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pZB573_pB1H1_T7-RNAP_LARP-I5CHO_1

Plasmid

#228508

Purpose

Bacterial Hybrid Driver Plasmid

Depositor

Timothy Whitehead

Article

Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

Insert

T7 RNAP, LARP-I5CHO (#1)

Use

Synthetic Biology

Tags

Umu Degron, GTG-start codon

Mutation

R378K; S430P, N433T, S633P, F849I, F880Y; W727G, …

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pZB578_pB1H1_T7-RNAP_LARP-I

Plasmid

#228507

Purpose

Bacterial Hybrid Driver Plasmid

Depositor

Timothy Whitehead

Article

Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

Insert

T7 RNAP, LARP-I

Use

Synthetic Biology

Tags

Umu Degron, GTG-start codon

Mutation

R378K; S430P, N433T, S633P, F849I, F880Y; W727G, …

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-MCS-3AID-HA-2A-mTurquoiseBSD

Plasmid

#225704

Purpose

Empty backbone for lentiviral expression of AID degron tagged POI and mTurquoise fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Tags

T2A-mTurquoise

Expression

Mammalian

Promoter

pEF1A

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-rTetR(SE)-VP64-2A-mCherryBSD

Plasmid

#225695

Purpose

Lentiviral expression of rTetR(SE) fused to VP64 and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

rTetR-VP64

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG and T2A-mCherry

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-rTetR(SE)-KRAB-2A-mTurquoise

Plasmid

#225690

Purpose

Lentiviral expression of rTetR(SE) fused to ZNF10 KRAB and mTurquoise-NLS

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

rTetR-ZNF10 KRAB

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG and T2A-mTurquoise

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNMTMA_MBP_Z_Cav1_H6_Periplasmic_mScarlet

Plasmid

#223703

Purpose

To produce caveospheres with periplasmically encapsulated mScarlet

Depositor

Rob Parton

Article

Luong et al ACS Nano. 2025 Dec 23;19(50):42079-42096

Insert

MBP_Z_Cav1_H6_Periplasmic_mScarlet

Expression

Bacterial

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINE-GFP

Plasmid

#223706

Purpose

For production of EGFP by E.coli

Depositor

Rob Parton

Article

Luong et al ACS Nano. 2025 Dec 23;19(50):42079-42096

Insert

EGFP

Expression

Bacterial

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-ScChd1[Y137A]

Plasmid

#221198

Purpose

ScChd1-aa118-1274 chromatin remodeler with Y137A mutation

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa118-1274)

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

Y137A

Promoter

T7

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Plasmid

#222990

Purpose

Bacterial expression of iGeoCas9(C1) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Use

CRISPR

Tags

CL7, His, and MBP

Expression

Bacterial

Promoter

tac

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.B-GS3

Plasmid

#204760

Purpose

Expression of Cas9 and human H2A.B

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AB1 (H2AFB1 Human)

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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