We narrowed to 1,270 results for: grna cloning vector

1. pTij_sg_mmu_Med6_C_terminal

   Plasmid

   #197869

   Purpose

   CRISPR/Cas9/sgRNA plasmid for cutting Med6 C terminal and building MED6-mEmerald/Halo knock-in mESCs

   Depositor

   James Zhe Liu

   Article

   Dong et al bioRxiv 2022.10.26.513917

   Insert

   Med6 (Med6 Mouse)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTij_sg_mmu_Med1_N_terminal

   Plasmid

   #197870

   Purpose

   CRISPR/Cas9/sgRNA plasmid for cutting Med1 N terminal and building mEmerald/Halo-MED1 knock-in mESCs

   Depositor

   James Zhe Liu

   Article

   Dong et al bioRxiv 2022.10.26.513917

   Insert

   CRSP210 (Med1 Mouse)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAU002 - pTRE3G-CTCF(DELTA1-265)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

   Plasmid

   #156429

   Purpose

   Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   CTCF, rtta3G, TetO3G (Ctcf Mouse)
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Mutation

   DELTA1-265

   Available Since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEN515 - pTRE3G-CTCF(ZFmut)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

   Plasmid

   #156434

   Purpose

   Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA (all Zinc fingers point mutated), targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA.

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   CTCF, rtta3G, TetO3G (Ctcf Mouse)
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Mutation

   All zinc fingers mutated

   Available Since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAU003 - pTRE3G-Cterm_Nterm_switch_CTCF-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

   Plasmid

   #156430

   Purpose

   Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   CTCF, rtta3G, TetO3G (Ctcf Mouse)
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Mutation

   Cterm_Nterm_switched

   Available Since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

   Plasmid

   #156432

   Purpose

   Vector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   CTCF, rtta3G, TetO3G (Ctcf Mouse)
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAd-PR.Cre

   Plasmid

   #192936

   Purpose

   Adenoviral expression vector encoding sgRNAs targeting Trp53 and Rb1 and Cre

   Depositor

   Thorsten Stiewe

   Article

   Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

   Inserts

   Trp53 (Trp53 Mouse)
   Rb1 (Rb1 Mouse)
   

   Use

   Adenoviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 12, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pAd-PR.CC9

   Plasmid

   #192935

   Purpose

   Adenoviral expression vector encoding sgRNAs targeting Trp53 and Rb1, Cre and Cas9

   Depositor

   Thorsten Stiewe

   Article

   Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

   Inserts

   Trp53 (Trp53 Mouse)
   Rb1 (Rb1 Mouse)
   

   Use

   Adenoviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pENTR1A_mmFbxw7(res) (closed)

   Plasmid

   #160105

   Purpose

   Expresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNA

   Depositor

   Rizwan Haq

   Article

   Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

   Insert

   Fbxw7alpha (Fbxw7 Mouse)
   

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAd-PRL.Cre

    Plasmid

    #192937

    Purpose

    Adenoviral expression vector encoding sgRNAs targeting Trp53, Rb1 and Rbl2 and Cre

    Depositor

    Thorsten Stiewe

    Article

    Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

    Inserts

    Rb1 (Rb1 Mouse)
    Trp53 (Trp53 Mouse)
    Rbl2 (Rbl2 Mouse)
    

    Use

    Adenoviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 9, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge