plasmid#127313PurposeExpress 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from CUG start codonDepositorInsertnanoLuciferase
Tags3xFLAG and PESTExpressionMammalianPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/GGG-stop-nLuc-3XFLAG
Plasmid#127311PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from negative control GGG start codon, but translation terminated immediately by stop codonDepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AAA-stop-nLuc-3XFLAG
Plasmid#127310PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from negative control AAA start codon, but translation terminated immediately by stop codonDepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_CoV1_D28
Plasmid#170447PurposeExpress SARS-CoV-1 WT spike protein with a 28aa deletion on the C-terminal tail. Used for SARS1 pseudotyped virus production.DepositorInsertSARS-CoV-1 spike D28 (S SARS-CoV-1)
TagsNAExpressionMammalianMutationlast 28aa deletion in c-terminal tailPromoterCMVAvailable SinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
(302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)
Plasmid#168192PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRsDepositorInsertHA-LaG6-(EAAAK)4-OGT(4)
TagsHA-TagExpressionMammalianPromoterT7/CMVAvailable SinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Tras heavy chain-GSG-SpyTag003
Plasmid#216292PurposeExpresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras FabDepositorInsertTras heavy chain-GSG-SpyTag003
UseAffinity Reagent/ AntibodyTagsHis6 tag, Signal peptide, and SpyTag003ExpressionMammalianPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlpha15-RLuc8
Plasmid#140984PurposeEncodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlpha15-RLuc8 (GNA15 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphai1-RLuc8
Plasmid#140973PurposeEncodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphai1-RLuc8 (GNAI1 Human)
UseSynthetic BiologyExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaQ-RLuc8
Plasmid#140982PurposeEncodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaQ-RLuc8 (GNAQ Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only