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pcDNA3-H2B-μDpTT

Plasmid

#182328

Purpose

Histone H2B-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

H2B-μDronpa-TagRFP-T (H2BC3 Human)

Use

Tags

Full-length human histone H2B

Expression

Mammalian

Mutation

Dronpa contains H193T mutation.

Promoter

CMV

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 GRK2 S670D

Plasmid

#45756

Depositor

Robert Lefkowitz

Article

Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

Insert

GRK2 S670D (GRK2 Bovine)

Use

Tags

Expression

Mammalian

Mutation

S670D

Promoter

CMV

Available since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3_V5-APEX2-Sec61B

Plasmid

#83412

Purpose

Expressed N-term tagged APEX2 on Sec61B in mammalian cell

Depositor

Hyun-Woo Rhee

Article

Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

Insert

V5-APEX2-Sec61B (SEC61B Synthetic, Human)

Use

Tags

APEX2 and V5

Expression

Mammalian

Mutation

Promoter

CMV-F

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mDIS3-FLAG

Plasmid

#60046

Purpose

Mammalian expression of FLAG-tagged mouse DIS3

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

Insert

DIS3 (Dis3 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 12, 2014

Availability

Academic Institutions and Nonprofits only
alpha-0 CFP in pcDNA3.1

Plasmid

#36396

Depositor

Narasimhan Gautam

Article

Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

Insert

G protein alpha 0 (Gnao1 Rat)

Use

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted into the KpnI-SpeI sites engineered …

Promoter

cmv

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MKK4beta

Plasmid

#15517

Depositor

Roger Davis

Article

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

pCDNA3 Flag MKK4beta (MAP2K4 Human)

Use

Tags

Flag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Flag-Sintbad

Plasmid

#234668

Purpose

expresses SINTBAD (TBKBP1) in mammalian cells

Depositor

Lienhard Schmitz

Article

Saul et al iScience. 2019 Sep 27;19:527-544. doi: 1

Insert

SINTBAD (TBKBP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-a4-delta tail

Plasmid

#80018

Purpose

Mammalian expression of human integrin alpha4 with no tail

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

integrin alpha4 (ITGA4 Human)

Use

Tags

Expression

Mammalian

Mutation

no cytoplasmic tail (amino acids 1 to 1007)

Promoter

CMV

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-MCS-13 x Linker-BioID2-HA-GPI-PSS

Plasmid

#194750

Purpose

To fuse your protein of interest to the N-terminus of GPI-anchored-BioID2

Depositor

Chaoyang Li

Article

Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

Type

Empty backbone

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Glu

Plasmid

#218461

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Glu substitution)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Glu substitution (S )

Use

Tags

Expression

Mammalian

Mutation

Thr1273AGlu substitution

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Asp

Plasmid

#218462

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Asp substitution)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Asp substitution (S )

Use

Tags

Expression

Mammalian

Mutation

Thr1273Asp substitution

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3_attR1-ORF-attR2-V2R-NTEV-TCS-GV-2xHA_DEST

Plasmid

#194386

Purpose

Gateway Destination vector for GPCR split TEV assays with V2R tail

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

attR1-ORF-attR2-V2R-NTEV-TCS-GV-2xHA

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NL-BCL2L1

Plasmid

#113458

Purpose

NanoLuc-BCL2L1 expression vector (positive control together with pcDNA3.1 PA-mCit-BAD)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

BCL2 like 1 (BCL2L1 Human)

Use

Luciferase

Tags

cmyc-NanoLuc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-BACH1

Plasmid

#232269

Purpose

Expression of N-terminal tagged HA-BACH1 (H. sapiens) in human cell lines.

Depositor

Michele Pagano

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

BACH1 (BACH1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B-E993A

Plasmid

#203698

Purpose

expresses ATP10B with the pathogenic mutation E993A

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Use

Tags

Expression

Mammalian

Mutation

E993A

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B-E210A

Plasmid

#203697

Purpose

expresses ATP10B with the catalytic mutation E210A

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Use

Tags

Expression

Mammalian

Mutation

E210A

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B-D433N

Plasmid

#203696

Purpose

expresses ATP10B with the catalytic mutation D433N

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Use

Tags

Expression

Mammalian

Mutation

D433N

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B-V748L

Plasmid

#203702

Purpose

expresses ATP10B with the pathogenic mutation V748L

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Use

Tags

Expression

Mammalian

Mutation

V748L

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B-I1038T

Plasmid

#203700

Purpose

expresses ATP10B with the pathogenic mutation I1038T

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Use

Tags

Expression

Mammalian

Mutation

I1038T

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B-R153X

Plasmid

#203701

Purpose

expresses ATP10B with the pathogenic mutation R153X

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Use

Tags

Expression

Mammalian

Mutation

R153X

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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