We narrowed to 34,404 results for: CaS;

1. SpCas9-NRRH

   Plasmid

   #198540

   Purpose

   Expresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRRH
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpCas9-NRCH

   Plasmid

   #198542

   Purpose

   Expresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas3-Apr

   Plasmid

   #208000

   Purpose

   Expresses all necessary components of Type I-C CRISPR-Cas system

   Depositor

   Pablo Ivan Nikel

   Article

   Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

   Inserts

   RhaR (rhaR )
   RhaS (rhaS )
   pRhaBAD
   I-C crRNA
   Cas3
   Cas5
   Cas8
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. DG SaCas9 GFP V3

   Plasmid

   #226961

   Purpose

   CBh-SaCas9-2A-GFP, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SaCas9 GFP V3

   Plasmid

   #226962

   Purpose

   CBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. DG SaCas9 puro V3

   Plasmid

   #226960

   Purpose

   CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. dCas9-FCPF

   Plasmid

   #220131

   Purpose

   Expresses dCas9-FCPF in mammalian cells

   Depositor

   Xinlai Cheng

   Article

   Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

   Insert

   dCas9-FCPF (cas9 S. pyogenes, Synthetic)
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRS-dCas9

   Plasmid

   #220193

   Purpose

   mini-Tn7 dCas9 expression vector

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Pseudomonas aeruginosa codon optimized

   Promoter

   promoterless

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. sgRNA1_Cas9-hGem_Smc4

   Plasmid

   #217667

   Purpose

   expresses Cas9-hGem and guideRNA for tagging of SMC4

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   sgRNA1 targeting SMC4 C-terminal (SMC4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

    Plasmid

    #222987

    Purpose

    Bacterial expression of iGeoCas9(C1) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    CL7_2xNLS_iGeoCas9(C1)_2xNLS_His
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR-CASK

    Plasmid

    #221427

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    CASK (CASK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cas9-FCPF

    Plasmid

    #220141

    Purpose

    Expresses Cas9-FCPF in mammalian cells

    Depositor

    Xinlai Cheng

    Article

    Gama-Brambila et al JACS Au. 2021 May 12;1(6):777-785. doi:

    Insert

    Cas9-FCPF (cas9 S. Pyogenes, Synthetic)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSAVED-CHAT_PCaspase

    Plasmid

    #214043

    Purpose

    Bacterial expression plasmid for SAVED-CHAT and PCaspase N-terminal fragment (aa 1-153) from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lacUV5 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSAVED-CHAT_PCaspase_PCi

    Plasmid

    #214049

    Purpose

    Bacterial expression plasmid for SAVED-CHAT, PCaspase, and PCi from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-Pcaspase-PCi
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lacUV5 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ-Un1Cas12f1

    Plasmid

    #205143

    Purpose

    Un1Cas12f1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Plant Genome. 2024 May 28:e20465. doi: 1

    Insert

    Un1Cas12f1
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTniQ-Cascade

    Plasmid

    #199659

    Purpose

    type I-F Cascade over-expression

    Depositor

    IIya Finkelstein

    Insert

    TniQ cas8 cas7 cas6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCLV3Cas-LT2

    Plasmid

    #210550

    Purpose

    Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT2::mCherrySSM
    

    Use

    Tags

    Expression

    Plant

    Mutation

    1bp insertion after AAVS1 target sequence that di…

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. ipYUC2Cas-LT1

    Plasmid

    #210559

    Purpose

    Binary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by M. polymorpha YUC2 promoter.

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pMpEF1::XVE::UBQ10t::LexA::SpCas9::PEA3AT::pU6::AAVS1gRNA::pMpEF1::LT1::Hygromycin
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF1Cas-LT1

    Plasmid

    #210556

    Purpose

    Binary vector expressing the Lineage Tracing system. Cas9 expression driven by M. polymorpha EF1 promoter. LT1 reporter under control of MpEF1p

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pMpEF1::Cas9::PEA3AT::U6p::AAVS1gRNA::EF1p::LT1::Hygromycin
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGGC-LexACasPea

    Plasmid

    #210539

    Purpose

    Cloning estradiol-inducible expression cassette containi LexA XVE binding sequence followed by SpCas9 CDS and PEA3AT as GreenGate module C

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    LexA::SpCas9::PEA3AT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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