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Showing: 1261 - 1280 of 10991 results

  12. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

    Plasmid
    #171090
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Sth1 dCas9
    Chloramphenicol acetyltransferase
    TagBFP
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…
    Mutation
    D9A, H599A
    Promoter
    TUB1 (Toxoplasma gondii), transcriptionally linke…
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171090-plasmid-map-sequence-id-335786

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  14. pBGE3.0

    Plasmid
    #165988
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Nm/Kan resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    neomycin resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Available Since
    Dec. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    165988-plasmid-map-sequence-id-378724

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  16. pAGE2.0

    Plasmid
    #165984
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Tet resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA2B2C2
    nourseothricin N-acetyl transferase
    tetracycline resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Available Since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    165984-plasmid-map-sequence-id-375867

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  17. pBGE1.0

    Plasmid
    #165986
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Spec resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    spectinomycin/streptomycin resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Available Since
    Oct. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    165986-plasmid-map-sequence-id-375342

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Showing: 1261 - 1280 of 10991 results