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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 5,441 results for: SPI

Showing: 1301 - 1320 of 5441 results

  1. pTol2-Ubi-lsl-EGFP-nls-mCherry-hsp70-zCreI-mTagBFP2 (JDW 1235)

    Plasmid
    #229814
    Purpose
    A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with hsp70-TagBFP-zCre in the opposite direction.
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    loxp-EGFP-stop-lox
    Use
    Cre/Lox; Tol2 based expression vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229814-plasmid-map-sequence-id-460474

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  2. pTol2-Ubi-lsl-EGFP-nls-mCherry:fli1aep-CreI-zf1-BFP (JDW 1236)

    Plasmid
    #229816
    Purpose
    A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with fli1-TagBFP-zCre in the opposite direction.
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    loxp-EGFP-stop-lox
    Use
    Tol2 based expression vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229816-plasmid-map-sequence-id-460476

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  4. pTol2-Hsp70-H2B-mCerulean-lsl-mScarlet-fli1-zCre-I-BFP (JDW 1233)

    Plasmid
    #229841
    Purpose
    A Tol2 based expression vector with the Hsp70 promoter driving a cre dependent switch reporter. Contains an endothelial driven cre
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    loxp-H2B-mCerulean-2xStop-loxP
    Use
    Cre/Lox; Tol2 based expression vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229841-plasmid-map-sequence-id-461093

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  5. AP2u2-CMV-S_protein

    Plasmid
    #226456
    Purpose
    Mammalian expression of S protein of SARS-CoV2 under CMV promoter, tagged with 6His and released extracellular environment after expression for protein purification
    Depositor
    Urartu Seker
    Article
    Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(
    Insert
    Spike Protein
    Use
    Tags
    6xHis Tag, Foldon Domain, and Thrombin Cleavage S…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    226456-plasmid-map-sequence-id-458388

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  6. pME-Actin-Vhh-sfGFP-P2A-1xHA-iRFP-caax (JDW 1216)

    Plasmid
    #224496
    Purpose
    Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and iRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    Actin-Vhh-sfGFP-P2A-HA-iRFP-caax
    Use
    Gateway cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    224496-plasmid-map-sequence-id-447065

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  7. pORANGE_Tubb3 GFP(Y39TAG) KI

    Plasmid
    #182678
    Purpose
    Expression of spCas9, gRNA targeting the end of Tubb3 gene and donor GFP(Y39TAG). Can be used for amber codon suppression and click chemistry labeling of endogenous beta-3 tubulin in mammalian cells.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Tubb3-targeting gRNA and GFP(Y39TAG) donor sequence (Tubb3, Tubb3 Rat, Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182678-plasmid-map-sequence-id-363084

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  8. pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(TAG)-3xFLAG donor

    Plasmid
    #182686
    Purpose
    Contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linkerA6TAG-3xFLAG. Can be used as a donor for the tagging of endogenous NFL via TKIT approach and click labeling of NFL.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse Nefl intron3-exon4-linker(TAG)-3xFLAG donor sequence (Nefl Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    NA
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182686-plasmid-map-sequence-id-363076

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  12. SARS-CoV2 Nucleocapsid

    Plasmid
    #153201
    Purpose
    Expresses myc-tagged nucleocapsid (N) from SARS-CoV2 in mammalian cells
    Depositor
    Peter Klein
    Article
    Liu et al Proc Natl Acad Sci U S A. 2021 Oct 19;11
    Insert
    Nucleocapsid from SARS-CoV-2 (N severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
    Use
    Tags
    Myc tag (5X)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 15, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153201-plasmid-map-sequence-id-305776

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  14. plenti-Lb-Cas12a-2xNLS

    Plasmid
    #155046
    Purpose
    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS, Myc-tag and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    155046-plasmid-map-sequence-id-308670

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  16. pMXs-IRES-blast 3xFlag-SPNS1(E164K)

    Plasmid
    #198569
    Purpose
    SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector
    Depositor
    Monther Abu-Remaileh
    Article
    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi
    Insert
    SPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
    Use
    Retroviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    changed glutamic acid E164 to lysine (E164K)
    Promoter
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    198569-plasmid-map-sequence-id-438870

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  20. pMXs-IRES-blast GFP-SPNS1(E164K)

    Plasmid
    #198572
    Purpose
    SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector
    Depositor
    Monther Abu-Remaileh
    Article
    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi
    Insert
    SPNS1(E164K) with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
    Use
    Retroviral
    Tags
    3xFlag-GFP
    Expression
    Mammalian
    Mutation
    changed glutamic acid E164 to lysine (E164K)
    Promoter
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    198572-plasmid-map-sequence-id-438878

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Showing: 1301 - 1320 of 5441 results