We narrowed to 2,684 results for: Kl

1. Phat2-hs-AKT1-PH-EGFP-6H

   Plasmid

   #187157

   Purpose

   Protein Expression

   Depositor

   Philipp Niethammer

   Article

   Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

   Insert

   ENSG00000142208 (AKT1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. RNAPII_CTD27-52_K5S_S1966C

   Plasmid

   #98683

   Purpose

   Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5S and S1966C

   Depositor

   Nicolas Fawzi

   Article

   Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

   Insert

   RNA polymerase II C-terminal domain (POLR2A Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K1838S, K1859S, K1866S, K1873S, K1887S, S1966C

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. NDUFAB1 C3.3 gRNA

   Plasmid

   #90791

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NDUFAB1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   NDUFAB1 (Guide Designation C3.3) (NDUFAB1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. NDUFAB1 C4.3 gRNA

   Plasmid

   #90793

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NDUFAB1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   NDUFAB1 (Guide Designation C4.3) (NDUFAB1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUAST YFP Rab8 DN

   Plasmid

   #58451

   Purpose

   expression of drosophila YFP Rab8 dominant negative mutant

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab8 (Rab8 Fly)
   

   Use

   Tags

   YFP

   Expression

   Insect

   Mutation

   T22N (dominant negative)

   Promoter

   GAL4

   Available since

   July 21, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pUAST YFP Rab8 WT

   Plasmid

   #58450

   Purpose

   expression of drosophila YFP Rab8

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab8 (Rab8 Fly)
   

   Use

   Tags

   YFP

   Expression

   Insect

   Mutation

   Promoter

   GAL4

   Available since

   July 21, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pUAST td tomato Rab8 WT

   Plasmid

   #58449

   Purpose

   expression of drosophila td tomato Rab8

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab8 (Rab8 Fly)
   

   Use

   Tags

   td Tomato

   Expression

   Insect

   Mutation

   Promoter

   GAL4

   Available since

   July 21, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pREG(R1-R4) #BGV059

   Plasmid

   #48991

   Purpose

   Retroviral Destination vector containing AttR1-AttR4 site. Use with 4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4)

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pCRII-Topo Lpl in situ probe

   Plasmid

   #45630

   Depositor

   Jeffrey Macklis

   Article

   Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

   Insert

   Lpl in situ probe (Lpl Mouse)
   

   Use

   In situ

   Tags

   Expression

   Mutation

   fragment contains bp# 3249-3900 of NM_008509.2

   Promoter

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

10. hs-FL-Kek1-myc2

    Plasmid

    #41170

    Depositor

    Norbert Perrimon

    Article

    Ghiglione et al Cell. 1999 Mar 19;96(6):847-56.

    Insert

    Kek1 (kek1 Fly)
    

    Use

    Tags

    2X myc

    Expression

    Insect

    Mutation

    Promoter

    Hsp70

    Available since

    Jan. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pCMV-Flag-YAP-5SA/S94A

    Plasmid

    #33103

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

    Insert

    YAP (YAP1 )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S61A, S94A, S109A, S127A, S164A, S381A

    Promoter

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDL025_HA-rb_IgG_HC_15F11

    Plasmid

    #193622

    Purpose

    HA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.

    Depositor

    Jennifer DeLuca

    Article

    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

    Insert

    Anti_HA_15F11 rabbit heavy chain
    

    Use

    Tags

    signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pDL026_HA-rb_IgG_LC_15F11

    Plasmid

    #193623

    Purpose

    HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.

    Depositor

    Jennifer DeLuca

    Article

    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

    Insert

    Anti-HA_15F11 rabbit light chain
    

    Use

    Tags

    signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. mTagRFP-T-Golgi-7

    Plasmid

    #58018

    Purpose

    Localization: Golgi Complex, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Golgi (B4GALT1 Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pEGFP-h53BP1 (siRNA resistant)

    Plasmid

    #110301

    Purpose

    Mammalian expression of a EGFP-tagged full length human 53BP1 (resistant to siRNA targeting AGAACGAGGAGACGGTAATAGTGGG)

    Depositor

    Chris Kok-Lung Chan

    Article

    Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

    Insert

    p53-binding protein 1 (TP53BP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    GAACGAGGA to GAGCGGGGC

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-SpCas9-GFP-U6-gRNA

    Plasmid

    #79144

    Purpose

    All-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSC

    Depositor

    Jizhong Zou

    Article

    Stoehr et al JCI Insight. 2019 May 21;5. pii: 128496.

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMX-Gal4-TEAD4

    Plasmid

    #33105

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

    Insert

    TEAD4 (TEAD4 Human)
    

    Use

    Tags

    Gal4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX458-Ef1a-Cas9-H2B-GFP

    Plasmid

    #159654

    Purpose

    Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX458-Ef1a-Cas9-H2B-mCherry

    Plasmid

    #159655

    Purpose

    Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEJS476-pHAGE-TO-Nme dCas9 3XGFP-SgRNA/Telomere-All-in-one

    Plasmid

    #85716

    Purpose

    Expresses dNmeCas9-3XsfGFP and telomere-targeting Nme sgRNA

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Inserts

    Nme dCas9
    telomere sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO and U6

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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