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Plasmid#153029PurposeAnion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).DepositorInsertvPyACR_2164382
UseTagseYFPExpressionMammalianMutationvPyACR_2164382 was C-terminally truncated to incl…PromoterCMV (+ enhancer)Available sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
vPyACR_21821
Plasmid#153030PurposeAnion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).DepositorInsertvPyACR_21821
UseTagseYFPExpressionMammalianMutationvPyACR_21821 was C-terminally truncated to includ…PromoterCMV (+ enhancer)Available sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
564aa ELL2 bluescript
Plasmid#127267PurposeFor in vitro translation of human ELL2 lacking COOH regionDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationAddgene Plasmid 127256--SphI site filled in, then…PromoterT7Available sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-tetO-Esrrb
Plasmid#71152PurposeLentiviral plasmid for tetracycline/doxycycline dependent expression of murine EsrrbDepositorInsertEstrogen related receptor, beta (Esrrb Mouse)
UseLentiviralTagsExpressionMammalianMutationPromotertetOAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-RNAPII-A2-14-HA
Plasmid#160689PurposeExpression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains an S-to-A mutation in position 2 of 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.DepositorInsertRNA polymerase II CTD
UseTagsExpressionBacterialMutationContains an S-to-A mutation in position 2 of 14 o…PromoterAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-NFLAG-hEWSR1WT-CMyc
Plasmid#50733PurposeProduces lentivirus expressing human EWSR1 wild type with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2GDepositorInsertEWSR1 (EWSR1 Human)
UseLentiviralTagsFLAG and MycExpressionMutationPromoterTREAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
2xFKBP-mCh-KDEL
Plasmid#173010PurposeFKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.DepositorInsert2xFKBP-mCh-KDEL
UseTagsmCherryExpressionMammalianMutationPromoterChicken beta actin (CAG)Available sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNAintron-CHIKV-nsp3-c3xFLAG
Plasmid#215696PurposeProduces the Chikungunya virus nonstructural protein 3 with a c-terminal 3xFLAG tagDepositorInsertCHIKV nsp3
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1(+)mGAT1-0-GFP
Plasmid#41662DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsGFPExpressionMammalianMutationC-terminal hydrophobic isoleucine residue added a…PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNAintron-CHIKV-nsp1-c3xFLAG
Plasmid#215694PurposeProduces the Chikungunya virus nonstructural protein 1 with a c-terminal 3xFLAG tagDepositorInsertCHIKV nsp1
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNAintron-CHIKV-nsp2-c3xFLAG
Plasmid#215695PurposeProduces the Chikungunya virus nonstructural protein 2 with a c-terminal 3xFLAG tagDepositorInsertCHIKV nsp2
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNAintron-CHIKV-nsp4-c3xFLAG
Plasmid#215697PurposeProduces the Chikungunya virus nonstructural protein 4 with a c-terminal 3xFLAG tagDepositorInsertCHIKV nsp4
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFSynW Substance P-pHluorin IRES mRuby3
Plasmid#195699PurposeLentiviral plasmid encoding first 68 residues of mouse preprotachykinin with a C-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoterDepositorInsertTac1 (Tac1 Mouse)
UseLentiviralTagspHluorinExpressionMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-N MCV ER
Plasmid#37860DepositorUseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterPKGAvailable sinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
GFP-CA-mDia1
Plasmid#45583DepositorInsertConstitutively active mDia1 (CA-mDia1) (Diaph1 Mouse)
UseTagsGFPExpressionMammalianMutationInsert mDia1 contains mutations V161D and N165D i…PromoterCMVAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CB7.CI.CNDP2-flag.WPRE.rBG
Plasmid#132685PurposeAAV plasmid encoding mouse CNDP2 with C-terminal flag tagDepositorInsertCarnosine dipeptidase 2 (Cndp2 Mouse)
UseAAVTagsFlagExpressionMutationPromoterChicken b-actinAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
2xFKBP-smFP(myc)-KDEL
Plasmid#173021PurposeFKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF.DepositorInsert2xFKBP-smFP(myc)-KDEL
UseTagsFKBP and smFP(myc) non-fluorescentExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry.3xFLAG.NOS1APΔC20-WPRE
Plasmid#127865PurposepAAV plasmid expressing an mCherry.3xFLAG.NOS1APΔC20 fusion protein under the hSyn promoterDepositorInsertNos1ap (Nos1ap Mouse)
UseAAVTags3xFLAG and mCherryExpressionMutationdeleted the c-terminal 20 amino acidsPromoterhSynAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry.3xFLAG.NOS1APC20-WPRE
Plasmid#127866PurposepAAV plasmid expressing an mCherry.3xFLAG.NOS1APC20 fusion protein under the hSyn promoterDepositorInsertNos1ap (Nos1ap Mouse)
UseAAVTags3xFLAG and mCherryExpressionMutationC-terminal 20 amino acids onlyPromoterhSynAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1YFP*
Plasmid#41665DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsYFPExpressionMammalianMutation“Monomerizing” YFP A206K mutation; C-terminal hyd…PromoterCMVAvailable sinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1CFP*
Plasmid#41666DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsCFPExpressionMammalianMutation“Monomerizing” CFP A206K mutation; C-terminal hyd…PromoterCMVAvailable sinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-hSOX10-C373T
Plasmid#24734DepositorInsert(sex determining region Y)-box 10 (SOX10 Human)
UseGateway donor vectorTagsExpressionMutationC replaced by T at position 373 resulting in Q125…PromoterAvailable sinceJune 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-hSOX10-C1271T
Plasmid#24736DepositorInsert(sex determining region Y)-box 10 (SOX10 Human)
UseGateway donor vectorTagsExpressionMutationC replaced with T at position 1271 resulting in A…PromoterAvailable sinceMay 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-4
Plasmid#228986PurposeFor bacterial expression of anti-GST nanobody GST-4, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-4
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-2
Plasmid#228984PurposeFor bacterial expression of anti-GST nanobody GST-2, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-2
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-7
Plasmid#228989PurposeFor bacterial expression of anti-GST nanobody GST-7, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-7
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-38
Plasmid#228979PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-38, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertLaTdT-38
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-1
Plasmid#228983PurposeFor bacterial expression of anti-GST nanobody GST-1, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-1
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-3
Plasmid#228985PurposeFor bacterial expression of anti-GST nanobody GST-3, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-3
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-44
Plasmid#228981PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-44, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertLaTdT-44
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only