We narrowed to 3,167 results for: tef

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pBS-SKII-3XHA-fCLIP-NAT

Plasmid

#188921

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Publication

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-NAT

Expression

Yeast

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAW8-aid-V5-Turg1:kanMX6

Plasmid

#169357

Purpose

C-terminal aid-V5 degron cassette with kanMX6 marker for S. pombe

Depositor

Tony Carr

Insert

auxin-inducible degron (aid)

Tags

1XV5

Expression

Yeast

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1514

Plasmid

#62384

Purpose

pCR2.1 linker-tev-linker-3X-FLAG::KanMX

Depositor

Jonathan Weissman

Publication

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

tev 3x FLAG

Use

Yeast c-terminal tagging vector

Tags

linker-tev-linker-3xflag

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCY 3140-02

Plasmid

#36242

Depositor

Anne Robinson

Publication

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

yEHA

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yEHA-hphMX4

Available since

June 19, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pCRISPEY-GAL-Kan

Plasmid

#232099

Purpose

Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with KanMX selection

Depositor

Jeffrey Lewis

Publication

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FRB-NLS-3HA-HIS3MX6

Plasmid

#228023

Purpose

To integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with HIS3MX6 marker

Depositor

Joseph Reese

Publication

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB9336 (pgRNA_II-1_NatMX)

Plasmid

#161589

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site II-1

Depositor

Irina Borodina

Publication

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAW8-aid-3HA-Turg1:kanMX6

Plasmid

#169355

Purpose

C-terminal aid-3HA degron cassette with kanMX6 marker for S. pombe

Depositor

Tony Carr

Insert

auxin-inducible degron (aid)

Tags

3XHA

Expression

Yeast

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAW8-aid-5Myc-Turg1:kanMX6

Plasmid

#169356

Purpose

C-terminal aid-5Myc degron cassette with kanMX6 marker for S. pombe

Depositor

Tony Carr

Insert

auxin-inducible degron (aid)

Tags

5XMyc

Expression

Yeast

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFA6a-3YFP-kanMX6

Plasmid

#105149

Purpose

Yeast gene targeting

Depositor

David Kovar, Thomas Pollard, Jian-Qiu Wu

Publication

Wu et al J Cell Biol. 2006 Jul 31;174(3):391-402.

Insert

3YFP-kanMX6

Tags

3YFP

Expression

Bacterial and Yeast

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEasyG3_hph

Plasmid

#184919

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.

Depositor

Jeferson Gross

Publication

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-URA-GAL1-fSNAP-3xHA

Plasmid

#188935

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Publication

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-fSNAP-3xHA

Expression

Yeast

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fSNAP-Kan

Plasmid

#188918

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Publication

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fSNAP-Kan

Expression

Yeast

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-mCherry-mNeonGreen-kanMX (pKBJ006)

Plasmid

#173453

Purpose

Contains mCherry-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Publication

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-RFP-hphMX6

Plasmid

#105161

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Publication

Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

Insert

GBP-RFP-hphMX6

Tags

GBP-RFP

Expression

Bacterial and Yeast

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMST-1313

Plasmid

#118076

Purpose

pTEF1-cexA-tCYC1

Depositor

Michael Sauer

Publication

Steiger et al Metab Eng. 2019 Mar;52:224-231. doi: 10.

Insert

pTEF1-cexA-tCYC1

Expression

Yeast

Promoter

pTEF1

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBS1479-HTP-KanMX6

Plasmid

#154085

Purpose

For C-terminal his6-TEV-ProtA-tagging in S. pombe using KanMX6 as resistance marker.

Depositor

Lidia Vasiljeva

Publication

Kilchert et al Cell Rep. 2015 Dec 22;13(11):2504-2515.

Insert

his6-TEV-protein A-KanR

Use

Pcr-based tagging in yeast

Tags

his6-TEV-protein A

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-hphMX6-81nmt1

Plasmid

#105164

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Publication

Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

Insert

hphMX6-81nmt1

Tags

81nmt1

Expression

Bacterial and Yeast

Promoter

81nmt1

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-hphMX6-41nmt1

Plasmid

#105163

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Publication

Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

Insert

hphMX6-41nmt1

Tags

41nmt1

Expression

Bacterial and Yeast

Promoter

41nmt1

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pGS1034

Plasmid

#63796

Purpose

Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal CBP-3xFLAG-TEV-ProtA triple tag and a LEU2 selection marker

Depositor

Monika Golas, Bjoern Sander

Publication

Lin et al Biochim Biophys Acta. 2015 Mar;1854(3):1

Insert

CBP-3xFLAG-TEV-Protein A followed by KlLEU2

Use

E. coli cloning vector

Tags

CBP-3xFLAG-TEV-Protein A

Promoter

none

Available since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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