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pcDNA3.2 mir-1-1 reporter mir16-1.2

Plasmid

#46681

Purpose

Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir16-1.2 (MIR16-1 Synthetic, Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5R DD-FAM98B[1-330]

Plasmid

#211358

Purpose

Transient expression of DD-V5-FAM98B[1-330]

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

DD-FAM98B[1-330] (FAM98B Human)

Tags

DD-V5

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-NIP45 wt

Plasmid

#210266

Purpose

Inducible expression of N-terminally mCherry-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 wt siRES (NFATC2IP Synthetic)

Tags

mCherry

Expression

Mammalian

Mutation

siRES

Promoter

CMV/TetO2

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 wt

Plasmid

#210262

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 wt siRES (NFATC2IP Synthetic)

Tags

EGFP

Expression

Mammalian

Mutation

siRES

Promoter

CMV/TetO2

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCENPBR pc861

Plasmid

#167569

Purpose

Human CENP-B (aa 1-169 containing DNA-binding domain) cloned into pDsRed1-N1 vector (Clontech).

Depositor

Cristina Cardoso

Article

Chagin et al Nucleus. 2019 Dec;10(1):231-253. doi: 10

Insert

Homo sapiens centromere protein B (CENPB Human)

Tags

Ds-Red

Expression

Mammalian

Promoter

CMV

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMeCP2miRFP670 pc3942

Plasmid

#167568

Purpose

The miRFP670-gene is ligated c-terminally to the rat meCP2-gene.

Depositor

Cristina Cardoso

Article

Chagin et al Nucleus. 2019 Dec;10(1):231-253. doi: 10

Insert

MeCp2 (Mecp2 Rat)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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GA46

Plasmid

#196614

Purpose

Plasmid used for the C-terminal tagging of Atp3 with mCherry and the auxin-inducible degron in yeast

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

ATP3::mCherry-AID(71-114)-NatMX (ATP3 Budding Yeast)

Tags

AID(71-114) and mCherry

Expression

Yeast

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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C513-E08: attB5r-<p10p-polyhedrinp>H6-MBP-nostop-attB1r

Plasmid

#162950

Purpose

Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors; allows His6-MBP fusion for the polyhedrin transcribed protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin) with His6-maltose binding protein fusion tag

Use

Synthetic Biology

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pQE30-VRN1

Plasmid

#159531

Purpose

Expresses Arabidopsis thaliana VERNALIZATION1 in E. coli

Depositor

Joshua Mylne

Article

King et al J Biol Chem. 2013 Feb 1;288(5):3198-207.

Insert

VERNALIZATION1 (VRN1 Mustard Weed)

Tags

Six-Histidine tag

Expression

Bacterial

Promoter

T5

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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Met133I, M1381I, M186I ELL2

Plasmid

#127263

Purpose

For in vitro translation of human ELL2 with Met133I, M1381I, M186I

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)

Use

In vitro translation

Mutation

Three Mets (133, 138, 186) to Ileu

Promoter

T7

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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