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Plasmid#102466PurposeExpresses sigma 54 protein in E. coli. The expression is controlled by a OR2-OR1-Pr promoter.DepositorArticleInsertsigma 54 (rpoN )
UseTagsExpressionBacterialMutationPromoterOR2-OR1-PrAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJPC12-ΔM13-mCherry-PR/PRM-GFP
Plasmid#80859PurposeReporter plasmid (mCherry and GFP)DepositorInsertsmCherry
GFP
UseTagsExpressionBacterialMutationPromoterLambda Pr and Lambda PrmAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pR I-Ab beta Derp1-117
Plasmid#113639PurposeInsect cell expression vector for I-Ab beta chain fused to Der p 1 117-127 antigenic peptideDepositorInsertI-Ab beta Derp1-117 (H2-Ab1 Mouse)
UseTags6x His tag, fused to Der p 1 (117-127) peptide vi…ExpressionInsectMutationtransmembrane and cytoplasmic domains truncatedPromoterMetallothioneinAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)
Plasmid#70191PurposeDerived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.DepositorInsertClpX (clpX )
UseTagsExpressionMutationPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-OR2-OR1-Pr-UTR1-deGFP-T500
Plasmid#40019DepositorInsertdeGFP
UseTagsExpressionBacterialMutationPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available sinceOct. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP PR-D25A
Plasmid#101334PurposepBS NL4-3 envFS eGFP with mutation that disrupts Protease catalytic activityDepositorInsertNL4-3 envFS
UseLentiviralTagsExpressionMutationProtease D25APromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9
Plasmid#186699PurposePlasmid encoding PRDM9dC-Cas9 fusion under CMV promoterDepositorInsertPRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9 (PRDM9 Human)
UseCRISPRTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)
Plasmid#99229PurposeMammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.DepositorInsertpr-mEos2
UseTagsLifeactExpressionMammalianMutationA69T for primed conversion applicationsPromoterCMVAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-OR2-OR1-Pr-UTR1-araC-T500
Plasmid#45786DepositorInsertaraC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJRL2 PHO5(UASm1)pr VYFP (EB#1549)
Plasmid#14841DepositorInsertPHO5 promoter UASm1 (PHO5 Budding Yeast)
UseTagsVYFPExpressionYeastMutationUASm1 (AAGCTT was substituted for CACGTT). Unindu…PromoterAvailable sinceMay 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9
Plasmid#184464PurposePlasmid encoding PRDM9-Cas9 fusion under CMV promoterDepositorInsertPRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9 (PRDM9 Human)
UseCRISPRTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15a-OR2-OR1-Pr-UTR1-sigma28-T500
Plasmid#45779DepositorInsertsigma factor 28
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJRL2 PHO5(TATA-A1T6)pr ECFP (EB#1560)
Plasmid#14846DepositorInsertPHO5 promoter TATA-A1T6 (PHO5 Budding Yeast)
UseTagsECFPExpressionYeastMutationTATA-A1T6. TATA box mutated from TATATAAG to AATA…PromoterAvailable sinceMay 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-OR2-OR1-Pr-UTR1-deGFPT500 (121p)
Plasmid#70197PurposePromoter panelDepositorInsertdeGFP
UseTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJRL2 PHO5(TATA-C2)pr ECFP (EB#1562)
Plasmid#14844DepositorInsertPHO5 promoter TATA-C2 (PHO5 Budding Yeast)
UseTagsECFPExpressionYeastMutationTATA-C2. TATA box mutated from TATATAAG to TCTA…PromoterAvailable sinceMay 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJRL2 GAL1(TATA-A1T6)pr VYFP (EB#1573)
Plasmid#14849DepositorInsertGAL1 promoter TATA-A1T6 (GAL1 Budding Yeast)
UseTagsVYFPExpressionYeastMutationTATA-A1T6. TATA box mutated from TATATAAG to AATA…PromoterAvailable sinceMay 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJRL2 GAL1(TATA-A1T6)pr ECFP (EB#1574)
Plasmid#14850DepositorInsertGAL1 promoter TATA-A1T6 (GAL1 Budding Yeast)
UseTagsECFPExpressionYeastMutationTATA-A1T6. TATA box mutated from TATATAAG to AATA…PromoterAvailable sinceMay 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJRL2 GAL1(TATA-C2)pr VYFP (EB#1577)
Plasmid#14851DepositorInsertGAL1 promoter TATA-C2 (GAL1 Budding Yeast)
UseTagsVYFPExpressionYeastMutationTATA-C2. TATA box mutated from TATATAAG to TCTATA…PromoterAvailable sinceMay 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJRL2 GAL1(TATA-C2)pr ECFP (EB#1578)
Plasmid#14852DepositorInsertGAL1 promoter TATA-C2 (GAL1 Budding Yeast)
UseTagsECFPExpressionYeastMutationTATA-C2. TATA box mutated from TATATAAG to TCTATA…PromoterAvailable sinceMay 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBEST-OR2-OR1-Pr-araC, pBAD-TetR, pBAD-TetO1-deGFP-ssrA
Plasmid#45789DepositorInsertsdeGFP
Tetracycline repressor
araC
UseSynthetic BiologyTagsssrA degradation tagExpressionBacterialMutationPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only