We narrowed to 2,550 results for: CCS

1. CA173 CMVTO-mono-hIL10-R5A11M-rens-3GS-IL10Ra-c-myc

   Plasmid

   #231214

   Purpose

   CageIL-10-myc

   Depositor

   Xiaojing Gao

   Article

   Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

   Insert

   mono-hIL10-R5A11M-rens-3GS-IL10Ra-c-myc (IL10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N36I, N110I, K117N, F129L

   Promoter

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. CA-0230 EF1a_Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry_Gen2

   Plasmid

   #231207

   Purpose

   Single transcript cageIL-10 (mRens) + ER-renin (H2M) (Lenti)

   Depositor

   Xiaojing Gao

   Article

   Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

   Insert

   Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   L147I, R148H, I203V, L290V

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWT-IFl_WT-IFr

   Plasmid

   #192207

   Purpose

   Wildtype I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Wildtype I-F Leader and wildtype I-F Locus DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pScr-IFl_WT-IFr

   Plasmid

   #192208

   Purpose

   Scrambled I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Scrambled I-F Leader and wildtype I-F Locus DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCon-IFl_Con-IFr

   Plasmid

   #192209

   Purpose

   Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Consensus I-F Leader and Consensus I-F Locus DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCon-IFl_Con-IEr

   Plasmid

   #192210

   Purpose

   Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Consensus I-F Leader and Consensus I-E Locus DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCon-IFl_Con-ICr

   Plasmid

   #192211

   Purpose

   Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Consensus I-F Leader and Consensus I-C Locus DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCon-IFl_Con-IIAr

   Plasmid

   #192212

   Purpose

   Consensus I-F CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Consensus I-F Leader and Consensus II-A Locus DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCon-IEl_Con-IEr

   Plasmid

   #192213

   Purpose

   Consensus I-E CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Consensus I-E Leader and Consensus I-E Locus DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCon-ICl_Con-ICr

    Plasmid

    #192214

    Purpose

    Consensus I-C CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    Consensus I-C Leader and Consensus I-C Locus DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCon-IIAl_Con-IIAr

    Plasmid

    #192215

    Purpose

    Consensus II-A CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    Consensus II-A Leader and Consensus II-A Locus DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX1-IFl_Con-IFr

    Plasmid

    #192216

    Purpose

    Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    I-F leader and I-F repeat construct not studied further in this paper (X1)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX2-IFl_Con-IFr

    Plasmid

    #192217

    Purpose

    Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    I-F leader and I-F repeat construct not studied further in this paper (X2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCKC017

    Plasmid

    #226622

    Purpose

    pLenti wt hTdT template for libraries 1-2

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Insert

    Terminal deoxynucleotidyl transferase (DNTT Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    synonymous mutation in D2, synonymous mutation in…

    Promoter

    CMV

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCKC775

    Plasmid

    #226626

    Purpose

    piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    7xTCF-LEF and EF1a

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCKC764

    Plasmid

    #226625

    Purpose

    piggyBAC pCAG-hCas9-T2A-Unbiased3

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Human, S. pyogenes, Chicken)
    EF1a promoter, blasticidin resistance
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and EF1a

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCKC776

    Plasmid

    #226627

    Purpose

    piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, mCherry
    

    Use

    Synthetic Biology

    Tags

    oxygen dependent degron (ODD)

    Expression

    Mammalian

    Mutation

    Promoter

    4xHRE_YB TATA and EF1a

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV_TRE3G_hRTN3E-Halo

    Plasmid

    #216252

    Purpose

    Tet-inducible hRTN3E (long form) expression vector, expresses hRTN3E-TEV-Halo, Lentiviral vector

    Depositor

    Edward Avezov

    Article

    Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

    Insert

    reticulon-3 isoform e (RTN3 Human)
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mutation

    Promoter

    TRE3G

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. sgRNA1_Cas9-hGem_Smc4

    Plasmid

    #217667

    Purpose

    expresses Cas9-hGem and guideRNA for tagging of SMC4

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    sgRNA1 targeting SMC4 C-terminal (SMC4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHDR_NPM2-T2A-mGreenLantern-PuroTK

    Plasmid

    #222910

    Purpose

    Homology directed repair template for knocking in mGreenLantern reporter to NPM2.

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    mGreenLantern
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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