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We narrowed to 2,481 results for: D10

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Showing: 1301 - 1320 of 2481 results

  4. pCMV-ABEMAX-NLS-P2A_BLAST

    Plasmid
    #135355
    Purpose
    Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance
    Depositor
    Alex Hewitt
    Publication
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABEMax-2XNLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    WT
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135355-plasmid-map-sequence-id-267704

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  5. pBJG1 T7-8xHis-OGT D554N

    Plasmid
    #154279
    Purpose
    Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Publication
    Janetzko et al Nat Chem Biol. 2016 Nov;12(11):899-901.
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    Aspartate 554 changed to Asparagine- bacterial co…
    Available since
    Sept. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    154279-plasmid-map-sequence-id-304834

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  7. pBiT3.1-N_ATG16L1:1-607

    Plasmid
    #211234
    Purpose
    Mammalian expression of full-length human ATG16L1. N-terminal HiBiT tag.
    Depositor
    Cheryl Arrowsmith
    Publication
    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATG16L1:M1-Y607 (ATG16L1 Human)
    Tags
    MVSGWRLFKKISGSSGGSSG
    Expression
    Mammalian
    Mutation
    T300A natural variant (VAR_021834)
    Promoter
    CMV
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211234-plasmid-map-sequence-id-425928

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  16. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61364-plasmid-map-sequence-id-99800

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  17. peSCBE3-NG-Hypa

    Plasmid
    #218165
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces
    Depositor
    Yuhui Sun
    Publication
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218165-plasmid-map-sequence-id-433201

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  18. pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

    Plasmid
    #132560
    Purpose
    Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.
    Depositor
    Baisong Lu
    Publication
    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ABEmax
    sgRNA, with Tetraloop replaced by com aptamer
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    132560-plasmid-map-sequence-id-259542

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  19. BE3-hA3A-R128A

    Plasmid
    #132944
    Purpose
    Gene editing. See Gene/Insert section of the plasmid page for targeting sequence cloned into this plasmid.
    Depositor
    Hui Yang
    Publication
    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE3-hA3A(R128A)
    Use
    CRISPR; Base editor
    Expression
    Mammalian
    Mutation
    BE3-hA3A(R128A)
    Available since
    Nov. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    132944-plasmid-map-sequence-id-314824

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Showing: 1301 - 1320 of 2481 results