We narrowed to 4,161 results for: biorxiv

1. pH6HTC_CCDC59-Halo

   Plasmid

   #175327

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   CCDC59 (CCDC59 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJYDNgp

   Plasmid

   #162453

   Purpose

   Positive control plasmid for pJYDNg carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid contains a long 2G linker.

   Depositor

   Gideon Schreiber

   Article

   Zahradník et al bioRxiv Preprint 2020

   Insert

   Aga2p-2Glinker-eUnaG2 (AGA2 )
   

   Use

   Shuttle vector bacteria/yeast

   Tags

   HA nad myc and eUnaG2 fluorescent protein

   Expression

   Mutation

   Promoter

   GAL1

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL0_19 [panARS ORI]

   Plasmid

   #198932

   Purpose

   Level 0 part

   Depositor

   Charlie Gilbert, Nili Ostrov, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   panARS ORI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. hTspan12(1-252)-6xHis-3C-sGFP11_pVL1393

   Plasmid

   #216382

   Purpose

   Baculovirus transfer vector to express human Tspan12 ∆C (residues 1-252) fused to split GFP s11

   Depositor

   William Weis

   Article

   Bruguera et al bioRxiv 2024.02.03.578714

   Insert

   Tetraspanin-12 (TSPAN12 Human)
   

   Use

   Baculovirus

   Tags

   6xHis-3C-splitGFP11

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. MBP-3C-hNorrin(33-133)-1D4_pAcGP67a

   Plasmid

   #216384

   Purpose

   Baculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218)

   Depositor

   William Weis

   Article

   Bruguera et al bioRxiv 2024.02.03.578714

   Insert

   Norrin (NDP Human)
   

   Use

   Baculovirus

   Tags

   GP64 signal sequence-MBP-3C and Rho1D4

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pH6HTC_mmHUR-Halo

   Plasmid

   #175311

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   HUR (Elavl1 Mouse)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pH6HTC_PEBP1-Halo

   Plasmid

   #175333

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   PEBP1 (PEBP1 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE30-TEV-pro-conA

   Plasmid

   #159527

   Purpose

   Expresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.

   Depositor

   Joshua Mylne

   Article

   Nonis et al bioRxiv. 2020.

   Insert

   Canavalia ensiformis pro-concanavalin A
   

   Use

   Tags

   Six-Histidine tag and tobacco etch virus (TEV) pr…

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. 3xmTurquoise2_UtrCH

   Plasmid

   #112963

   Purpose

   In vivo visualization of F-actin (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv 160374

   Insert

   UtrCH
   

   Use

   Tags

   3x mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-TagBFP-P2A-Pre-SPHERE-SF-iGluSnFR

    Plasmid

    #187089

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Pre-SPHERE-SF-iGluSnFR
    

    Use

    Tags

    TagBFP-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-EgI-NeoR

    Plasmid

    #207175

    Purpose

    Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-NeoR

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-dSa-VP64

    Plasmid

    #99680

    Purpose

    Expresses dSa Cas9 fused to gold standard VP64 activator

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VP64

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Oct. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-TagBFP-P2A-Pre-SPHERE-iGABASnFR

    Plasmid

    #187091

    Purpose

    GABA sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Pre-SPHERE-iGABASnFR
    

    Use

    Tags

    TagBFP-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-iRFP-P2A-Post-SPHERE-iGABASnFR

    Plasmid

    #187092

    Purpose

    GABA sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Post-SPHERE-iGABASnFR
    

    Use

    Tags

    iRFP670-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-iRFP-P2A-Post-SPHERE-SF-iGluSnFR

    Plasmid

    #187090

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Post-SPHERE-SF-iGluSnFR
    

    Use

    Tags

    iRFP670-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJYDN3p

    Plasmid

    #162457

    Purpose

    Positive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.

    Depositor

    Gideon Schreiber

    Article

    Zahradník et al bioRxiv Preprint 2020

    Insert

    Aga2p-eUnaG2 (AGA2 )
    

    Use

    Shuttle vector bacteria/yeast

    Tags

    ALFA tag, HA and myc tags, and eUnaG2 fluorescent…

    Expression

    Mutation

    Promoter

    GAL1

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG306-pTet07-synYFP[TTG/AGA]-nLuc

    Plasmid

    #199756

    Purpose

    Elongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stalling

    Depositor

    Brian Zid

    Article

    Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

    Insert

    synYFP[TTG/AGA]-nLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TetO7

    Available since

    May 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCEP4-DAT-myc

    Plasmid

    #107457

    Purpose

    Mammalian expression plasmid for codon optimized hDAT with internal MYC tag inserted in ECL2

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Dopamine Transporter (DAT) (SLC6A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-CMV-Pink Flamindo-p2a-paGFP-bPAC-CAAX

    Plasmid

    #202718

    Purpose

    Mammalian co-expression of soluble Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imaging

    Depositor

    Adam Cohen

    Article

    Xiang et al bioRxiv 2023

    Inserts

    Pink Flamindo
    paGFP-bPAC-CAAX
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL0_13 [pIP501 ORI]

    Plasmid

    #198927

    Purpose

    Level 0 part

    Depositor

    Charlie Gilbert, Nili Ostrov, Cultivarium Tools

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pIP501 ORI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge