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pET27b-ecDHFR-N23PP/S148A

Plasmid

#110829

Purpose

Bacterial expression of E.coli DHFR N23PP/S148A mutant

Depositor

Stephen Benkovic

Article

Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

Insert

ecDHFR

Expression

Bacterial

Mutation

N23 mutated to P23; a proline residue inserted be…

Promoter

T7

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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mEmerald-PCNA-19-NLS-4

Plasmid

#54222

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

Insert

PCNA-19 NLS (PCNA Human)

Tags

SV40NLS and mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pET51b(+)-NADPH-LUCID-2 (C50 29 nM)

Plasmid

#117909

Purpose

Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nM

Depositor

Kai Johnsson

Article

Yu et al Science. 2018 Sep 14;361(6407):1122-1126

Insert

SNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

R98A on eDHFR

Promoter

T7

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX SHF-SH2

Plasmid

#46491

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHF (SHF Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 317-423

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX SHE-SH2

Plasmid

#46490

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHE

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 382-492

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX SHD-SH2

Plasmid

#46489

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHD (SHD Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 227-340

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Rin3-SH2

Plasmid

#46476

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Rin3 (RIN3 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 55-157

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-Paxillin-22

Plasmid

#55092

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Paxillin

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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mTFP1-Rab4a-7

Plasmid

#55507

Purpose

Localization: Ras GTPase, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Rab4a (RAB4A Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-Rab5a-7

Plasmid

#55094

Purpose

Localization: Ras GTPase, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Rab5a

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only