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We narrowed to 2,015 results for: D10

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Showing: 1301 - 1320 of 2015 results

  3. pFB1.HMBP-Prs.AEBP2iso2.mt2K

    Plasmid
    #247010
    Purpose
    Expresses human AEBP2iso2.mt2K (with twelve asp/glu-to-lys mutations) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
    Depositor
    Chen Davidovich
    Article
    Mucha et al EMBO J. 2025 Dec;44(23):6979-7020. doi:
    Insert
    AEBP2iso2.mt2K (AEBP2 Human)
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    E95K, D96K, E97K, D98K, E99K, E100K, E101K, D102K…
    Promoter
    Polyhedrin
    Available Since
    Nov. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    247010-plasmid-map-sequence-id-493366

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  4. pFB1.HMBP-Prs.AEBP2iso2.mt2A

    Plasmid
    #247009
    Purpose
    Expresses human AEBP2iso2.mt2A (with fifteen asp/glu-to-ala mutations) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
    Depositor
    Chen Davidovich
    Article
    Mucha et al EMBO J. 2025 Dec;44(23):6979-7020. doi:
    Insert
    AEBP2iso2.mt2A (AEBP2 Human)
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    E95A, D96A, E97A, D98A, E99A, E100A, E101A, D102A…
    Promoter
    Polyhedrin
    Available Since
    Nov. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    247009-plasmid-map-sequence-id-493364

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  5. pKb-POC1-CBE

    Plasmid
    #255131
    Purpose
    Plant expression of A3A(Y130F)-OpenCRISPR1 D10A-UGI for cytosine base editing.
    Depositor
    Kutubuddin Molla
    Article
    Das et al New Phytol. 2026 May 21. doi: 10.1111/np
    Insert
    A3A(Y130F) fused at 5' of the Rice codon optimized pOC1 D10A and UGI at the 3' end
    Tags
    NA
    Expression
    Plant
    Promoter
    Maize ubiquitin promoter for A3A(Y130F)-OpenCRISP…
    Available Since
    July 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    255131-plasmid-map-sequence-id-516879

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  6. N-term AAV-ABE8e-SpCas9(S55R) (CA21)

    Plasmid
    #242659
    Purpose
    CBh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-SpCas9(S55R) - to be used with eVRQR constructs
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCBh-BPNLS(SV40)-TadA8e-gs-SpCas9(D10A;S55R)-[N-term]-NpuN-BPNLS(SV40)-WPRE-bGH_PA
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Mutation
    SpCas9(S55R)
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242659-plasmid-map-sequence-id-489035

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  7. pGBW-m4095421

    Plasmid
    #149524
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 nsp3
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    d1-745 d1063-1945
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149524-plasmid-map-sequence-id-307625

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  8. pcDNA3.1(+) Laccase2 MCS-mFndc3b Mutant miRNAs

    Plasmid
    #126444
    Purpose
    Expresses a mutated version of mouse Fndc3b circular RNA with the miR-93-3p, miR-258-5p, and miR-412-3p target sites all mutated
    Depositor
    Jeremy Wilusz
    Article
    Garikipati et al Nat Commun. 2019 Sep 20;10(1):4317. doi:
    Insert
    Fndc3b (Fndc3b Mouse)
    Expression
    Mammalian
    Mutation
    circFndc3b exons are separated by a miniature int…
    Promoter
    CMV
    Available Since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    126444-plasmid-map-sequence-id-245088

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  9. pFB1.HMBP-Prs.AEBP2iso2.mt1K2K

    Plasmid
    #247013
    Purpose
    Expresses human AEBP2iso2.mt1K2K (with 27 asp/glu-to-lys mutations) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
    Depositor
    Chen Davidovich
    Article
    Mucha et al EMBO J. 2025 Dec;44(23):6979-7020. doi:
    Insert
    AEBP2iso2.mt1K2K (AEBP2 Human)
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    E37K, E38K, E39K, E40K, E41K, E42K, E43K, E44K, E…
    Promoter
    Polyhedrin
    Available Since
    Nov. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    247013-plasmid-map-sequence-id-493367

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  10. pFB1.HMBP-Prs.AEBP2iso2.mt1A2A

    Plasmid
    #247012
    Purpose
    Expresses human AEBP2iso2.mt1A2A (with 27 asp/glu-to-ala mutations) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
    Depositor
    Chen Davidovich
    Article
    Mucha et al EMBO J. 2025 Dec;44(23):6979-7020. doi:
    Insert
    AEBP2iso2.mt1A2A (AEBP2 Human)
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    E37A, E38A, E39A, E40A, E41A, E42A, E43A, E44A, E…
    Promoter
    Polyhedrin
    Available Since
    Nov. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    247012-plasmid-map-sequence-id-493365

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  11. pCMV-T7-ABE8e-SpCas9(LWRYEK)-P2A-EGFP (BKS1028)

    Plasmid
    #223114
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA8e-SpCas9(LWRYEK)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/LWRYEK)=D1135L, S1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223114-plasmid-map-sequence-id-447638

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  12. peSCBE3-NG-HF1-Hypa

    Plasmid
    #218166
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-HF1-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218166-plasmid-map-sequence-id-433270

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  14. pCMV-T7-TadCBEd-SpCas9(LWKYQS)-P2A-EGFP (BKS1014)

    Plasmid
    #223127
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(LWKYQS)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/LWKYQS)=D1135L, …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223127-plasmid-map-sequence-id-447695

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  15. pSLPB2B-ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187950
    Purpose
    ecDHFR degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187950-plasmid-map-sequence-id-373501

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  16. GCN5 core-dCas9-CBP core

    Plasmid
    #179555
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179555-plasmid-map-sequence-id-354784

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  17. GCN5 core-dCas9-p300 core

    Plasmid
    #179553
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179553-plasmid-map-sequence-id-354783

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  18. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid
    #171089
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Mutation
    D10A, H840A
    Promoter
    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171089-plasmid-map-sequence-id-339962

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  19. pSLPB3B-AID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

    Plasmid
    #187960
    Purpose
    AID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187960-plasmid-map-sequence-id-373524

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  20. FRIG-myc-Sema4D-CD4

    Plasmid
    #51606
    Purpose
    expresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backbone
    Depositor
    Suzanne Paradis
    Article
    Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do
    Insert
    Sema4D/CD4-FLAG (Sema4d Mouse, Human)
    Use
    Lentiviral
    Tags
    FLAG and myc
    Expression
    Mammalian
    Mutation
    Sema4D amino acids 660-861 were replaced with CD4…
    Promoter
    RSV
    Available Since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 1301 - 1320 of 2015 results