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iMb2-Control-Mosaic (SO273)

Plasmid

#99750

Purpose

Second generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different membrane localized fluorescent proteins

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2YFP-2A, Mb2Tomato-2A, Mb2-HA-Tfp1

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-LC3B Q116P G120

Plasmid

#129289

Purpose

Expresses 3xFLAG-tagged deconjugation-resistant LC3B in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Changed Glutamine 116 to Proline. Deleted amino a…

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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NLuc-GID4

Plasmid

#200878

Purpose

Mammalian expression of human GID4 with N-terminal NanoLuciferase fusion

Depositor

Stefan Knapp

Article

Owens et al bioRxiv 2023.01.17.524225

Insert

NanoLuciferase-GID4 (C17orf39 Human)

Expression

Mammalian

Mutation

WT

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBIG1b_10xHisTEVATG5_ATG12

Plasmid

#190861

Purpose

Plasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Inserts

ATG5 (ATG5 Human)
ATG12 (ATG12 Human)

Tags

6xHis-TEV

Expression

Bacterial and Insect

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pRosa26-Acceptor (AG103)

Plasmid

#99632

Purpose

To insert the Tre-Tight or UAS ifgMosaic Donor vectors

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Gt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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Pvalb-2A-Flpo Flp-in replacement vector

Plasmid

#61572

Purpose

Recombinase-mediated cassette exchange in ES cells to insert the Flpo recombinase gene at the mouse Pvalb gene stop codon

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Pvalb-2A-Flpo

Use

Recombinase-mediated cassette exchange using flp …

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX4T2-Ste7-EE

Plasmid

#52681

Purpose

expression of constitutively active Ste7 kinase in bacteria

Depositor

Benjamin Turk

Article

Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

Insert

Ste7 (STE7 Budding Yeast)

Tags

GST and Myc

Expression

Bacterial

Mutation

S359E/T363E constitutively active

Promoter

tac

Available Since

April 30, 2014

Availability

Academic Institutions and Nonprofits only
pLX304-BCL-XL-Y195F

Plasmid

#203573

Purpose

Expresses V5-tagged BCL-XL with partial drug resistance to A-1331852 in mammalian cells.

Depositor

Abhishek Chakraborty

Article

Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Tags

V5-tagged

Expression

Mammalian

Mutation

Changed Tyrosine 195 to Phenylalanine for partial…

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mG1_CH3-3

Plasmid

#105853

Purpose

Expression plasmid coding for hybrid heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG3-derived CH3.

Depositor

Joanna Bereta

Article

Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

Insert

IgG1 heavy chain, IgG3 heavy chain (Ighg1, Ighg3 Mouse)

Expression

Mammalian

Mutation

hybrid heavy chain of mouse IgG1 with CH3 derived…

Promoter

hEF1-HTLV prom

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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iMb-Control-Mosaic (IR98.10)

Plasmid

#99748

Purpose

Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different fluorescent and marker proteins localized to the membrane

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

MbYFP, MbTomato, MbKate2

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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